... glabra GgbAS1 b-amyrin synthase [6], was prepared byPCR using GgbAS1 as a template, Taq DNA polymerase(Takara Shuzo, Kyoto, Japan), the primers 50-GAAGCATATCCACTATGAAGATGA-30 and 50-TGAATACTCCCGTGATTTCCTGTTG-30, ... Noboru Hiraoka2, Yasumasa Ikeshiro2, Kazufumi Yazaki3,Shigeo Tanaka4, Tetsuo Kushiro5, Masaaki Shibuya5 and Yutaka Ebizuka51Gifu Pharmaceutical University, Japan;2Niigata College ... from 10-day-old-cultured Luffacells by guanidine thiocyanate/hot phenol extraction [24].Poly (A) -rich RNAwas purified by an mRNA purification kit(Pharmacia), and a Luffa cDNA library was constructedusing...