báo cáo khoa học: "Chromosomal analysis of selected lines of Drosophila melanogaster with a new level of bristle canalization Eva" doc

... Chromosomal analysis of selected lines of Drosophila melanogaster with a new level of bristle canalization Eva GARC A- VÁZQUEZJ. RUBIODepartamento de Biologia Funcional (Area de ... widelystudied as a model of canalized characters. Practically all the individuals of naturalpopulations have the normal phenotype (fig. 1), although some are not canalized andhave ... ADC-7, BAB has somepositional decanalization, and ABB is clearly positionally and numerically decanalized.These observations are statistically proved by positional standard...

Báo cáo khoa học: "Whole shoot hydraulic resistance in Quercus species measured with a new high-pressure flowmeter" pps

... (Tyree et al, 1993) and for Acersaccharum and Populus deltoides (Tyreeand Alexander, unpublished data). Theleaf-blade resistance includes vascularand nonvascular pathways from ... ifmost water evaporation occurs near thestomata in accordance with the evidencein support of peristomatal evaporation insubstomatal cavities (Tyree and Yianoulis,1980; Yianoulis ... system made fromthick-walled glass capillary tubes of 1.5 mm in-ternal diameter. The water level in the right arm of the manometer (MR) was always at the same level as the...

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... 5-GACGAGATTATCAGATTTTACGC-3 and 5-AACTGGTGAAGCGGAAGAGAC-3, PTI1-4 (At2g47060): 5-CCCCAAAGAAAATGAGTTGCT-3 and 5-GCATCATTTCCTGGAGGAAAG-3.AcknowledgementThis project was supported by grants from the Aus-trian Science ... ACTIN2gene as an internal standard. PCRs were performed usingthe following primers: ACT2 (At3g18780): 5-ACATTGTGCTCAGTGGTGGA-3 and 5-CTGAGGGAAGCAAGAATGGA-3, OXI1 (At3g25250): 5-GACGAGATTATCAGATTTTACGC-3 ... 565–577.26 Takahashi A, Agrawal GK, Yamazaki M, Onosato K,Miyao A, Kawasaki T, Shimamoto K & Hirochika H(2007) Rice Pti 1a negatively regulates RAR1-dependentdefense responses. Plant Cell...

Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt

... were of analytical grade from Nacalai Tesque (Kyoto, Japan) andWako Pure Chemical Industries (Osaka, Japan).Culture and screening of bacteriaBacterial strains were cultivated for 15–20 h at ... a- oxobutyrate, ﬂuoropyruvate, a- oxovalerate, a- oxoisocaproate, a- oxo-octanoate, and hydroxypyru-vate. However, branched-chain a- oxo acids, such as a- oxoisovalerate and a- oxo-b-methylvalerate, wereinert. ... myokinase, enolase, lactate dehydrogenase, andglutamate dehydrogenase from Oriental Yeast, Osaka,Japan.Other analytical methodsThe N-terminal amino-acid sequence of the enzyme wasdetermined with...

Báo cáo khoa học: Post-ischemic brain damage: NF-jB dimer heterogeneity as a molecular determinant of neuron vulnerability pdf

... Kitagawa-Sakakida S, Nishimura M,Morishita R, Kaneda Y, Kohmura E, Yoshimine T &Matsuda H (2001) Nuclear factor-kappa B decoy atten-uates neuronal damage after global brain ischemia: a future ... A requirement of nuclear factor-kappaB activation in fear-potentiated startle. J Biol Chem 277, 46720–46729.39 Yeh SH, Lin CH & Gean PW (2004) Acetylation of nuclear factor-kappaB in rat amygdala improves ... signal transducer and activator of tran-scription 3 and nuclear factor-kappaB (NF-j B) [1].NF-jB is a key regulator of both inﬂammation andcell death and has been proposed as suitable target...

Báo cáo khoa học: MicroRNA-23b mediates urokinase and c-met downmodulation and a decreased migration of human hepatocellular carcinoma cells doc

... degradation, a semiquantitativeRT-PCR evaluation was carried out on RNA isolatedfrom control and transfected cells. The data showedcomparable steady-state mRNA levels of uPAand c-met (Fig. 4A, B), ... trypsinized, andcounted using a Burker chamber.Statistical analysis Each experiment was carried out at least twice. Histogramsrepresent the mean values, and bars indicate standarderrors of the mean. ... control.RT-PCR analysis (Agilent) and real-time PCRTotal RNA of transfected and nontransfected cells wasextracted with Trizol reagent according to the manufacturer’smiR-23b downmodulates uPA and c-met A. ...

