Ứng dụng sinh học phân tử trong chẩn đoán viêm gan virus C pdf

... đư c sử dụng rộng rãi để chẩn đoán, giúp tiên lượng c ng như quyết định phương hướng điều trị viêm gan mạn do virus C, do đó khi c xét Ứng dụng sinh h c phân tử trong chẩn đoán viêm gan virus ... phân tử sau đư c ứng dụng trong chẩn đoán và điều trị viêm gan virus C. 1. C c kỹ thuật khuếch đại m c tiêu. nghiệm anti HCV dương tính, thì c c xét nghiệm sinh h c phân tử sau c n làm đó là ... bệnh về vi sinh vật nói chung c ng như c c bệnh lý về viêm gan virus nói riêng, nhờ đó mà c rất nhiều bệnh đư c điều trị triệt để. Ngày nay, vi c ứng dụng sinh h c phân tử trong chẩn đoán điều...

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Báo Cáo ứng dụng kỹ thuật sinh học phân tử trong chẩn đoán trước và sau sinh bênh alpha thaiassemia

... Thalassemia, chúng tôi đã tiến hành chẩn đoán trư c sinh cho 3 gia đình c tiền sử sinh con đầu m c HbH. C c sản phụ đư c tiến hành ch c ối tại thời điểm thai đư c 16-18 tuần tuổi. Hai thai nhi đư c phát ... HbH đư c tiến hành xét nghiệm di truyền x c định đột biến trên gen HbA1 và HbA2 tại Khoa Di truyền và Sinh h c phân tử BV Nhi Trung ương. ỨNG DỤNG KỸ THUẬT SINH H C PHÂN TỬ TRONG CHẨN ĐOÁN ... luận: Áp dụng thành c ng kỹ thuật Multiplex-PCR, C- ARMS-PCR và giải trình tự gene trong vi c phát hiện c c đột biến trên vùng gene α globin, là c sở vững ch c cho chẩn đoán trư c sinh bệnh...

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Ứng dụng sinh học phân tử trong các bệnh lý ung thư

... ovarian cancer; PC: pancreatic cancer; PrC: prostate cancer; TC: thyroid cancer (Laurent-Puig P, Clin Cancer Res 2009) BẤT THƯỜNG PHÂN TỬ TRONG UNG THƯ THU C C CHẾ FAK VÀ SRC (Bolos V, OncoTargets ... mất ch c năng; xanh dương: tăng biểu hiện gen. BC: breast cancer; CRC: colorectal cancer; EC: endometrial cancer; GB: glioblastoma; HNC: head and neck cancer; LC: lung cancer; Me: melanoma; OC: ... CHẾ T C ĐỘNG C A PRIMA-1 1 2 3 4 5 6 7 8 9 10 PCR 1 PCR 2 PCR 3 PCR 4 PCR 5 11 12 PCR 6 PCR 7 13 14 15 16 17 PCR 8 PCR 9 PCR 10 PCR 11 18 19 20 21 22 23 24 PCR 12 PCR 13 PCR 14 PCR 15...

Tài liệu Nghiên cứu ứng dụng sinh học phân tử trong tạo dòng phục hồi phục vô công tác chọn tạo giống lúa lai 3 dòng ppt

... R100 đư c sử dụng chạy kiểm tra di truyền c c cá thể trong quần thể F2 để x c định liên kết c a chỉ thị phân tử với gen ph c hồi bất d c đ c tế bào chất. Kết quả phân tích sự đồng phân ly ... kiểu gen c a c c chỉ thị SSR cho gen ph c ph c hồi bất d c đ c tế bào chất trong quần thể F2 cho thấy 40 c thể F2 chia thành hai nhóm rõ rệt 29 c thể hữu d c và 11 c thể bất d c. Hai nhóm ... trên nhiễm s c thể số 10, với hai chỉ thị phân tử lµ RM258 và RM171 (www.gramene.org) (hình số 4). NGHIÊN C U ỨNG DỤNG SINH H C PHÂN TỬ TRONG TẠO DÒNG PH C HỒI PH C VÔ C NG T C CHỌN TẠO GIỐNG...

ỨNG DỤNG SINH HỌC PHÂN TỬ TRONG CÁC BỆNH LÝ UNG THƯ doc

... glioblastoma; HNC: head and neck cancer; LC: lung cancer; Me: melanoma; OC: ovarian cancer; PC: pancreatic cancer; PrC: prostate cancer; TC: thyroid cancer (Laurent-Puig P, Clin Cancer Res 2009) ... Trastuzumab (Herceptin) 10 Đỏ: đột biến thêm ch c năng; xanh lá: đột biến mất ch c năng; xanh dương: tăng biểu hiện gen. BC: breast cancer; CRC: colorectal cancer; EC: endometrial cancer; GB: glioblastoma; ... (Chiang AC, NEJM 2008) C C NHÓM GEN TRONG QUÁ TRÌNH UNG THƯ 38 Điện di GIẢI TRÌNH TỰ CHUỖI DNA ĐỂ X C ĐỊNH ĐỘT BIẾN GEN Tinh sch sản phẩm Giải trình tự DNA ỨNG DỤNG SINH H C PHÂN TỬ...

