... excess of 0.2 m phosphate buffer pH 7.5.Synthesis of the fluorescent Cbl analogue CBC-244Activation of the 5¢ hydroxyl group in the a-ribofuranosidemoiety of CNCbl was performed with help of 1,1¢-dicarbo-nyl-di-(1,2,4-triazole) ... Department of Molecular Biology, University of Aarhus, Denmark2 Department of Chemistry, University of Utah, Salt Lake City, UT, USA3 Department of Physiology and Biophysics, University of Aarhus, ... retention of Cbl.Additionally, a His residue substituted for water of H2OCbl, which added to protection of the ligandagainst reduction and coordination of other com-pounds. The structure of TC–Cbl...