Báo cáo y học: "Two different genomes that produce the same result" docx

... say it was the very ﬁrst, but nearly 45 years ago, George Streisinger and his colleagues mutated the lysozyme gene of phage T4 with acridine, which they knew caused frameshifts, and then they ... formation of the stalk and the synthesis of a purple dye in the spore mass. Both species aggregate by chemotaxis toward sources of cAMP. The high synchrony of development makes these experiments ... acids encoded by the DNA between the two mutations should, in theory, have been changed - and they were. Knowing the changed amino acids and the genetic code, the group determined the actual DNA...

Báo cáo Y học: Two different E2F6 proteins generated by alternative splicing and internal translation initiation ppt

... compared itsactivity to the activity of Exon2-luc in transient transfectionassays. The mutation led to complete loss of activity in theseassays, indicating that the integrity of the AUG is crucial ... oligo(dT) primer was used in the previous study. We also show that the ratio between the twoE2F6 mRNAs does not change signiﬁcantly during the cellcycle. The E2F6b protein lacks the amino-terminal 36 ... 3B,C).Because of the lower afﬁnity of the N14 antiserum, wecannot completely exclude the possibility that the band in the C10 immunoprecipitation corresponding in size toE2F6b is a proteolytic breakdown...

Báo cáo y học: "Do methadone and buprenorphine have the same impact on psychopathological symptoms of heroin addicts" pdf

... in the psycho-pathology of addiction, but also taking into accountsymptoms and syndromes that are under the thresholdfor the definition of an additional mental disorder,although they may have ... specific therapeutic actiondisplayed by opioid agents on psychopathological symptoms. In the present report we compared the effects ofbuprenorphine and methadone on the psychopathological symptoms ... pathophysiology of psy-chotic disorders [43]. The antipsychotic effectiveness ofopiate agonists [44] is supported by the fact that metha-done maintenance i s responsible for the prevention ofpsychotic...

Báo cáo y học: "Two-year Outcome of Turkish Patients Treated with Zotarolimus Versus Paclitaxel Eluting Stents in an Unselected Population with Coronary Artery Disease in the Real World: A Prospective Non-randomized Registry in Southern Turk

... antiplatelet therapy and this likely played a role in the observed MACE events. Also noteworthy was the observation that, the lesion and the stent lengths recorded in our study were significantly longer ... methods. The operators were free to use the stent ap-proach and either the ZES or PES stent that they con-sidered to be best. Study End Points and Definitions The primary clinical efficacy end ... Furthermore, with the advent of new DES systems, it is important to elucidate any differences in efficacy and safety when utilizing the currently availab l e DESs. Therefore, we report the two-year outcomes...

Báo cáo y học: " Two-stage procedure in the treatment of late chronic hip infections spacer"

... organism but conventionally have to fulfil the criteria estab-lished by Murray [24] including: antibiotic safety, thermostability, hypoallergenicity, water solubility, adequate bactericidal ... arthro-plasty for periprosthetic infection recently and con-cluded that incorporation of liquid gentamicin in bone cement spacers led to effective drug delivery with systemic safety. The most commonly ... mind that if ALC had been used for the primary procedure, bacteria causing the infection may have already survived high con-centrations of that antibiotic and will likely be resis-tant if the...

Báo cáo Y học: Two GPX-like proteins from Lycopersicon esculentum and Helianthus annuus are antioxidant enzymes with phospholipid hydroperoxide glutathione peroxidase and thioredoxin peroxidase activities pptx

... replacement of selenocysteine bycysteine greatly reduces the activity of the enzyme [12].According to Eshdat et al. [13], this would result in a plantactivity lower by three orders of magnitude ... [25]. The small size and hydrophobic surface of these proteinscan explain that PHGPXs (GPX4) are unique in theiractivity towards hydroperoxides integrated in membranes[29], suggesting that they ... phospholipidhydroperoxides and a weaker afﬁnity towards t-butylhydroperoxide, as indicated by their respective apparentKmvalues. There was no detectable activity with hydrogenperoxide.Considering the...

Báo cáo Y học: Two independent, light-sensing two-component systems in a ﬁlamentous cyanobacterium pot

... aÔnormalÕ cyanobacterial phytochrome. The loss of the phytochrome-like absorbance during afﬁnity chromatogra-phy of the CphB/PCB complex, taken together with the absolute stability of the point-mutated ... incyanobacteria and other, nonphotosynthetic prokaryotes. The discovery of proteins with sequence similarity and the capability to bind tetrapyrrole chromophores in a mannerreminiscent of the ... proteins in the cyanobacterial cell contain anychromophore at all or, if not, whether they would becapable of covalently binding a chromophore (of the lineartetrapyrrole type or of any other structure)...

Báo cáo Y học: Two 1 : 1 binding modes for distamycin in the minor groove of d(GGCCAATTGG) docx

... These confirmed that in the refined crystal geometry the drug is rather too tightlypacked against the G19 base. The interaction can beimproved by locally relaxing the drug geometry, but nohydrogen ... twoorientations fitting the electron density equally well, one canconclude that both orientations in the minor groove areenergetically very similar, or that the resolution of the crystal structure ... rings form bifurcated hydrogen bonds with the thymine or adenine bases. The central N5(D) atom of the drug is hydrogen-bonded to the two O2 atoms of the thymine bases of the central AT steps....

Báo cáo khoa học: Two different types of hepcidins from the Japanese ﬂounder Paralichthys olivaceus ppt

... prepared and plated on the appropriate agar platesand incubated for 24 h.Hemolytic activity of synthesized Japanese floun-der hepcidins The hemolytic activity of the synthetic Japanese flounderhepcidins ... indicated that both hepci-din genes were present as single copies in the genomebecause the hybridized DNA bands were the same sizein both the genomic DNA and the BAC clones(Fig. 2A). The hybridized ... have eight cysteine residues, while type II hassix [11]. In the latter type, the first cysteine residue isdeleted and the third cysteine residue is replaced with aglycine residue. The number...

Báo cáo khoa học: "Two different types of malignant fibrous histiocytomas from pet dogs" docx

... Two different types of malignant fibrous histiocytomas 171histiocytoid cells. Inflammatory-type tumors showed bizzare histiocytoid cells concealed by inflammatory cells, including lymphocytes, ... supported the hypothesis that these tumor cells are derived from true histiocytes [8,9]. Several other studies have suggested that the immunohistologic heterogenity of MFH tumor cells indicate that ... in the central region of the lesion. The current report may be important in identifying MFHs based on histologic and immunohistologic findings and classifying the subtypes of these tumors that...

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