Molecular Biology Problem Solver 42 ppsx
Molecular Biology Problem Solver 58 ppsx
... conditions, as source ofproblems, 21–22, 40–42Storage phosphor imagersdynamic range of, 442–443erasure of, 447in nucleic acid hybridization,441–448operation of, 441problems with, 447–448quantitative ... 350–353native PAGE versus, 345–348problems with, 480–481standardized gels for, 363Sealed containment rooms, inbiosafety, 117Secondary antibodies, 384problems with, 395Secondary decomposition, ... 183Tritiumautoradiography film and, 438–439as radioactive waste, 158 shielding for, 163signal strength of, 406storage phosphor imagers for, 446Troubleshooting. See ProblemsTroutman, Trevor, 49, 51Trypanosoma,...
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Molecular Biology Problem Solver 1 pdf
... MOLECULAR BIOLOGY PROBLEM SOLVER Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. GersteinCopyright © 20 01 by Wiley-Liss, Inc.ISBNs: 0-4 71- 37972-7 (Paper); 0-4 71- 22390-5 ... Permissions Department, John Wiley & Sons, Inc., 605 Third Avenue,New York, NY 10 158-0 012 , ( 212 ) 850-6 011 , fax ( 212 ) 850-6008, E-Mail:PERMREQ@WILEY.COM.This publication is designed to provide ... will provide insightand perspective to help you solve problems that go beyond the contentof this book.ixPreface MOLECULAR BIOLOGY PROBLEM SOLVER A LABORATORY GUIDEEdited byAlan S. GersteinA...
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Molecular Biology Problem Solver 2 potx
... . . . . . . . . 9 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. GersteinCopyright © 20 01 by Wiley-Liss, Inc.ISBNs: 0-471-379 72- 7 (Paper); 0-471 -22 390-5 (Electronic)nique ... Project Will DefineYou 2 Which Research Style Best Fits Your Situation? . . . . . . . . . . 2 Do You Have the Essential Resources? . . . . . . . . . . . . . . . . . . 2 Expect the Unexpected ... analternative needs to be found. A global problem is one in whichreliable data point you in a direction far removed from the original plan.Technical problems are ultimately the responsibility...
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Molecular Biology Problem Solver 3 pdf
... . . . . . . . 19 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. GersteinCopyright © 2001 by Wiley-Liss, Inc.ISBNs: 0-471 -37 972-7 (Paper); 0-471-2 239 0-5 (Electronic)Big ... . . . . . . . . . . . . . 13 Does a Company Test Every Application for a Product? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 How Well Will the Product ... . . . . . . . . . . 13 How Well Will the Product Perform? . . . . . . . . . . . . . . . . . . 13 Are Identical Products Manufactured Identically? . . . . . . . . . 14Will a Company Inform You...
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Molecular Biology Problem Solver 4 pptx
... the wrong temperature.Solving Problems by YourselfIn a perfect world, six steps will solve any problem: 1. Define the problem. What do you see?The first step in any problem- solving activity is ... nature of the problem without drawing conclusions.Understanding a problem consists of describing all the factualaspects about the problem. Do not try to determine what causedthe problem. That ... of a problem are alwayshardest for the person closest to the problem to see. For example,is a piece of equipment plugged in? This is so obvious that it is an20 Tyre and KruegerChapter 4, “How...
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Molecular Biology Problem Solver 15 ppsx
... . . . . . . . . . . 150 A Wipe Test Detected Radioactivity on the Outside of the Vial. Does This Indicate a Problem? . . . . . . . . . . . . 151 Molecular Biology Problem Solver: A Laboratory ... Requirements of Autoclaving?The minimum requirements for sterilization are 15 lb of pres-sure per square inch at 115 C for 15 minutes of sterilization time(not including the exhaust cycle). Slightly ... Reference Date Has Long Passed? . . . . . . . . . . . . . . 150 Handling Radioactive Shipments . . . . . . . . . . . . . . . . . . . . . . . . . 150 What Should You Do with the Radioactive Shipment...
