Báo cáo khoa học: Dynamics of a-synuclein aggregation and inhibition of pore-like oligomer development by b-synuclein doc
Báo cáo khoa học: MAP2 prevents protein aggregation and facilitates reactivation of unfolded enzymes ppt
... results of investigation of chaperone-like activity of MAP2 usingdithiothreitol-induced aggregation of insulin at 25 °C. Here,the inhibition of aggregation is about 70% in the presence of 0.1 ... (w/w) of 0.55,1.1 and 2.2, the inhibitions of insulin aggregation wereobserved to be 60, 70 and 90%, respectively (curves 2, 3 and 4). With pure tubulin near identical experimental conditions and ... wenoticed excellent chaperone-like activity of MTP. MTPprevents chemical and thermal aggregation of other proteins and can enhance the extent of refolding of fully unfoldedsubstrate enzymes. Because...
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Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx
... were separated by SDS/PAGE and stained by Coomassie brilliant blue. The twohemocyanin bands were cut out and dispersed. About100 lg of hemocyanin was used for the immunization of rabbits. For ... submitted to MALDI-TOF analysis and assigned to distinct subunits.Fig. 1. SDS/PAGE and immunoblotting of S. coleoptrata hemocyanin.About 10 lg of total hemolymph protein (HL) and 3 lgofpurifiedhemocyanin ... Gebauer and Markl [14] identified fourdistinct subunit types by means of immunological studies,native and denaturating PAGE. Our MALDI-TOF experi-ments and cDNA sequencing confirm their results. By comparison...
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Báo cáo khoa học: Photosynthetic acclimation: State transitions and adjustment of photosystem stoichiometry – functional relationships between short-term and long-term light quality acclimation in plants ppt
... appear-ance of clouds and the leaf movement by wind. Bioticinfluences depend mainly on the individual position of a plant within an ecosystem, the density of the plantstand or, in case of algae, ... the surroundings of Jena). The range of photosynthetically activeradiation (400–700 nm) and the absorption maxima of PSII and PSI(680 and 700 nm) are marked along the top of the figure. Condi-tion ... expression of photosystem genes and in the accumulation of Chl a and Chl b [29]. Despite the differences in the timescales of action, the LTR-like state transitions are triggered by the redox state of...
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Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt
... preventing the aggregation of luciferase. ClpC1D2 without ATP had no effect onthe heat-induced aggregation of luciferase (Fig. 6C).There was some aggregation of ClpC1 and ClpC1D2inthe presence of ATP ... preventprotein aggregation and translocate them across cellmembranes [3]. Increased expression of heat shockproteins is triggered by a range of stress conditions, and is also induced in both the host and ... Construction and purification of M. tuberculosis ClpC1 and its deletion mutants. (A) SDS ⁄ PAGE of purified full-length ClpC1 and deletion mutants, ClpC1D1 and ClpC1D2. (B) Full-length ClpC1 and deletion...
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Báo cáo khoa học: Site-directed mutagenesis, kinetic and inhibition studies of aspartate ammonia lyase from Bacillus sp. YM55-1 pptx
... obtained from grafit software.Kinetics of reversible inhibition of AspB, T141A and H188A by 3-nitropropionate and D-malateThe reversible inhibition of AspB and the T141A and H188A mutants was ... pKavalues of pK1= 6.8 ± 0.1 and pK2= 11.1 ± 0.5were found. For the H188A mutant, pKavalues of pK1= 7.4 ± 0.3 and pK2= 9.4 ± 0.3 were found.Competitive inhibition of AspB and the T141A and ... mecha-nism of the AspB-catalyzed reaction and pKavalues of potential acid and base catalysts by pH-rate studies.From the ascending limb of the pH-rate profile of AspB, a residue with a pKa of 7.1...
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Báo cáo khoa học: "Dynamic programming for parsing and estimation of stochastic unification-based grammars∗" pot
... =y} and F(y) =ω∈Ω(y){f ∈ ω}. That is, Ω(y) isthe set of parses of a string y and F(y) is the set of features appearing in the parses of y. In the gram-mars of interest here Ω(y) and hence ... also F(y) arefinite.Maxwell and Kaplan’s packed representations of- ten provide a more compact representation of theset of parses of a sentence than would be obtained by merely listing each parse ... value thatmaximises a product of functions or else as the sum of a product of functions, each of which dependson a small subset of the variables X. These are thekinds of quantities for which dynamic...
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Báo cáo khoa học: Mixed lineage kinase LZK and antioxidant protein-1 activate NF-jB synergistically docx
... the amount of IKKb protein in thesamples, aliquots of cell lysates were first subjected to West-ern blotting, followed by estimation of the amount of IKKbexpressed in each sample by quantitative ... Synergistic activation of NF-jB by LZK and AOP-1 (Eur. J. Biochem. 270)77The kinase reaction was carried out in kinase reaction buffercontaining 0.5 lCi of [c-32P] and 5 lgofGST-IjBa(1–54)for ... three types of protein kinases, MAPK,MAPK kinase (MAPKK) and MAPKK kinase (MAPKKK). Upon activation, MAPKKK is first activated by anextracellular stimulus, and then phosphorylates and acti-vates...
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Báo cáo khoa học: Dynamics of a-synuclein aggregation and inhibition of pore-like oligomer development by b-synuclein doc
... Modeling of a-syn oligomer formationFEBS Journal 274 (2007) 1862–1877 ª 2007 The Authors Journal compilation ª 2007 FEBS 1877 Dynamics of a-synuclein aggregation and inhibition of pore-like oligomer ... b- and b-syn, and b- and a-synheterodimeric interactions. Firstly, theoretical docking of various molecular dynamics conformers of a-syn toconformers of b-syn was performed. All of the dockeda-syn–b-syn ... recent study has analyzed by NMR the micelle-bound structure and dynamics of b- and c-syn [41].Thus, better understanding of the steps involved inthe process of a-syn aggregation is important...
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Báo cáo khoa học: Refined solution structure and backbone dynamics of the archaeal MC1 protein ppt
... MHz, and R2relaxation delays of 10, 20, 30, 50, 70, 90, 110 and 150 mswere used for data collection at 800 MHz. The errors inR1 and R2were determined by generating random distri-butions of ... variability isreflected by extensive DNA contacts between 9 bp of DNA and the b-ribbon arms, and variable contactsbetween additional DNA and the body of the protein[19]. In the case of MC1ÆDNA complexes, ... that thearm of MC1 is essential for DNA binding and bend-ing. The interaction mode of MC1 is probably com-pletely different from those of Sac7d and Cren7, whichbind and bend DNA by placing...
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Báo cáo khoa học: Dynamics of flavin semiquinone protolysis in L-a-hydroxyacid-oxidizing flavoenzymes – a study using nanosecond laser flash photolysis doc
... kinetics of proton transfersin an individual system is of prime importance inunderstanding the fine details of the processes involvedat the functional and structural levels. However, the dynamics of ... obtained by proton ejection from a photodis-sociable dye. Time-resolved studies of proton transferfor a number of proteins using this method have beenreviewed by Gutman and Nachliel [2] and Aădelroth and ... by means of a septum, and the solutions were exposed at 23 ° C to the visible light froma DC Xe lamp, ensuring photoreduction of the flavin by the EDTA present in the solutions. In the case of...
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