... only the novel data: protonation constantsfor DAHKam and VIHN, and stability constants (log a values) of Cu(II)-VIHN, Cu-DAHKam and Ni-DAHKamsystems. The parameters of CD and EPR spectra of allmajor ... assessed by HPLC analysis of thefinal m aterials. I dentity and purity of peptide was confirmed by mass spectrometry, utilizing a Finnigan MAT TSQ 700(Finnigan MAT, San Jose, CA, USA) mass spectrometerequipped ... Poland;2Institute of Biochemistry and Biophysics, Polish Academy of Sciences,Warsaw, Poland A comparative study of thermodynamic and kinetic aspects of Cu(II) and Ni(II) binding at the N-terminal b inding...