Atlas of Human Pluripotent Stem Cells doc
Atlas of Human Pluripotent Stem Cells doc
... Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder Layer-Free Conditions 41 3.1 Introduction 41 3.2 Materials for Feeder Layer-Free Culture of hPSCs ... Abstract Human embryonic stem cells (hESCs) are pluripotent cells derived from the inner cell mass (ICM) of the developing embryo. They have tremendous poten-tial for the research of early human ... (VEGF-A165) ZP Zona pellucida 1M. Amit and J. Itskovitz-Eldor, Atlas of Human Pluripotent Stem Cells: Derivation and Culturing, Stem Cell Biology and Regenerative Medicine,DOI 10.1007/978-1-61779-548-0_1,...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... and power failure. Key words: human embryonic, stem cells, low temperature 1. Introduction In vitro culture of human embryonic stem cells (hESC) often involves temporary exposure to reduced ... Published: 2006.07.25 This study investigated the effects of exposing human embryonic stem cells (hESC) to 4oC and 25oC for extended durations of 24h and 48h respectively. Cell survivability after ... temperature tolerance of human embryonic stem cells Boon Chin Heng 1, Kumar Jayaseelan Vinoth 1, Hua Liu 1, Manoor Prakash Hande 2, Tong Cao 1 1. Stem Cell Laboratory, Faculty of Dentistry,...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... murine ES cells and in human hematopoietic progenitor cells. DiscussionThe commitment of pluripotent stem cells to succes-sively less plastic progenitors and, finally, differentiated cells exhibiting ... developmental progres-sion of cell samples and in all cases except that of theRex-1 gene, primer sets span introns. Day 0 cells arecomposed of ES cells and MEF cells grown in ES mediacontaining ... andtemporal expression of specific subsets of genes. Thesequential silencing of genes involved in maintenance of pluripotent and multipotent states and the activa-tion of those involved in differentiation...
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Báo cáo khoa học: MicroRNA-23b mediates urokinase and c-met downmodulation and a decreased migration of human hepatocellular carcinoma cells doc
... none of those considered (i.e. size of the tumor, degree of differ-entiation, and the presence of pseudocapsule invasion) wasrelated to miR-23b expression in HCC.3¢-UTR constructsSegments of ... correct orientation of the insert was verifiedby sequencing.Transient transfection of SKHep1C3 cells withmiR-23b and transient transfection of AB2 cells with anti-miR-23bMolecules of dsRNAs that ... areinvolved in human cancer, through the developmentand progression of several types of malignant tumor.An essential role of uPA and c-met in the migrationand proliferation of human hepatocellular...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... specific features of the plasticity of adult stem cells are largelyunknown. Recently, we demonstrated the hepatic differentiation of human adipose tissue-derived mesenchymal stem cells (AT-MSCs). ... GM, Claas FH, Fibbe WE & KanhaiHH (2004) Isolation of mesenchymal stem cells of fetalor maternal origin from human placenta. Stem Cells 22,1338–1345.6 Bieback K, Kern S, Kluter H & ... evidence of transdifferen-tiation by MET in the process of hepatic differen-tiation of AT-MSCs. No previous report hasdemonstrated evidence of transdifferentiation of com-mitted adult stem cells. ...
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Báo cáo khoa học: Modulation of IMPDH2, survivin, topoisomerase I and vimentin increases sensitivity to methotrexate in HT29 human colon cancer cells docx
... produced upon treatment of cells with MTX, using cDNA arrays that allow thesimultaneous evaluation of expression, at the mRNAlevel, of hundreds of genes. Specifically, the Atlas Human Cancer 1.2K ... One microgram of pcDNA-VIM or 0.5 lM aODN-VIMwere lipofected either in the absence or in the presence of 10)8M of MTX in parental HT29 cells (A) or 10)5M of MTX in HT29-R cells (B).Twenty-four ... expression profile of the 1176 genes included inthe Atlas Human Cancer 1.2K Array (ClontechÒ) wasanalyzed in parental HT29 cells, HT29 cells treatedduring 24 h with 10)7m MTX, or HT29-R cells resist-ant...
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Báo cáo khoa học: Effects of the antioxidant PycnogenolÒ on cellular redox systems in U1285 human lung carcinoma cells docx
... effect of Pycnoge-nolÒon pure TrxR1 enzyme activity (data not shown).Effects on the viability of U1285 cells The toxicity of the compounds used was assessed bymeans of determination of the ... selenium supplementation [31], probably due to thehigher ranking of TrxR1 in terms of the hierarchy of selenoenzymes [32]. Treatment of cells with an organicselenium compound, SeMC, did not cause ... This is demonstratedby the effect of addition of PycnogenolÒafter selenitetreatment, which decreases the activity of TrxR tolevels below those of untreated cells, even though sele-nite alone...
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Báo cáo y học: "ene expression analysis of human red blood cells"
... Understanding of molecular mechanisms governing the enucleating phenomena of human erythrocytes is of major importance in both fundamental and applied studies. Total RNA (n=7) from human RBCs (purity of ... 1). Table 1 The purity of human RBCs fraction tested by independent methods. blood cell count (n=5) RBCs (cells/ µL) white blood cells (cells/ µL) platelets (cells/ µL) cytometer ... Analysis of total human RNA of RBCs (Bioanalyzer 2100 Agilent, USA): A) L- RNA 6000 ladder (Ambion, USA); 1-3 - total RNA of human RBCs from different donors; B) typical electropherogram of total...
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Tài liệu Color Atlas of Pharmacology (Part 5): Pharmacokinetics docx
... DoseAmount excreted per unit of time [amount/t]Notional plasma volume per unit of time freed of drug = clearance [vol/t]Unit of timeTimeCoLüllmann, Color Atlas of Pharmacology © 2000 ThiemeAll ... concentra-tions of about 0.02 %, and the amount of drug eliminated per unit of time re-mains constant at concentrations abovethis level.44 PharmacokineticsL llmann, Color Atlas of Pharmacology ... drug. The ratio of AUCvalues determined after oral or intrave-nous administration of a given dose of aparticular drug corresponds to the pro-portion of drug entering the systemiccirculation...
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Tài liệu ENCYCLOPEDIA OF HUMAN NUTRITION SECOND EDITION docx
... years. They see life interms of ending and the younger version of immortality is not a part of their vision. Also, life events can change their view of the importance of dietary adherence in a positive ... Strategies Methods of Tailoring Modeling Model preparation of quick, four-ingredient dessert parfaitSelf-Monitoring Include two simple forms of self-monitoring that allow for a check-off of items ... tailored to the needs of the participants in the study. For example,often sessions described the nutritional benefits of certain types of fruitsand vegetables. The types of fruits and vegetables...
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