... interplay between several systemic factors andan array of local factors such as cytokines, inflammatory medi-ators and growth factors. As well as IL-1β, TNFα, and IL-6,local inflammatory mediators, ... RANKL.forRANKL.rev5'-TGGAAGGCTCATGGTTGGAT-3'5'-CATTGATGGTGAGGTGTGCAA-3'COX-2, cyclooxygenase-2; iNOS, inducible nitric oxide synthase; OPG, osteoprotegerin; RANK, receptor activator of nuclear factor-κB; RANKL, receptor activator of nuclear factor-κB ligand.Arthritis ... rTNFα or rIL-10 in PBScontaining 10% (v/v) fetal bovine serum. The amounts of cytokine in the serum were determined by extrapolation of absorbance to the standard curve. The intra-assay and inter-assay...