new treatment modalities by disease specific and patient specific induced pluripotent stem cells
báo cáo khoa học: "A nano-view of West Nile virus-induced cellular changes during infection" pps
... America [13] With emerging and re-emerging viruses such as this, new and pertinent data of how these viruses replicate in their host cells is becoming increasingly and urgently needed The nano-biotechnological ... preparation and AFM studies NML conceived of the study, and participated in its design and coordination Both authors read and approved the final manuscript Publish with Bio Med Central and every ... very compatible for virus and cell imaging In the case of West Nile (Sarafend) virus infections, the new information provided by the AFM gave further insight into virus -induced changes during the...
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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: intersubunit interactions during virus assembly doc
... are small, globular and mainly helical NTD contains a-helices 1–7 of CA, and is connected by a flexible linker to CTD, which contains a small 310-helix, an extended strand and a-helices 8–11 of ... at the NTD–NTD interface (by mutations Ala14Cys and Glu45Cys) and the substantial weakening of CA–CA dimerization through the CTD–CTD interface (by mutations Trp184Ala and Met185Ala) The X-ray ... CA during the assembly of both the immature and the mature capsid of HIV-1 and other retroviruses Induced conformational switching in CTD and ⁄ or NTD and ⁄ or alterations in the linker region...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... (A), as determined by Schuster et al [13] and by Smith et al [14], and domain II, as determined, respectively, by Smith et al [14] (B), Schuster et al [13] (C) or predicted by RNA Draw software ... interacts with the 3¢X sequence of the 3¢UTR by binding the stem of the SL-II stem loop and the hinge between SL-I and SL-II [12] More precise analysis by site directed mutagenesis in the context ... Astier-Gin et al plus-strand RNA has been determined [10,11] and the involvement of the three stem loops of the 3¢X has been extensively studied both in vitro, in the replicon system and in vivo [7–9,12]...
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Báo cáo hóa học: " Therapeutic immunisation of rabbits with cottontail rabbit papillomavirus (CRPV) virus-like particles (VLP) induces regression of established papillomas" docx
... the adjacent cells and tissues triggered by immune recognition of a primary target, also called a bystander effect [23] Thus the levels of L1 would indeed be detected by the immune system but would ... prophylactic quadrivalent HPV (types 6, 11, 16 and 18) L1 recombinant VLP vaccine (Gardasil) produced by Merck was approved and registered by the Food and Drug Administration (FDA) on the June 2006 ... and complete protease inhibitor (Roche), and sonicated The sonicated suspension was centrifuged at 100,000 g at 10°C for 24 h Two distinct bands were observed on the CsCl gradient: the top band...
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Báo cáo hóa học: " Molecular and macromolecular alterations of recombinant adenoviral vectors do not resolve changes in hepatic drug metabolism during infection" potx
... and μl of QuantumRNA™ 18S internal standard (Ambion, Austin, TX) at a primer:competimer ratio of 4:6 for CYP 3A2 and 2C11 and 3:7 for PXR and RXR reactions Gene -specific reaction conditions and ... reduced by 63% and 15% in animals given the AdlacZ and PEGAd viruses (p ≤ 0.05) By 14 days, mRNA levels in animals treated with PEGAd and HDAd had recovered to baseline while those treated with WT and ... hepatic CYP2C11 mRNA resulted from treatment with AdlacZ (56%) while the WT, PEGAd and HDAd treatments reduced mRNA by 36, 31, and 21% (Figure 6B, p ≤ 0.01) By day four, animals treated with the...
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Báo cáo hóa học: " Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses" pot
... target cells and expression of GFP in transduced cells is indeed an accurate reflection of the infection steps mediated by the surface glycoproteins of retrovirus-derived VLPs [17,21,22] and, hence, ... viruses, by transient expression in 293T cells of surface (HA, NA, M2) and internal (Gag, GFP marker genome) viral components Expression of the different viral proteins in producer cells and their ... contributions JS, DK and FLC conceived the study JS and FLC coordinated the research efforts and edited the manuscript BB, PJ, and MM contributed to parts of the experimental work MM, HDK, DK and VV provided...
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Báo cáo khoa học: " Serum interferon-gamma and interleukins-6 and -8 during infection with Fasciola gigantica in cattle and buffaloes" ppsx
... mechanism by which flukes are destroyed In F hepatica-resistant rats larvae of F hepatica were coated with antibody and host cells, including eosinophils, neutrophils, macrophages and mast cells, ... identified as the release of high levels of nitric oxide by peritoneal lavage cells [22,23,27] Cattle and buffaloes, by producing IL-6 and IL-8 (in buffaloes) during infection with F gigantica, ... Elizabeth C Molina Fig IFN-γ profile in cattle (a) and buffaloes (b) infected with F gigantica and non-infected cattle and buffaloes were determined by ELISA The assay made use of mouse anti-ovine...
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Báo cáo y học: "Altered expression of inflammatory cytokines in primary osteoarthritis by human T lymphotropic virus type I retrovirus infection: a cross-sectional study" pps
... several inflammatory cytokines in SF between HTLV-I carrier patients and HTLV-I-negative OA patients By investigating OA patients only and excluding those with RA, we were able to demonstrate the ... simultaneously from 22 HTLV-I carrier outpatients at the initial examination and were subjected to appropriate pretreatment as described previously [18] As patient control, SF and serum were also obtained ... obtained from 58 HTLV-I-negative OA patients Comparison of HTLV-I carrier and non-carrier OA patients showed no obvious differences in age, sex, affected side and disease duration (Table 1) At the...