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Molecular Biology Problem Solver 16 ppsx
... . . . . . . . 164 How Can You Concentrate a Radioactive Solution? . . . . . . 164 Bibliography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166 Appendix ... . . . . . . . . . . . . 161 What Can You Do to Achieve Minimum Radioactive Dose? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162 How Can You Organize ... Radionuclides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166 The information within this chapter is designed as a supple-ment, not a replacement, to the...
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Molecular Biology Problem Solver 18 ppsx
... . . . . . . 185 Silica Resin-Based Strategies . . . . . . . . . . . . . . . . . . . . . . . . . . . 186 Isolation from Electrophoresis Gels . . . . . . . . . . . . . . . . . . . . 187 What Are ... DNA and subsequent ethanol washes eliminate such acontaminant. Phenol can also be problematic. If you experienceproblems with DNA purified by a phenol-based strategy, applychloroform to extract ... based on yields pub-lished for samples similar if not identical to your own.Do You Require High Molecular Weight Material?The average size of genomic DNA prepared will vary betweencommercial...
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Molecular Biology Problem Solver 22 ppsx
... totalRNA isolation. In this section we address problems presented bydifficult tissues and offer troubleshooting techniques to help over-come these problems. A separate section will discuss the ... RNA-binding filters. Increasing the ratio of sample mass: volume of lysisbuffer can help alleviate this problem in filter-based isolations.Multiple acid–phenol extractions can be done to ensure that mostof ... prove quite refractory to isolatinggood-quality RNA due to the difficulty of lysing them. Another problem with bacteria is the short half-life of most bacterial mes-sages. Lysis can be facilitated...
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Molecular Biology Problem Solver 31 ppsx
... electrophoresis may not allow you to reach the conclusion thatthere are two different mRNA forms if their molecular weightsare similar.You might require a separation technique with greaterresolving power.Suppose ... primerdegradation by the 3¢–5¢ exonuclease activity (de Noronha andMullins, 1992 and Skerra, 1992).The problem of reduced yield canbe corrected by including an enzyme with strong proofreadingactivity ... activity canpotentially reduce yield, especially with large PCR products.As discussed above, this problem can be avoided by utilizing acombination of polymerases that possess and lack strong proof-reading...
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Molecular Biology Problem Solver 50 ppsx
... 521Planning the Baculovirus Experiment . . . . . . . . . . . . . . . . . . . . . 521 Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. GersteinCopyright © 2001 by Wiley-Liss, ... an Appropriate Expression Host . . . . . . . . . . . . . . . 501 Selecting an Appropriate Expression Vector . . . . . . . . . . . . . 506 Implementing the Eukaryotic Expression Experiment . . . ... Production Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . 531Mutation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 531Solubility Problems...
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Molecular Biology Problem Solver 54 ppsx
... Acad.Sci. U.S.A. 95:2509–2514.Higgins, D. R., and Cregg, J. M. 1998. Pichia Protocols: Methods in Molecular Biology, vol 103. Humana Press, Totowa NJ.Hill-Perkins, M. S., and Possee, R. D. 1990. A baculovirus ... baculovirus-infected insect cells:Expression, purification and characterization. Prot. Expr. Purif. 10: 154 161.Gignac, S. M., Brauer, S., Hane, B., Quentmeier, H., and Drexler, H. G. 1991.Elimination ... expression in mammaliancells by tetracycline-responsive promoters. Proc. Nat. Acad. Sci. U.S.A.89: 5547 –5551.Graber, S. G., Figler, R. A., Kalman-Maltese,V. K., Robishaw, J. D., and Garrison,J....
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Molecular Biology Problem Solver 57 ppsx
... hybridizationexperiments, 402 Problem reduction, 2 Problem resolution, failed, 28Problems. See also Global problems;Technical problemswith acrylamide polymerization,342–343Index 557 Ink, for marking ... (piperazine diacrylamide), ascrosslinker, 338Peer-reviewed journals, 5Pelleting, 56, 57, 65improving, 66–67Pellets, problems with RNA, 220–221Peptide electrophoresis, buffersystems for, 350Percent ... centrifugation, 57 Nucleic acid-rich tissue, total RNAisolation from, 208Nucleic acids. See also DNA samples;Plasmids; RNAconstant current separation of, 352crosslinking, 422–424elution from gels, 357, ...
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