... based on the method byCawthon et al[33]. Briefly, commercially obtained telo-mere specific primers; CGGTTTGT TTGGGTTTGGGTTTGGGTTTGGGTTTGGGTT (forward) and GGCTTGCCTTACCCTTACCCTTACCCTTACCCTTACCCT ... 2008,129:60-66.doi:10.1186/1423-0127-18-41Cite this article as: Ngom et al.: Thymic function and T cell parameters in a natural human experimental model of seasonal infectious diseases and nutritional burden. Journal of Biomedical Science ... separate into anaqueous RNA phase, an organic protein layer and a DNA interphase.RNA was extracted by adding 0.5 ml isopropanol tothe aqueous phase and incubating at-20°C overnight,then centri...