application of arrayed libraries for analysis of differential gene expression
báo cáo khoa học: " Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissues" docx
Ngày tải lên :
12/08/2014, 03:20
... preparation) As another test of the veracity of this type of co-normalization and subsequent analysis, the expression values for eight genes with known nectary-enriched expression profiles were examined ... Comparison of gene expression in different nectary types Comparison of gene expression in different nectary types The number of genes displaying a two-fold or greater difference in expression ... significant (p or q > 0.05) For a more in-depth analysis of genes displaying the largest differences in expression, lists of genes displaying fivefold or greater differences in expression level between...
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Báo cáo khoa học: Differential gene expression profiles of red and green forms of Perilla frutescens leading to comprehensive identification of anthocyanin biosynthetic genes doc
Ngày tải lên :
23/03/2014, 07:20
... crude drug Differential gene expression analysis of the two forms by cDNAdifferential display led to the identification of several genes involved in biosynthetic reactions (structural genes) and ... regulation of the expression of biosynthetic genes (regulatory genes) [4,9–11] However, the coverage of gene expression profile by cDNA -differential display seems still incomplete, given the lack of a ... pictures of differential gene expression profiling between the anthocyanin-producing red form and the nonproducing green form of P frutescens Among the differentially expressed genes, two new genes...
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Báo cáo hóa học: " Differential Gene Expression to Investigate the Effects of Low-level Electrochemical Currents on Bacillus subtilis" doc
Ngày tải lên :
20/06/2014, 23:20
... general knowledge that biofilms are highly tolerant to antibiotics, treatment of B subtilis BE1500 biofilms with 1000 µg/mL ampicillin for 15 only killed 59% of biofilm cells; while the MIC for ... follow the kinetics of viability and gene expression over time In summary, we conducted a detailed study of the effects of weak DC on viability, gene expression and morphology of B subtilis The ... influence of electrochemical products on gene expression In addition to grouping genes induced and repressed under each condition, cluster analysis was also performed to identify the genes induced...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
Ngày tải lên :
09/08/2014, 01:23
... that the stimulated PBMC are a source of mRNA for the target genes of interest The expression of other relevant genes, including IL-4, IL-10 and IFN-γ, generated satisfactory standard curves ... for each tissue and then the mean coefficient of variation was determined for each target gene Data are presented as cell equivalents of expression and GAPDH normalized expression (relative expression) ... the CE values of the target genes are normalized to a reference gene using the CE for GAPDH The RE compared with GAPDH is determined by dividing the CE of the target gene by the CE for GAPDH As...
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Báo cáo y học: "Differential gene expression of bone anabolic factors and trabecular bone architectural changes in the proximal femoral shaft of primary hip osteoarthritis patients" pptx
Ngày tải lên :
09/08/2014, 08:23
... between gene expression and bone architecture were explored The results of the data are indicative of the generalised skeletal distribution of primary OA pathology and suggest that gene expression ... factor gene expressions were similar between OA and control individuals The mean and median values for the growth factors, as well as the differential gene expression of the α1 and α2 chain of collagen ... use of OCN as a marker of bone formation [35,36] Previous studies report increased OCN levels in OA serum, protein, and mRNA gene studies [14,16] Our finding of differential OCN mRNA gene expression...
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Báo cáo y học: "Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration" ppt
Ngày tải lên :
09/08/2014, 14:20
... include the effect of TNF-α on matrix and MMP gene expression in normal and degenerate NP cells as well as the effect of IL-1β and TNF-α on cytokine gene expression and the expression of the associated ... difference in the basal gene expression levels between normal and degenerate cells for any of the other matrix genes examined (data not shown) The addition of recombinant TNF-α for 48 hours significantly ... degenerate NP cells Effect of tumour necrosis factor-alpha on matrix gene expression The basal gene expression for the collagen type I gene was significantly higher in the degenerate cells than in...
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Báo cáo y học: "An atlas of bovine gene expression reveals novel distinctive tissue characteristics and evidence for improving genome annotation" pot
Ngày tải lên :
09/08/2014, 22:23
... classification of other tissues as well A heat map of expression of the nine mitochondrial genes in Figure provides visual context for the basis of clustering used by Simcluster in both of the Simcluster ... similarly in both profiles supports Page of 18 the existence of a coordination of expression between nuclear and mitochondrial genes Localization of sense and antisense tags within gene models and ... abundance (TPM) of sense tags was 11.5 times that of antisense tags An analysis of the behavior of the number of unique sense and antisense tag sequences within loci versus the number of unique loci...