Báo cáo khoa học: "Lymphatic mapping and sentinel node biopsy in gynecological cancers: a critical review of the literature" pot

... pre-sacral andthen para-aortic nodes. Although majority of the nodesare located in internal iliac and external iliac areas, nodeshave been found in also presacral, parametrial and parar-ectal ... patients with early stage cervical cancer. Gynecol Oncol 2003, 90:290-296.50. Hubalewska A, Sowa-Staszczak A, Huszno B, Markocka A, Pityñski K,Basta A, Opławski M, Basta P: Use of Tc-99m-nanocolloid ... isosulfan blue, have causeddisappointment at first [1]. DeCesare et al., demonstratedthe applicability of intra-operative gamma ray use, and a year later, Hullu et al., demonstrated the applicability...

Tài liệu Báo cáo khoa học: Puriﬁed RPE65 shows isomerohydrolase activity after reassociation with a phospholipid membrane pdf

... increasingconcentrations of Chaps, higher concentrations of Chaps also abolished the enzymatic activity of RPE65.By careful titration, we found that Chaps at a concen-tration of 0.1% was optimal ... 5414–5418.36 Bok D, Ruiz A, Yaron O, Jahng WJ, Ray A, Xue L &Rando RR (2003) Purification and characterization of a transmembrane domain-deleted form of lecithin retinolacyltransferase. Biochemistry ... lipid amount in each flotation fractionwas quantified by scintillation counting of [14C]PC and expressed as a percentage of the total amount of [14C]PC in the gradient(means ± standard deviation,...

Báo cáo khoa học: Enhancing the protein production levels in Escherichia coli with a strong promoter potx

... ACACCC-ATGGAGCTTTCCTGTGTGAAATTGT, lacUV5 wasamplified. TEHA3: ACACAGATCTCTGCAGTGAAATG-AGCTGTTGACAATTA and TEHA4: ACACCCATGGT-CTGTTTCCTGTG were used for trc amplification. Theexact nucleotide sequence of each promoter region ... was amplified from thevector pAff8eGFP using TEHA7: ACACCTGCAGCGAT-CCCGCGAAATTAATAC and TEHA8: ACACCCATGG-TATATCTCCTTCT, introducing restriction sites for PstIupstream and NcoI downstream of ... promoters lacUV5 and trcwere amplified by PCR from vectors including the relevantgenes. By using primers TEHA1: ACACAGATCTCTGCA-GGGCACCCCAGGCTTTACA and TEHA2: ACACCC-ATGGAGCTTTCCTGTGTGAAATTGT, lacUV5...

Báo cáo khoa học: The highly conserved extracellular peptide, DSYG(893–896), is a critical structure for sodium pump function docx

... type5¢-GTGGAGGACAGCTATGGGCAGCAG-3¢Asp893fiArg5¢-GTGGAGCGCAGCTATGGGCAGCAG-3¢Asp893fiGlu5¢-GTGGAGGAGAGCTATGGGCAGCAG-3¢Asp893fiAla5¢-GTGGAGGCCAGCTATGGGCAGCAG-3¢Ser894fiAsp5¢-GTGGAGGACGACTATGGGCAGCAG-3¢Ser894fiIle5¢-GTGGAGGACATCTATGGGCAGCAG-3Gly896fiArg ... type5¢-GTGGAGGACAGCTATGGGCAGCAG-3¢Asp893fiArg5¢-GTGGAGCGCAGCTATGGGCAGCAG-3¢Asp893fiGlu5¢-GTGGAGGAGAGCTATGGGCAGCAG-3¢Asp893fiAla5¢-GTGGAGGCCAGCTATGGGCAGCAG-3¢Ser894fiAsp5¢-GTGGAGGACGACTATGGGCAGCAG-3¢Ser894fiIle5¢-GTGGAGGACATCTATGGGCAGCAG-3Gly896fiArg 5¢-GTGGAGGACAGCTATAGGCAGCAG-3¢Gly896fiIle5¢-GTGGAGGACAGCTATATCCAGCAG-3¢Second primer for all of the above5¢-GTCATTAATCCAACGGTCATCCCA-3¢ a Primers used for mutations ... primer5¢-CCACTGCTGCCCACAGCTGTCCTCCAC-3¢Tyr895fiPhe5¢-CGATGTGGAGGACAGCTTTGGCCAGCAGTGGACCTATG-3¢Second primer5¢-CATAGGTCCACTGCTGGCCAAAGCTGTCCTCCACATCG-3¢bTyr895fiTrp 5¢-CGATGTGGAGGACAGCTGGGGGCAGCAGTGGACC-3¢Second primer5¢-GGTCCACTGCTGCCCCCAGCTGTCCTCCACATCG-3¢ a Silent...

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