Báo cáo khoa học: Cloning of type 1 cannabinoid receptor in Rana esculenta reveals differences between genomic sequence and cDNA pot

... Journal compilation ª 2007 FEBS Cloning of type 1 cannabinoid receptor in Rana esculenta reveals differences between genomic sequence and cDNA Rosaria Meccariello 1 , Rosanna Chianese2, Gilda ... amino acid sequence of R. esculenta (FCNR1) was compared withthose of known CNR1s, revealing an amino acid iden-tity ranging from 61. 9% to 88 .1% among vertebrates and of 21. 5% in C. intestinalis ... human,responsible for cnr1a and cnr1b splice forms, are notconserved in frog. Interestingly, comparison between genomic DNA and cDNA, both obtained from frogFig. 1. (Continued). Cloning of cnr1 in frog R....

Báo cáo khoa học: Cloning of two melanocortin (MC) receptors in spiny dogfish MC3 receptor in cartilaginous fish shows high affinity to ACTH-derived peptides while it has lower preference toc-MSH ppt

... Genetics 15 1, 15 47 15 57.Ó FEBS 2004 MC3 and MC5 receptors of spiny dogfish (Eur. J. Biochem. 2 71) 43295. Chhajlani, V. (19 96) Distribution of cDNA for melanocortin receptor subtypes in human ... olfactory bulb.Ó FEBS 2004 MC3 and MC5 receptors of spiny dogfish (Eur. J. Biochem. 2 71) 43 31 Cloning of two melanocortin (MC) receptors in spiny dogfishMC3 receptor in cartilaginous fish shows high affinity ... haracterization, and brain mapping of the melanocortin 4 receptor in the goldfish:involvement in the control of food intake. Endocrinology 14 4,2336–2349. 19 . Strader, A.D., Schioth, H.B. & Buntin, J.D....

Báo cáo khoa học: Distribution of the lipolysis stimulated receptor in adult and embryonic murine tissues and lethality of LSR–/– pdf

... of embryosNo. of littersLSR mutants+/+ +/– –/–C57BL/6 E10.5 10 1 3 6 1 C57BL/6 E12.5 7 1 1 4 2C57BL6/ )12 9/Ola E12.5 25 3 4 14 7C57BL/6 E14.5 10 1 3 7 0C57BL/6 E14.5 8 1 3 3 2aC57BL/6 12 9/Ola E14.5 ... exon 2, intron 1, exon 1 and its 5¢-ﬂankingnoncoding sequence, and 2.7 kb of sequence containingthe 3 ¢-part of exon 5 and 3¢-ﬂanking intron 5 s equence,respectively. These were i nserted into ... fol-lowing formula: 2)dCT· 10 6. E mbryo stages (days post-coitum.): E7,E 11, E15, E17.mRNAAgeE7 E 11 E15 E17AdultLiverLSR 13 10 5680 719 0 7 810 67 500LDLR 21 900 2880 4780 2560 15 400SRBI...

Báo cáo khoa học: Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling pdf

... above and Interaction of ultraspiracle and ecdysone receptor in hormone signaling F. Fang et al. 15 86 FEBS Journal 272 (2005) 15 77 15 89 ª 2005 FEBSa construct containing a single motif in the ... by components of Sf9nuclear extracts. Binding conditions for recombinant dUSPwere 1. 0 lg of dUSP, 1 lg dIdC, 3 lLof5· binding buffer (1 :10 mm of Tris ⁄ HCl, pH 7.5, 33 mm KCl, 1 mmMgCl2, ... structure of the ultraspiracle ligand-binding domain reveals a nuclear receptor locked in aninactive conformation. Proc Natl Acad Sci, USA 98, 15 49 15 54.47 Robinow S & White K (19 91) Characterization...

Báo cáo khoa học: Supramolecular calsequestrin complex Protein–protein interactions in chronic low-frequency stimulated muscle, postnatal development and ageing pot

... TRI and the CSQ-binding elementJUN, show decreased protein–protein interactions duringﬁber type shifting and the ageing process. Reduced proteincoupling between the major elements regulating ... °C in the presence of a protease inhibitor cocktail(0.2 mMPefabloc, 1. 4 lMpepstatin A, 0.3 lME-64, 1 lMleupeptin, 1 mMEDTA, and 0.5 lMsoybean trypsininhibitor) [19 ]. Using bovine ... latterﬁnding agrees with the stimulation-induced reduction in thefast isoforms of CSQ, TRI and the RyR1 (Fig. 3E,G,H).The disproportionate weakening of immuno labeling of the RyR1 band in stimulated...

