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Báo cáo y học: "Analog peptides of type II collagen can suppress arthritis in HLA-DR4" docx

Báo cáo y học:

Báo cáo y học: "Analog peptides of type II collagen can suppress arthritis in HLA-DR4" docx

... response to type II collagen. A panel of analog peptides containing amino acid sub-stitutions was tested for the ability to stimulate the proliferation of type II collagen (CII)-primed T cells. ... con-taining substitutions within the immunodominant determinant of type II collagen that effectively suppress autoimmune arthri-tis in DR4 transgenic mice.Both of these analog peptides contain ... witha decrease in the T helper type 1 cytokine IFN-γ. These exper-iments represent the first description of analog peptides of type II collagen recognized by T cells in the context of thehuman...
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Báo cáo Y học: The refolding of type II shikimate kinase from Erwinia chrysanthemi after denaturation in urea pot

Báo cáo Y học: The refolding of type II shikimate kinase from Erwinia chrysanthemi after denaturation in urea pot

... means of investigating the integrity of this region of the protein. In the present paper, we have undertaken a study of theunfolding and refolding of the type II SK from E. chry-santhemi, using ... of significant contaminant by nucleotide.Assay of enzyme activityThe activity of the shikimate kinase was determined by adouble coupled assay involving pyruvate kinase (PK) andlactate dehydrogenase ... [27].Unfolding and refolding of SK was performed essentiallyas described in our previous studies on type II dehydroqu-inase [28]. To study the extent of unfolding of SK, theenzyme was routinely incubated...
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Báo cáo y học: "Delayed resolution of acute inflammation during zymosan-induced arthritis in leptin-deficient mice" pptx

... we investigated the effects of leptindeficiency on zymosan-induced arthritis (ZIA), which isindependent of adaptive immunity. Arthritis was induced byinjection of zymosan into the knee joint. ... joint swelling in zymosan-induced arthritis Arthritis was induced in ob/ob and control +/? C57BL/6mice by injecting zymosan A into the right knee joint. Toassess whether leptin deficiency ... Committee of the Geneva University School of Medicine and of the Geneva Veterinarian Office.Induction of arthritis Arthritis was induced by injection of 180 µg zymosan A viaa small skin cut along...
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Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"

... immunoglobulin chains were also found to be significantly increased in aged thymocytes. Understanding the changes in gene expression in thymocytes with age may hold the key in determining thymocyte ... thymus, involution, aging, microarray, AGEMAP, thymocytes, caloric restriction Introduction The aging immune system is often characterized by a general decline in the ability to resist infection ... delay age-associated thymic involution through directly affecting the thymocytes. Endocrinology 2004;145: 2392-2401 22. Dominguez-Gerpe L and Rey-Mendez M. Evolution of the thymus size in...
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Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot

Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot

... recombinant proteincontaining the C-myc epitope followed by six histidines atthe C-terminus. A plasmid containing the wild type version of CCR5 was constructed by introducing the codingsequence ... theC-terminus of CCR5 would interfere with the protein’sability to function as a chemokine receptor or HIVcoreceptor, we first determined the binding of MIP-1a tothe CCR5-myc-his expressing cells. ... glycosaminoglycans [37].As the myc-his tag of the protein was present at theC-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction bythe...
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Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot

Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot

... access [14]. In contrast to allFe-hydrogenases including HydA of S. obliquus,theenzyme of C. reinhardtii has an interesting additionalprotein domain. A small insertion of 45 amino acidsbetween ... synthesis of s omeenzymes involved in glycolysis or sugar-phosphatemetabolism [54].HydA belongs to a new class of Fe-hydrogenasesHydA of C. reinhardtii, the first isolated gene encodinga hydrogenase ... Johanningmeier, U. & Howell, S.H. (1984) Regulation of light-harvesting chlorophyll-bi nding protein mRNA accumulation in Chlamydomonas reinhardtii. Possible in volvement of chlorophyllsynthesis...
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Báo cáo Y học: Functional integration of mitochondrial and hydrogenosomal ADP/ATP carriers in the Escherichia coli membrane reveals different biochemical characteristics for plants, mammals and anaerobic chytrids pdf

Báo cáo Y học: Functional integration of mitochondrial and hydrogenosomal ADP/ATP carriers in the Escherichia coli membrane reveals different biochemical characteristics for plants, mammals and anaerobic chytrids pdf

... expression of a variety of singlemitochondrial -type AACs in E. coli. After IPTG-inductionwe were able to obtain functional integration of the variousmitochondrial -type AACs into the cytoplasmic ... trans-ferredtonewreactionvesselsandheatedto95°Cfor10 min to prevent any further reaction. An aliquot of theexported radioactive solution was used for separation bythin-layer chromatography [23].Thin-layer chromatography of radioactively labeledadenine ... labeledadenine nucleotidesTo identify the type of adenine nucleotide exported from[a-32P]ADP or [a-32P]ATP preloaded E. coli cells, weemployed a thin-layer chromatography system accordingto...
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Báo cáo Y học: Supermolecular organization of photosystem II and its associated light-harvesting antenna in Arabidopsis thaliana docx

Báo cáo Y học: Supermolecular organization of photosystem II and its associated light-harvesting antenna in Arabidopsis thaliana docx

... packing of the supercomplex is verysimilar to the packing in the crystals, as is shown in thefitting of crystal features into the spinach type IIImegacomplex (Fig. 4F). Finally, the CP26 area in ... additionwas not performed in the analysis of the spinach crystals,where crystallinity in one specific part of a layer did notstrongly correlate to crystallinity in the other one [17]. Aftera ... the morecommon S -type trimers. No binding of L -type trimers couldbe detected. Analysis of native membrane-bound PSIIcrystals revealed a novel type of crystal with a unit cell of 25.6 Â 21.4 nm...
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Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot

Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot

... of cofactor for TDP enzymaticactivity was examined by determining the specific activity of TDP by following the cleavage of 1 mM of substrate by0.125 lM of enzyme in reaction buffer containing ... linking the active site tyrosine residue of topoisomerase I with the 3¢ terminus of DNA in topo-isomerase I–DNA complexes which accumulate duringtreatment of cancer with camptothecin. In yeast, ... produced no increase in A415, confirming that thehydrolysis of the tyrosine-DNA phosphodiester bonddetected before was indeed produced by the activity of purified recombinant huTDPND39 enzymes.The...
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Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot

Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot

... a1Gly562.6H Tyr50 a2Gly563.5H Tyr50 a2Gly562.6H Tyr50 a Ala593.5H Tyr50 a Ala592.6H Tyr50 b Ala593.5H Tyr50 b Ala59b1Tyr50 a1Gly56b2Tyr50 a1Gly56b1Tyr50 a2Gly56b2Tyr50 ... during the early stages of HIV-1replication, binding of NCp7 to the C-terminal domain(80–96)Vpr [8] may expose the N-terminal (1–39) portion of the protein and allow its tight binding to Lys-tRNAsynthetase ... preventing protein–protein interactions.The helix-turn-helix region of Vpr could also explain thecapacity of the protein to participate in nuclear transport of the proviral DNA [10], as this type...
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