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Báo cáo y học: "Differential gene expression in anatomical compartments of the human eye" pptx
Ngày tải lên :
14/08/2014, 14:22
... results of the disease gene analysis, including the list of candidate genes for each disease genes for for disease Results of diseases genetic intervals The list intervals used in and Compartment gene ... aspects of ocular pathology deposited research One direct application of the gene expression patterns we have defined is the identification of candidate genes for genetic diseases that differentially ... similarly Many of the array elements encode uncharacterized genes and only a subset of named genes is shown (b) Macula versus peripheral retina gene expression Using the statistical analysis of microarrays...
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IDENTIFICATION OF A MINIMAL CIS-ELEMENT AND COGNATE TRANS-FACTORS REQUIRED FOR THE REGULATION OF RAC2 GENE EXPRESSION DURING K562 CELL DIFFERENTIATION
Ngày tải lên :
24/08/2014, 12:22
... factor is required for Rac2 gene expression upon PMA stimulation of K562 cells 102 IV Changes in chromatin structure are required for the induction of Rac2 gene expression upon PMA ... regulation of genes Genomic DNA is the ultimate template of our heredity In spite of the completion of the human genome project, many challenges remain in understanding the regulation of genetic information ... molecular mechanisms regulating Rac2 gene expression during cell differentiation and identification of a minimal cis-element required for the induction of Rac2 gene expression during K562 cell differentiation...
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Analysis of silicatein gene expression and spicule formation in the demosponge amphimedon queenslandica
Ngày tải lên :
13/10/2015, 14:07
... are therefore of increasing scientific and economic value as the mechanism responsible for the formation of their biosilica structures is of potential importance for the fabrication of novel ... RNA-Seq performed on a pool of same stage individuals, revealed that all silicatein-like genes are differentially expressed throughout development, and the expression of silicatein genes occurs ... silicatein genes are expressed during A queenslandica early embryonic development, with genes being expressed exclusively in sclerocytes Analysis of gene expression levels through embryogenesis...
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Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
Ngày tải lên :
19/02/2014, 05:20
... knockdown of all Shc isoforms strongly reduced Decma-induced Erk phosphorylation Moreover, expression of silent mutants of Shc isoforms showed that only p46Shc and p52Shc rescued the effect of the ... and resulted in enhanced expression of the uPA gene and, to a lesser extent, of the MMP-9 gene (data not shown) Disruption of cell–cell adhesion by calcium chelation Loss of E-cadherin function ... Lehembre and G Christofori (Center of Biomedicine, University of Basel, Switzerland) and details will be published elsewhere Plasmids and siRNAs Construction of expression vectors for HA-tagged fulllength...
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Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx
Ngày tải lên :
07/03/2014, 00:20
... the expression of ASNS and that of leucine on CHOP (also known as GADD 153) gene expression (Table 1) Indeed, the first identification of a sequence responsive to amino acid (AARE) was performed ... activation of NF-jB DNA-binding may account in part for increased expression of the IL-8 gene [90] In addition to STAT [91], the amino acid was shown to act at the level of the inhibitor of kappa ... on ASS gene expression via a decrease in the nuclear amount of NF-jB [44] This illustrates that glutamine may regulate expression of the same gene via different pathways as a function of cell...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
Ngày tải lên :
17/03/2014, 10:20
... N (1999) Genetic analysis of nif regulatory genes by utilizing the yeast two-hybrid system detected formation of a NifL-NifA complex that is implicated in regulated expression of nif genes J Bacteriol ... gene of Escherichia coli and metal ion requirement for FNR-regulated gene expression Mol Microbiol 3, 601–608 Meng, W., Green, J & Guest, J.R (1997) FNR-dependent repression of ndh gene expression ... different regulation of the nuo-operon in K pneumoniae M5a1 Based on preliminary sequence analysis of the promoter upstream regions of the K pneumoniae nuoA gene and determination of the NADH oxidation...
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Báo cáo Y học: Prediction of temporal gene expression Metabolic optimization by re-distribution of enzyme activities ppt
Ngày tải lên :
17/03/2014, 10:20
... values for NADH and ATP, respectively The time courses of the metabolite concentrations in Fig indicate that reprogramming of gene expression under stress conditions allows for homeostasis of metabolites ... (shortterm) goal of genetic regulation [23,24] Dealing with the evolutionary optimization of gene expression in mathematical terms requires substantial simplications in view of the complexity of cellular ... microarrays for genome wide parallel genetic and gene expression analysis Proc Natl Acad Sci USA 94, 1305713062 11 Heinrich, R., Schuster, S & Holzhutter, H.G (1991) Mathematical analysis of enzymic...
