Báo cáo khoa học: Par j 1 and Par j 2, the two major allergens in Parietaria judaica, bind preferentially to monoacylated negative lipids potx

... Parietaria allergens behave as ns-LTPsFEBS Journal 276 (2009) 17 62 17 75 ª 2009 The Authors Journal compilation ª 2009 FEBS 17 75 Par j 1 and Par j 2, the two major allergens in Parietaria judaica, bind ... 01 319 1E-mail: gbbviria@lg.ehu.es(Received 5 November 2008, revised5 January 2009, accepted 19 January 2009)doi :10 .11 11 /j. 17 42-4658.2009.06 911 .x Par j 1 and Par j 2 proteins are the two major ... of the mostcommon causes of pollinosis [24]. The two major aller-gens of P. judaica, Par j 1 and Par j 2, have beencloned and sequenced, and their recombinant counter-parts were able to induce...

Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx

... explainedby their competitive inhibition at the ribosome-bindingsite [32].Together, the M and C domains of human eRF1, in the absence of the N domain, are able to bind to the mammalian ribosome and induce ... )35Gln185 )90 ± 23 13 5 ± 7 )11 0 ± 17 )75 13 5 )60Ser186 )68 ± 5 14 8 ± 4 0 ± 11 0 )73 15 0bAla187 )64 ± 1 ) 41 ± 2 )64 )42Leu188 )64 ± 1 )42 ± 1 )11 0 ± 23 )64 )42ba The mean value in the family ... RNA- and DNA-binding proteins [46–48], and may facilitate eas-ier positional rearrangement of the protein during the docking to the binding site on the ribosome or otherligands. Strikingly, the...

Báo cáo khoa học: nNOS inhibition, antimicrobial and anticancer activity of the amphibian skin peptide, citropin 1.1 and synthetic modiﬁcations pot

... c-CH3 1. 10, 1. 00 66.0 17 6.7Ala10 8.83 4.02 1. 53 54 .1 178.6Ser 11 7.83 4 .17 4 .12 , 4.03 60.7 17 4.7Val12 7. 91 3.85 2. 41 c-CH3 1. 13, 1. 02 64.8 17 6.9Ile13 8.28 3. 81 2. 01 c-CH2 1. 73, 1. 34 63.5 17 7.2c-CH30.99d-CH30.88Lys14 ... multocidaa 10 0 10 0 10 0 10 0 10 0 10 0 10 0 10 0 10 0 10 0Staphyloccus aureus 25 25 10 0 25 10 0 25 10 0 25 10 0 6 12 25 25 25 25 50 25 50 50Staphylococcus epidermidis 12 12 10 0 12 10 0 25 50 10 0 25 10 0 12 6 6 12 ... Citropin 1. 1 and synthetic analogues [MIC values (lgÆmL )1 )]. The absence of a ﬁgure means the activity is> ;10 0 lgÆmL )1 . For error range see Methods. 1. 1 1. 1D 2 3 4 7 8 9 10 11 12 13 14 15 16 ...

Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modiﬁed conotoxin, reveals that the glycan structure is a-D-Gal-(1ﬁ3)-a-D-GalNAc pot

... doi :10 .11 11 /j. 14 32 -10 33.2004.04464.xtotal, nine of the 13 residues in tx5a are modified, making itone of the most highly modified gene products identified to date. The native peptide was purified to ... monitoring to check the efficiency of the coupling reactions. In order to scale up t he automatedsynthesis to the same level as the manual synthesis(280 lmol) we carried out nine separate automated ... following alkaline hydrolysis [11 ,12 ]. The material in the major Gla-containing peak wasfurther purified on a reverse-phase column (HyCrom C 18 ,5 l ;10 · 250 mm) in 0 .1% trifluoroacetic acid and elutedwith...

Tài liệu Báo cáo khoa học: Oxidized elaﬁn and trappin poorly inhibit the elastolytic activity of neutrophil elastase and proteinase 3 pdf

... elafin and trappinElafin and the inhibitory domain of trappin each have two methionine residues (M25 and M 51 for elafin, and M63 and M89 for trappin). M25 and M63 are the P 1 ¢residues of the inhibitors’ ... sequencehomologous to a putative transglutaminase substratemotif. The trappin molecule may therefore be cova-lently attached to other proteins [11 ]. These inhibitorsare also antimicrobial [12 ,13 ] and thus participate ... to a half-life of dissociation of 36 10 5 min [15 ]. The oxidation of elafin and trappin down-shifts the half-life of there complexes with NE to % 1. 3– 1. 8 min. On the other hand, the oxidized inhibitor–Pr3complexes...