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ứng dụng sinh học phân tử trong bệnh lý huyết học

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Phát hiện DNA phôi thai trong máu mẹ bằng kỹ thuật sinh học phân tử nhằm chẩn đoán và đánh giá một số bệnh di truyền

... 1.3.2. Chẩn đoán thai lệch bội lẻ Chẩn đoán không can thiệp phát hiện thai lệch bội lẻ rất đư c c c nhà khoa h c quan tâm, đó là do nguy c sảy thai mà c c quy trình chẩn đoán c xâm phạm ... chuẩn [5]. 2Ở Việt Nam, cho đến nay hầu hết vẫn sử dụng c c kỹ thuật xâm phạm để chẩn đoán trư c sinh, c n c c xét nghiệm sàng l c thì chỉ sử dụng c c marker c tính đ c hiệu không cao ... như ΑFP, hCG, uE3… Chưa t c giả nào áp dụng kỹ thuật chiết tách DNA phôi thai từ huyết thanh mẹ làm tiền đề cho vi c chẩn đoán trư c sinh bằng c c kỹ thuật sinh h c phân tử. Do vậy, chúng tôi...

77
1,215
5

ứng dụng chỉ thị sinh học phân tử trong nghiên cứu bệnh lý cây trồng

... 5’-CTCGTAGACTGCGTACC CATCTGACGCATGGTTCC-5’ C u tr c của adapter MseI là 5’-GACGATGAGTCCTGAG TACTCAGGACTCAT-5’Vị trí giới hạn trên adapter đư c loại bỏ bằng c ch thay đổi c p bazo cuối c ng. Vị trí ... phân tử để chẩn đoán bệnh giúp đẩy nhanh quá trình chọn giống. trưng cho từng c thể trong loài, vi c tạo ra c c sản phẩm PCR c kích thư c kh c nhau đó c ng chính là c c Microsatellites c ... c thể). Nhờ c sự hỗ trợ c a chỉ thị phân tử (MAS) giúp c c nhà chọn giống tiết kiệm thời gian chi phí trong quá trình chọn tạo (5). Chính vì những hữu ích và tiện dụng c a chỉ thị phân tử...

14
2,488
10

Ứng dụng của sinh học phân tử trong y học pptx

... enzim chẩn đoán bệnh, kĩ thuật ch c ối chẩn đoán trƣ c khi sinh, kết hợp với c c phân tích hoá sinh nƣ c ối, chuẩn đoán bằng DNA phôi thai trong máu mẹ, PCR, real-time PCR, kỹ thuật c t đoạn ... nhau về trình tự . IV. CHẨN ĐOÁN BỆNH DI TRUYỀN1. Chẩn đoán trư c sinh  M c tiêu chính: cung c p cho c c gia đình c nguy c c c thông tin c n thiết để họ c thể lựa chọn trong quá trình mang ... ích c a chẩn đoán trư c sinh:  Tái khẳng định cho c c gia đình c nguy c yên tâm khi kết quả chẩn đoán bình thƣờng. Cung c p thông tin về nguy c đối với c c cặp vợ chồng mà nếu không c ...

69
2,152
31

Nghiên cứu ứng dụng các kỹ thuật sinh học phân tử trong phát hiện sớm và dự báo tiên lượng của ung thư thế bào gan nguyên phát trên bệnh nhân nhiễm virut viêm gan b (HBV)

... HBx-core R 5’-TTTCCCACCTTATGAGTCCA.A-3’ 21 50 HBcore F 5’-CA.AGCCTCCA.AGCTGTGCCTTGGGTGGCCTT-3’ 31 68 HBcore-A R 5’-TTCTTCTTCTAGGGGACCTGCCTCGGTCCCG-3’ 31 66 HBcore-noA R 5’-TTCTTCTTCTAGGGGACCTGCCTCGTCGTCT-3’ ... hợp đư c th c hiện sử dụng c p mồi X-F1374: 5’-CCGCTCGAGATGGCTGCTAGGCTGTACTGCC-3’ và X-R1856: 5’-CCGGGGCCCGGACATGTACAAGAGATGA-3’, HBV- nt. 1–483 (HBx-ATG = nt 1). C c mồi đư c thiết kế c đầu ... BCP F: 5’-CTGTCGTCCTCTCGCGGAAAT-3’ BCP R: 5’-CTCCACAGTAGCTCCAAATTCT-3’ Chu trình nhiệt: 950 C 5 phút 940 C 30 giây 560 C 30 giây 45 chu kỳ 720 C 45 giây 720 C 5 phút 40 C ∞ ...

254
1,124
1

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