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Báo cáo khoa học: Ablation of Sax2 gene expression prevents diet-induced obesity docx
Ngày tải lên :
28/03/2014, 23:20
... affected by lack of Sax2 gene expression A B C D E F Fig Analysis of blood glucose, insulin and leptin levels of female Sax2+ ⁄ + and Sax2) ⁄ ) animals (A) Blood glucose levels of Sax2+ ⁄ + and ... the loss of Sax2 expression Discussion Sax2, also called Nkx1.1, is a homeobox gene of the Nkx1 gene family located on chromosome of the mouse genome The human homolog is located on Sax2 expression ... 2010 FEBS 373 Sax2 expression required for diet-induced obesity R Simon et al A B C D Fig Sax2 expression is required for diet-induced obesity (A–D) Determination of weight gain of single housed...
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Báo cáo hóa học: " Inhibition of cytokine gene expression and induction of chemokine genes in non-lymphatic cells infected with SARS coronavirus" doc
Ngày tải lên :
20/06/2014, 01:20
... picture of SARS-CoV-induced cytokines In some cases Growth of SARS-CoV in different cell lines Vero cells, which are standard for growth of SARS-CoV [30,31], lack type I IFN genes [32,33] and therefore ... systems for studying the influence of SARS- CoV on the immune system-independent induction of cytokines Interferon genes and their antiviral effectors To properly assess the cytokine profile of SARS-CoV ... Interferon-stimulated genes Interferon-stimulated genes RNA samples of Caco-2 cells (A) and HEK 293 cells (B) described in Fig were investigated by RT-PCR for the presence of ISG56 and MxA mRNAs As for IFN-α...
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Báo cáo sinh học: "The functional landscape of mouse gene expression" pdf
Ngày tải lên :
06/08/2014, 18:21
... similar overall gene expression profiles For example, all components of the nervous system featured higher expression of a common subset of transcripts, as did all components of the lower digestive ... characterization of gene functions in mammals, as well as for dissecting the mammalian transcriptional regulatory hierarchy Results Expression analysis of mouse XM gene sequences In order to generate ... functional categories For example, Figure shows the expression of individual genes in 17 categories that exemplify ways in which gene expression relates to gene function (similar diagrams for all GO-BP...
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Báo cáo sinh học: "Conservation of core gene expression in vertebrate tissues" pdf
Ngày tải lên :
06/08/2014, 19:20
... conservation of expression of individual genes We used two conceptually simple measures intended to capture different aspects of conservation of expression The first asks how often specific gene expression ... opportunity for both neutral drift and positive selection, would allow assessment of the degree of conservation of tissue gene expression among all vertebrates, and a comparison of the conservation of expression ... the conservation of gene expression in these tissues extends beyond the base of vertebrates; coexpression of neuronal genes, for example, is observed as far as nematodes [54] Genes expressed in...
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Báo cáo y học: "Comparison of marker gene expression in chondrocytes from patients receiving autologous chondrocyte transplantation versus osteoarthritis patients" pptx
Ngày tải lên :
09/08/2014, 10:20
... currently used for ACT and are able to generate hyaline cartilage Materials and methods Molecular strategies for monitoring the gene expression patterns of chondrocytes destined for ACT were developed ... reaction was stopped by washing with PBS Results Analysis of gene expression patterns in chondrocytes ex vivo In the first set of measurements, gene expression patterns were investigated in cells ... be able to cure any type of cartilage damage, regardless of pathology, degeneration of tissue, health status and age of patient [13] In a recent study, regeneration of cartilaginous tissue was...
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Báo cáo y học: " Large scale comparison of global gene expression patterns in human and mous" pot
Ngày tải lên :
09/08/2014, 22:23
... file 7) Identification of expression correlation between orthologous genes of different species Cross-platform comparison of gene expression data is always a challenge Even for the same tissue type, ... of human and mouse, while the same tissue of the same species displays the highest correlation of gene expression levels, the same tissue of different species often has a higher correlation of ... orthology a good fraction of the most variable genes (tissue-specific genes) and the most constant genes (housekeeping genes); the level of sharing is as strong as the level of human genes co-vary between...
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