... were sacrificed in a carbon dioxide chamber.Tissue isolation and dissociationAfter bathing the animals in absolute ethanol, they weredecapitated and had the temporal bones removed andmaintained ... epithelia by transforming growth factor alpha andepidermal growth factor. Proc Natl Acad Sci USA 1995, 92:3152-3155.46. Raphael Y, Ohmura M, Kanoh N, Yagi N, Makimoto K: Prenatal maturation of endocochlear ... andOesterleshowedthroughautoradiographic techniques after tritiated thymidinelabeling that simultaneous infusion of TGFa and insulindirectly into the inner ear of adult rats stimulated DNAsynthesis...