Báo cáo khoa học: Promoters of type I interferon genes from Atlantic salmon contain two main regulatory regions docx

... 1 135 13 5 3Intron 1 294 294 4Exon 2 75 75 1 Intron 2 13 0 13 0 3Exon 3 15 0 15 0 3Intron 3 19 99 213 6 52Exon 4 78 78 2Intron 4 338 3 31 12Exon 5 90 90 23¢-UTR 46b236 1 aPutative 5¢-UTR of ... gene(Fig. 2A). From the A1 gene the following constructswere made: pA1( )13 5), pA1()202) and pA1()333)containing only PR-I; and pA1()747) and pA1( )12 81) containing both PR-I and PR-II. From the ... to induce IFNTable 1. Comparison of the Atlantic salmon genomic A1 and A2IFN sequences. Sequence comparedwith SasaIFN-a1Number of nucleotidesA1 A2 Differences Upstream region 12 81 114 1 2065¢-UTR...

Báo cáo khoa học: Structures of type B ribose 5-phosphate isomerase from Trypanosoma cruzi shed light on the determinants of sugar speciﬁcity in the structural family ppt

... 24.7 1. 9 (2.00 1. 90) 30.0–2 .1 (2. 21 2 .10 ) 29.4–2 .15 (2.27–2 .15 )Number of reﬂections measured 14 1 283 455 063 2 91 2 91 108 089 13 1 784Number of unique reﬂections 28 418 79 637 63 050 23 17 5 20 ... Kim,J.H. Jung and M.K. Hong(unpublished data)EcRpiB 1NN4 Pi 15 0 255 0.9 43 14 90 2940, 13 20a [10 ]2VVR –b 14 9 264 0.9 42 [13 ]TmRpiB 1O1X MPD 15 5 250 0.94 45 16 11 1225 [19 ]MtRpiB 1USL Pi 17 0 262 ... [13 ]SpRpiB 2PPW SO4 216 16 8 1. 17 28 17 11 1890 R. Wu, R. Zhang,J. Abdullah, and A. Joachimiak(unpublished data)NaRpiB 3C5Y – 2 31 178 1. 11 23 18 73 19 24 Joint Center forStructural Genomics(unpublished...

Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot

... N229Q, n ¼ 11 ) than forglycosylated Kv3 .1 (dV is 8 .1 ± 0.4 mV for wild -type Kv3 .1, n ¼ 13 ). A range of values for Vm0.5from 10 mV to 18 mV, and for dV from 8 mV to 11 mV, of wild -type Kv3 .1 have ... immunobands corresponded tothe two faint bands of wild -type Kv3 .1 or the lowestfaint band, respectively. These results indicate that themost prominent band of wild -type Kv3 .1 expressed in insect ... (19 94) Glycosylation of Shaker potassium channelprotein in insect cell culture and in Xenopus oocytes.Biochemistry 33, 5607–5 613 . 41 Deal KK, Lovinger DM & Tamkun MM (19 94) Thebrain Kv1.1...

Báo cáo khoa học: Induction of PAI-1 expression by tumor necrosis factor a in endothelial cells is mediated by its responsive element located in the 4G/5G site ppt

... binding site in the PAI -1 promoter is responsible for IL -1- induced PAI -1 expression and this element is involved in the control of plasma levels of PAI -1 [24]. Thus, multiple cyto-kines and infectious ... 2September 2005, accepted 16 September2005)doi :10 .11 11/ j .17 42-4658.2005.04979.xPlasminogen activator inhibitor type 1 (PAI -1) is induced by many proin-ﬂammatory and pro-oxidant factors. Among ... Thymosin beta 4 induces thesynthesis of plasminogen activator inhibitor 1 in cul-tured endothelial cells and increases its extracellularexpression. Blood 10 3, 13 19 13 24. 17 Hou B, Eren M, Painter...

Báo cáo khoa học: Replacement of helix 1¢ enhances the lipid binding activity of apoE3 N-terminal domain pot

... ⁄ Picontaining 1 mgÆmL )1 fatty acid-freealbumin (FAFA), then incubated with DMEM containing1mgÆmL )1 FAFA, 2 lgÆmL )1 125I-labeled LDL and differ-ent amounts of receptor binding competitor ... presence of chaotropic agents, modulates the lipid binding activ-ity of apoE3-NT [13 ,14 ]. In the present study weemployed a protein engineering approach, removinghelix 1 and replacing it with a sequence ... apolipoproteins [15 ,16 ], we hypothesized thatresidues comprising a short connector helix in apoE3-NT function in recognition or initiation of lipid bind-ing, leading to helix bundle opening and formationof...

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