Báo cáo khoa học: N-Terminal segment of potato virus X coat protein subunits is glycosylated and mediates formation of a bound water shell on the virion surface docx
Báo cáo khoa học: N-Terminal segment of potato virus X coat protein subunits is glycosylated and mediates formation of a bound water shell on the virion surface docx
... 2004 N-Terminal glycosylation of potato virus X protein (Eur. J. Biochem. 271) 3145 N-Terminal segment of potato virus X coat protein subunits is glycosylated and mediates formation of a bound water shell ... that the s urface-located and glycosylated N-terminal CP segments of intact PVX virions induce the formation of a columnar-type shell from bound water molecules around the virions, w hich probably ... of several absorption p eaks. Here a complex band o f valent water OH-bond vibrations is overlapped with p eaks of valent OH-bond and NH-bond vibr ations of PVX CP.Thus, to estimate the state of water...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... Mes as a buffer. Shown are stefin A and stefin B at pH 5 and variant 2 of ste-fin B and the P79S mutant of the variant atboth pH 7 and pH 5. Each panel is a plot of heat change on ligand addition ... of Bath, UKCommon features of many neurodegenerative diseasesare misfolding, aggregation and amyloid fibril forma-tion of a pathological mutant (in inherited diseases), or of a normal protein ... not only in the lysosomes and in the cyto-plasm, but also in the nucleus [19]. Lack of expression of stefin B is associated with signs of cerebellar gran-ular cell apoptosis, ataxia and myoclonus...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... purification.(b) The reaction is fast. This is advantageous for the synthesis of proteins that are sensitive to proteolyticdegradation and for high-throughput applications.(c) The reaction can be carried ... [15N]-labelledamino acids. The most abundant amino acids arelabelled in only one of the samples, while the leastabundant amino acids are labelled in up to three of the samples. The pattern of ... particular, interconver-sion between Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effect-ively be suppressed by inhibitors of transaminases and glutamine...
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Báo cáo khoa học: Selecting cells with different Alzheimer’s disease c-secretase activity using FACS Differential effect of presenilin exon 9 deletion on c- and e-cleavage doc
... (a gift from G. E. O. Muscat, University of Queensland,St Lucia, Australia) with primer 3a (5¢-GGTGATGCTGAAGAAGAAACAGTACATGAAGCTACTGTCTTCTATCG-3¢) and primer 4 (5¢-GCTCTAGAGCTTCACGGATGCATTATCGATGGGCTC-3¢). ... c-ore-secretase cleavage (Fig. 2A; lanes 3 and 4). The 31 kDa band was subjected to automated Edman degra-dation. Counting of the fractions revealed a radioactivesignal in fractions 1 and 2, suggesting ... Ab40)42), and 49 cleavage, or e cleavage, of APP. The precise cleavage mechanism is unknown and datausing PS1 dominant-negative aspartate mutations havebeen controversial. We determined the...
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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
... tem-perature on a Rigaku R-AXIS II system (Rigaku, The Woodlands, TX, USA) (at the School of Biological Sci-ences, University of Auckland, Auckland, New Zealand),whereas an in-house Rigaku R-AXIS ... than for the F14 4A mutation.Examination of the crystal structure of the F258Imutant showed it to be isomorphous with native Exgother than a small adjustment in the backbone confor-mation of ... demonstrated how the Phe-Phe arrangement acts as a clamp at the +1subsite. The terminal sugar at the )1 site showed displacement from the position of a monosaccharide analogue with interchange of water...
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Báo cáo khoa học: Methylcitrate synthase from Aspergillus fumigatus Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia ppt
... foracetate and the other for propionate. The remarkabledifference is that the overall activity for activation of acetate is always higher in A. nidulans than that for the activation of propionate ... expense of this carbon source. The main pathways involved inpropionate metabolism are that of the methylmalonyl-CoA pathway and the methylcitrate cycle. The reaction of methylmalonyl-CoA mutase leads ... aciddegradation (valine, isoleucine and methionine) at con-ditions of carbon starvation. In contrast, inhibition of naphtopyrone synthesis was only observed when pro-pionate was added to the...
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Báo cáo khoa học: Apolipoprotein E-derived antimicrobial peptide analogues with altered membrane affinity and increased potency and breadth of activity pdf
... data show that the increasedinhibitory action of apoEdpL-W and apoEdpL-Fagainst certain bacteria and viruses, including HIV, and the stronger association of apoEdpL-W peptide withmammalian ... inva-sion by a range of pathogenic organisms.Here, we dissect those features of the apoEdp pep-tide involved in its activity, and examine the activity and mechanism of action of variants of ... region of human apolipoprotein E has direct anti-infective activ-ity. J Infect Dis 193, 442–450.13 Clay MA, Anantharamaiah GM, Mistry MJ,Balasubramaniam A & Harmony JA (1995) Locali-zation...
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Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer ppt
... reportthat the occurrence of EGFR and KRAS mutations arestrictly mutually exclusive [13]. One explanation is that the KRAS–mitogen-activated protein kinase pathway is one of the downstream signaling ... development of lungadenocarcinomas and that amplification might beacquired in association with tumor progression.Relationship between EGFR and mutations of the related genes The activating mutation of ... The biological significance of such mutations and their relationship with otheractivated genes in lung cancers are also discussed.AbbreviationsALK, anaplastic lymphoma kinase; BAC, bronchioloalveolar...
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Tài liệu Báo cáo khoa học: Mitochondrial chaperone tumour necrosis factor receptor-associated protein 1 protects cardiomyocytes from hypoxic injury by regulating mitochondrial permeability transition pore opening docx
... TRAP1 expression after hypoxia treatmentas well as the mechanism and effect of TRAP1 on hypoxic damage. The results obtained showed that TRAP1 expression increased after 1 h of hypoxia and continued ... 5¢-CAACAGAGATTGATCAAAT-3¢. A negative control adenovirus vector containingnonspecific siRNA was constructed in the same way (non-specific vector, 5¢-TTCTCCGAACGTGTCACGT-3¢). Allvectors contained ... protein. Hypoxia is a common pathophysiological processin diseases such as shock, stroke and heart failure.Hypoxic damage of the myocardium is relevant notonly to coronary artery diseases, but also...
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Tài liệu Báo cáo khoa học: LAT – an important raft-associated transmembrane adaptor protein pptx
... Tabata H, Jeon MS, Hayashi K, Tanaka Y,Krishna R, De Giorgio L, Liu YC, Fukata M & Alt-man A (2006) Impaired activation and localization of LAT in anergic T cells as a consequence of a ... anotherTRAP, PAG [85]. Regulation of palmitoylation of LAT (and of other palmitoylated membrane proteins) and its functional consequences remain a ratherunknown and potentially very important area. The ... suggest a surprising additional mechanism of LAT participation in the TCR signalling process. The involvement of LAT-containing membrane raftsin the formation and signalling of TCR microclustersand...
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