... harbored the Sox9-binding sites of the AP-2e promoter (Sox9_1 and Sox9_2). Incubation of in vitro-synthesized Sox9 with the labeled oligonucleo-tides containing the Sox9-binding sites resulted in ... factor-binding sites, we screened approximately1 kb of DNA of the upstream regulatory region, using the matinspector (Genomatix Software GmbH, Munich,Germany). We determined the start site of transcriptionby ... isexpressed in limb buds during early embryogenesis, in the growth plate, and in chondrocytes of the joints[36]. The AP-2a knockout mouse died at birth, withsevere malformations of the craniofacial...