Báo cáo khoa học: Substrate speciﬁcity and transport mode of the proton-dependent amino acid transporter mPAT2 potx

... alanine, serine and proline but also osmolytes such as sarcosine and betaine, and the D-enantiomers of serine and alanine. The apparent afﬁnities of PAT1 substrates are mainly in the range of 2 15 mM [1, 2,4 ,6,9]. ... glycine and alanine were a ble to induce comparableinward currents in oocytes expressing PAT2 with a h ighafﬁnity interaction ( Fig. 1A and Table 1) . T he elongationof the side chain by just ... side ch ain w ith c orrespondingamino acid residues in the binding pocket. Whether otherproline derivates also serve as substrates of PAT2, asrecently shown for its paralog PAT1 [11 ], is p...

Báo cáo khoa học: Connection of transport and sensing by UhpC, the sensor for external glucose-6-phosphate in Escherichia coli ppt

... 14 5 75 93 81 932R 318 C 45 14 5 81 179 ConstitutiveaR 318 A 73 15 2 63 2 41 ConstitutiveaR 318 K 15 0 10 6 67 15 8 ConstitutiveaR437C 11 5 10 0 12 8 78 15 .2Salt bridgeT382D/V385K 16 0 15 0 10 15 00 n.m.Loop ... 63 11 0 10 0 11 0 3.8Arginine mutantsR46C 13 5 10 5 22 480 n.m.R149C 30 15 4 39 395 2646R152C 23 30 13 2 31 0.86R152A 46 9 37 24 3.2R152K 82 61 9 678 ConstitutiveaR204C 33 31 32 97 7.3R266C 14 5 ... mutations of the UhpC protein on both the transport and sensingfunctions of this molecule. We mutated arginine residuesas these are known to be involved in binding of anions to proteins [18 ] and as...

Báo cáo khoa học: Dual mitochondrial localization and different roles of the reversible reaction of mammalian ferrochelatase ppt

... corresponding to the two ferrochelataseactivities (Fig. 1B), were examined in the mitochondria and cytosol. The activity of cytochrome c oxidase, aninner membrane protein, was only found in the mito-chondrial ... iscleaved during the processing into the inner membraneof mitochondria [1] . Because ferrochelatase in the outer membrane has a molecular mass similar to thatof the enzyme in the inner membrane, the ... May 2009, revised 18 June2009, accepted 25 July 2009)doi :10 .11 11 /j. 17 42-4658.2009.07248.xFerrochelatase catalyzes the insertion of ferrous ions into protoporphyrinIX to produce heme. Previously,...

Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunﬂower KNOX protein HAKN1 with DNA ppt

... the footprint. Letters to the right of eachpanel indicate the DNA sequence (5¢ end in the upper part) of the corresponding strand in this region. In the lower part, the sequence of the binding ... family.ResultsExpression and DNA binding analysis of the HAKN1 homeodomain The homeodomain of the KNOX transcription factorHAKN1 was expressed in Escherichia coli as a fusionwith the maltose binding protein using ... con-tains an adenine, known to interact with Asn 51, ABFig. 4. Hydroxyl radical footprinting of I50S–HAKN1 (A) and I50K–HAKN1 (B) bound to their preferred binding sites. After binding and hydro-xyl...

Báo cáo khoa học: Phosphatidylserine induces functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 docx

... Ala 116 and Ala 118 within the C-terminal a3-helix of the PHdomain are unchanged. These signals are indifferent to the presence of the membranes, probably because the positions of Ala 116 and Ala 118 ... to the chemical shift and line shape of the peak representing Ala 112 in the PHdomain bound to IP3 in solution. The matching ofchemical shifts of Ala88 and Ala 112 with those of the PH domain–IP3complex ... afﬁnityfor binding to the membrane containing 20% PS wasrestored in the presence of 25 mm NaCl. Further addi-tion of NaCl up to 10 0 mm did not induce a furtherincrease in Kd. The differences in Kdvalues...

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