Báo cáo Y học: Human bile salt-stimulated lipase has a high frequency of size variation due to a hypervariable region in exon 11 pot
Báo cáo Y học: Human bile salt-stimulated lipase has a high frequency of size variation due to a hypervariable region in exon 11 pot
... Human bile salt-stimulated lipase has a high frequency of size variation due to a hypervariable region in exon 11 Susanne Lindquist1, Lars BlaÈ ckberg2and Olle Hernell1Departments of 1Clinical ... protein is, however,abundantly glycosylated and the apparent molecular masson SDS/PAGE has been estimated to 120±140 kDa [11, 12]. Human BSSL has a unique primary structure ascompared to other ... fetal pancreas and in pancreatic tumoralcell lines [35,36]. FAPP and BSSL are structurally closelyrelated, but are distinguished by a monoclonal antibodydirected towards a fucosylated epitope,...
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Báo cáo y học: "Human toxoplasmosis and the role of veterinary clinicians"
... Ivyspring International Publisher. All rights reserved Short Communication Human toxoplasmosis and the role of veterinary clinicians Laura Kramer Department of Animal Health- Veterinary Parasitology ... “educational” challenges for small animal clini-cians. Veterinarians are often faced with the need to explain to their clients that it is not necessary, for example, to give away one’s cat during ... box hygiene is the main precaution in preventing transmission of toxoplasmosis from pet cats [1]. A Toxoplasma-infected cat that is shedding the parasite in its feces (approximately 2% of the...
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Tài liệu Báo cáo Y học: Human and Drosophila UDP-galactose transporters transport UDP-N-acetylgalactosamine in addition to UDP-galactose doc
... 2002 Human andDrosophilaUDP-galactose transporters transportUDP-N-acetylgalactosamine in addition to UDP-galactoseHiroaki Segawa*, Masao Kawakita and Nobuhiro IshidaDepartment of Physiological ... vulval epithelial invagin-ation. Proc. Natl Acad. Sci. USA 96, 967±973.10. Miura, N., Ishida, N., Hoshino, M., Yamauchi, M., Hara, T.,Ayusawa, D. & Kawakita, M. (1996) Human UDP-galactosetranslocator: ... contain galactose andN-acetylgalactosamine residues are involved in the winglesssignaling [25]. The DmUGT protein was localized in theGolgiregionwhenthecDNAwasexpressedinLec2andLec8cells, and...
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Báo cáo Y học: Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A ppt
... ZlibHisStop-1: 5¢-TCGACCCATCATCATCATCATCATTAATAAGTCGAC-3¢ and ZlibHis-Stop-2: 5¢-TCGACGTCGACTTATTAATGATGATGATGATGATGATGGG-3¢ encoding a hexahistidyl sequencefollowed by a stop codon into the ... series of biosensor binding studies. To investigate if the IgA binding capability of the ZIgA1affibody could possibly be explained by any IgA bindingactivity present already in the Z domain scaffold ... Isotype specificity analysis of the ZIgA1 a body. Resultingoverlay sensorgrams from separate injections of 0.2 lMsolutions of human polyclonal IgA and IgA1,humanmyelomaIgA2and IgM,human...
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Báo cáo Y học: The cold-active lipase of Pseudomonas fragi Heterologous expression, biochemical characterization and molecular modeling pdf
... theanalysis of its model reveal other glutamate or aspartateresidues in that region. Another feature characterizing lipases is the so-calledoxyanion hole, an arrangement of amino acids which playsan ... secreted by a signal peptide-independent pathway and calls for furtherinvestigation on this subject.Enzyme activity and specificity In the standard tricaprylin hydrolysis assay, rPFL displayedhighest ... standard. Lipase activity was determined in a pH-stat assay bytitrating fatty acids released from triacylglycerols with0.01Msodium hydroxide using a 718 STAT TITRINO(Metrhom). Emulsions of...
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Báo cáo Y học: Human sprouty 4, a new ras antagonist on 5q31, interacts with the dual specificity kinase TESK1 potx
... downstream of ras at the level of Raf/MAP kinase [3]. Human sprouty family members areassumed to exert a function similar to inhibitors of the ras/MAP kinase signaling pathway that are induced byactivated ... assays and total lysateanalysis were adjusted to ensure identical total proteinconcentrations as determined by BCA assay (Bio-Rad).Yeast two-hybrid assayFull-length human sprouty 4 cDNA was amplified ... Myc-tagged MAP kinasewas measured by its ability to phosphorylate myelin basicprotein and was maximal 2 min after stimulation withinsulin or EGF; hspry4 inhibited MAP kinase activation byeither...
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Báo cáo y học: " Introducing the Critical Care Forum’s ongoing review of medical statistics"
... widespread use, itis important that all those involved in research or themanagement of patients have a sound grasp of at least thebasics of statistical methods. Unfortunately, in practice this ... presentationand summary of data. It is unlikely that the material covered bythis article will be entirely new to any reader but it is includedas a simple introduction to some of the ideas and philosophiesthat ... calculations;measures of disease; parametric and non-parametric tests;simple regression; and analysis of survival data. Ideally theseries will evolve to meet the needs of Critical Care readers,and you are encouraged...
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Báo cáo y học: "Mechanical complications and reconstruction strategies at the site of hip spacer implantation"
... 1). Alterna-tively, a partial (Figure 2) or normal cementation of the spacer into the femoral canal provides the advan-tage of rotational and axial stability [3]. A normal ce-mentation has ... properties of commercially available hip spacers containing a substantial stainless steel central core with experi-mental spacers containing Steinmann pins, intrame-dullary nails with two lag screws ... insufficiency or large acetabular defects, the spacer should not be implanted as a hemiarthroplasty, but rather as a total arthro-plasty, consisting of a spacer stem and a cup (Figure 3). This is also...
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Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... 94aggressive melanomas have an abundance of claudin-1 in the cytoplasm. We have shown that in- creasing claudin-1 expression in less metastatic melanoma cells caused an increase in melanoma ... F:5'-ttcctgtccccgaaaaacagactcttacccaacaccaagg-3' R:5'-ccttggtgttgggtaagagtctgtttttcggggacaggaa-3' 189-192 KTTS KXX[pS/pT] PKA A5 68G_C56 9A_ A570C_ A5 71G_C57 2A F:5'-actttgctgttcctgtccccgaaaagacgactcttacccaacaccaaggccc-3' ... nuclear claudin-1 is expressed largely in benign nevi, and early stage melanoma, but that highly me-tastatic melanoma cells tend to have increased claudin-1 in the cytoplasm and at the membrane,...
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Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx
... [26], also indicating that themidpoint potentials and cooperativity are adapted to thevarying proton path residues. To emphasize this suggestionfurther, future comparative studies on the varying ... differentcytochrome c oxidases. The heme types incorporatedbelong to the caa3-andba3-type cytochrome c oxidases,respectively. The caa3-oxidase contains analogous centralsubunits and catalytic ... the binuclear heme a 3–CuBcenter.Identification of protonable sitesAspartic and Glutamic acid side chains. The m(C¼O)mode of protonated aspartic and glutamic side chainsabsorb typically above...
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