Báo cáo y học: "4th meeting of the EU research network EUROME: From the identification of genes and cellular networks in murine models of arthritis to novel therapeutic intervention strategies in rheumatoid arthritis, London, UK, 9 March 2004" pot

... YSYTATYHIYDLTNGEFIR1 098 .96 (2+) 403–421 VTQDSLFYSSNEFEGYPGR The 97 kDa band obtained following biotinylated fluorophosphonate labelling of bovine chondrocytes was trypsin digested and analysed ... protein profiling: the serine hydrolases. Proc Natl Acad Sci USA 199 9, 96 :14 694 -14 699 .14. Kidd D, Liu Y, Cravatt BF: Profiling serine hydrolase activities in complex proteomes. Biochemistry 2001, ... terminus, aregion with several potential N-glycosylation sites, a cysteinerich substrate-binding domain and a stretch of 200 aminoacids at the carboxyl terminus containing the catalytic serine,aspartate...

... protein, which bind to (1fi3)-b-D-glucans, e.g. curdlan or laminarin. Afterbinding of the carbohydrate to the receptor, the expression of the gene coding for the binding protein increases. In ... taxa to echinoderms [65] or ascidians [21] in the deuterostomian branch, and to the horseshoe crabs [20] in the protostomian line; this domain is thought to play arole in carbohydrate binding ... phos-phorylation after incubation with this glucan. To determine the specificity of this reaction, the known tyrosine kinaseinhibitor, aeroplysinin, isolated from the sponge Verongia(syn: Aplysina)...

... Fujimoto S,Oku A, Tsuda K, Toyokuni S, Hiai H, Mizunoya W, Fushiki T, Holst JJ,Makino M, Tashita A, Kobara Y, Tsubamoto Y, Jinnouchi T, Jomori T, Seino Y: Inhibition of gastric inhibitory polypeptide ... enzymaticactivity. Inactivation of the miR-30 family drasticallyreduced GPDH activity at day 10 (fold reduction of 23 .9) .Interestingly, over-expression of miR-30a and miR-30dwas sufficient to ... activity at day 4 (fold induc-tion of 1.6; Figure 4b). Finally, we checked for the expres-sion of the adipogenic-induced transcripts C/EBPb,PPARg and fatty acid binding protein (FABP) 4. Thesegenes...

... revascularization in coronary patients is one of the main goals in coronary surgery, use of Mini-HLM and thus minimizing the side effects of CCPB is a desirable modern approach. In today´s economically affected ... stimulus of a generalized inflammatory state and thus having the potential to result in significant morbidity [1]. In order to decrease morbidity and mortality associated with coronary surgery, myocardial ... Bory M, Sampol J, Dignat-George F. Direct evidence of endothelial injury in acute myocardial infarction and unstable angina by demonstration of circulating endothelial cells. Blood 199 9; 295 1-8....

... from 199 9 to 2010 were identified and synthesized to compare the efficacy and safety of LVRS vs conservative medical therapy. One RCT was obtained regarding comparison of median sternotomy ... mortality in 3 months (including Fishman’s study); B: mortality in 6 months; C: mortality in 12 months(including Fishman’s study); D mortality in 3 months (excluding Fishman’s study); E mortality ... reducing the degree of hyperinflation, therefore improving mechanics of diaphragm and chest wall, (3) reducing heterogeneity, (4)increasing work of breathing, and improving of alveolar gas exchange...

... preliminarily selected Cys2 59, Cys261 and Cys262 in the C-terminal region of the enzyme and prepared two mutants, C259S ⁄ C261S and C262S. Weselected Cys2 59 and Cys261, as a disulfide (Cys246-Cys248) ... is localized in the C-terminal region and that the C-terminal peptide is necessary for the integrity of the active site. More-over, the binding of 5¢-deoxy-5¢-methylthioadenosine induces a conforma-tional ... Ala225 and Thr2 29, respectively, and the absence of Leu2 79 could contribute to modify the hydrophobicenvironment near the 5¢-position of the purine nucleo-side allowing SsMTAPII to bind adenosine.The...

... on the differentphosphorylation of MAP kinase.Effects of MAP kinase inhibitors on DNA synthesis in HCS-2/8 and RGC cells To investigate the signal pathways for proliferation of chondrocytes induced ... Dudley, D.T. & Saltiel, A.R.( 199 5) PD 098 0 59 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo. J. Biol.Chem. 270, 274 89 27 494 .36. ... hand, the p38 MAPK inhibitor, SB203580, completely inhibited the CTGF/Hcs24-induced synthesis of proteoglycans in HCS-2/8 cells and RGC cells but the MEK1/2 inhibitor,PD 098 0 59, did not. These...

... significantly affects adsorption of the enzyme on crystalline cellulose,but has no effect on the hydrolysis of xyloglucan, indicating that the domain may contribute to the localization of the enzyme. ... domain and the C-terminal domain belonging to the carbohydrate-binding module (CBM) family 1 [21,22]. In the presentstudy, we have heterologously expressed the cDNAencoding Xgh74B in the methylotrophic ... suggesting that the degradation processinvolved random hydrolysis of b-1,4-linkages in the xyloglucan polymer chain. On the other hand, the de-gradation pattern of Xgh74B was rather similar to...

... 2000),173–1 79. 352sibility and authority in defining and approving the use of new words. The process of defining a newword is manual and time-consuming as each wordmust be approved by a working group of ... eachstring, S1 to SN and records the longest string pat-tern which occur in both Sj and the other string in the set. On the other hand, the most fre-quent pattern-matching strategy iterates ... provides sup-porting algorithms and tools for automati-cally identifying and extracting unknownwords from Web pages of given URLs. The system yields the result of unknown-word candidates which...