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In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles pptx
... affected by IL-10 shRNA after LPS treatment in RAW264.7 cells by ELISA (Fig 3E) Figure 3F shows reduction of IL-10 by IL-10 shRNA after LPS treatment in RAW 264.7 cells at 36 and 48 hours by ELISA ... contributions MIC and YFH contributed equally to this work MIC and YFH interpreted the data and drafted the manuscript MIC, MW, DC and JTC designed and performed the RNAi and JC virus experiments MZ and GJT ... irrelevant shRNA, respectively After 6, 12 and 24 h of treatments, serum samples were collected and tested for IL-10 and TNF-α levels by real-time PCR and cytokine enzyme-linked immunosorbent assay...
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báo cáo khoa học: "Stability and assembly in vitro of bacteriophage PP7 virus-like particles" pptx
... R250 The gel was scanned with a densitometer and the quantity of protein in individual bands was determined by comparison to a standard curve produced by applying dilutions of a known quantity of ... the 5'- and 3' is shown at ThePP7 bottom, and the sequences2PP7itduplication ends of The sequence at the junction of the 2PP7 duplication is shown at bottom, and the sequences of the 5'- and 3' ... spaced main bands that likely correspond to circular pentamers and hexamers, and two less intense bands representing linear pentamers and hexamers (i.e "nicked" circles) This is in accordance with...
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Thermal Stability of RNA Phage Virus-Like Particles Displaying Foreign Peptides potx
... created by insertion of several specific foreign peptides, as well as a library of random-sequence peptides, to produce the constructs shown in Figures 1, and VLPs were expressed in E coli and purified ... VLPs (A) Coincidence of VLP disassembly, as determined by loss of the VLP band on an agarose gel, and coat protein denaturation, determined by disappearance of soluble protein as a function of ... created a complex random-sequence peptide library by insertion of ten NNS triplets between codons for amino acids 13 and 16 in the MS2 AB loop The denaturation profile of the random 10-mer library...
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Báo cáo y học: "Kinetics and isotype profile of antibody responses in rhesus macaques induced following vaccination with HPV 6, 11, 16 and 18 L1-virus-like particles formulated with or without Merck aluminum adjuvant" ppt
... double-stranded DNA viruses Infection with HPV is the most common viral sexually transmitted diseases worldwide [4] HPV infects cutaneous and mucosal epithelial cells and causes benign and malignant ... be specific and sensitive for the detection of rhesus macaque IgM, IgA, IgE, IgG4 and total IgG Low cross reactivity was seen with the anti-IgG1 and IgG2 antibodies to purified human IgG2 and ... Figure HPV 6, 11, 16 and 18 L1 VLP -specific total IgG antibody titers HPV 6, 11, 16 and 18 L1 VLP -specific total IgG antibody titers Sera from rhesus macaques immunized at week 0, and 24 with µg each...
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Báo cáo y học: " Human herpes virus 8 replication during disseminated tuberculosis in a man with human immunodeficiency virus: a case report" pdf
... pyrazinamide treatments, and his condition subsequently improved The patient was started on highly active antiretroviral therapy (HAART) two months later and once the antituberculosis treatment ... diagnosis was expanded to include MCD This was supported by the clinical presentation and the high HHV8 viral load measured in the patient' s blood plasma Finally, the histology and positive PCR ... supported by the fact that the patient was apparently cured by antituberculosis treatment This evolution, as well as two lymph node biopsies without evidence of MCD made it unlikely that the patient...
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Báo cáo y học: "Lassa virus-like particles displaying all major immunological determinants as a vaccine candidate for Lassa hemorrhagic fever" ppt
... studies LASV patient sera specifically recognize VLP antigens in conformational and individual recombinant viral proteins LASV -specific IgM and IgG titers in convalescent subjects and patient sera ... LASV VLP by sucrose gradient sedimentation and detection of GP1, GP2, NP, and Z proteins in fractions by western blot analysis LASV VLP were precipitated with PEG-6000/NaCl and concentrated by ultracentrifugation ... supported by Department of Health and Human Services/ National Institutes of Health/National Institute of Allergy and Infectious Diseases Challenge and Partnership Grant Numbers AI067188 and AI082119,...
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Báo cáo y học: " Self-assembly of virus-like particles of porcine circovirus type 2 capsid protein expressed from Escherichia coli" pptx
... grid was stained by PTA and visualized by TEM Results Expression, purification, and characterization of Cap protein The whole Cap gene was amplified by PCR of PCV2 genomic DNA and cloned into ... particles and present viral antigens in a more authentic conformation and biological function Therefore, they are easily recognized by the immune system and able to stimulate both B-cell and T-cell ... plasmid was diluted 1:50 in 0.2 L fresh LB medium and incubated at 37°C until cells reached mid-log growth The remainder of the culture was induced by the addition of isopropyl-bthiogalactopyranoside...
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Báo cáo khoa học: " The inhibition of assembly of HIV-1 virus-like particles by 3-O-(3'''',3''''-dimethylsuccinyl) betulinic acid (DSB) is counteracted by Vif and requires its Zinc-binding domain" pdf
... DSB on HIV-1 VLP production by (A) insect cells and (B) mammalian cells Effects of DSB on HIV-1 VLP production by (A) insect cells and (B) mammalian cells (A), Sf9 cells infected with AcMNPV-Pr55Gag ... indicated on top of panels (a) and (b), and the x-axis of panel (c) Cells were harvested at 48 h pi, and whole cell lysates (WCL) and extracellular VLP analyzed by SDS-PAGE and immunoblotting Blots ... indicated on top of panels (a) and (b), and on the x-axis of panel (c) Cells were harvested at 48 h pi, and whole cell lysates (WCL) and extracellular VLP analyzed by SDS-PAGE and immunoblotting, using...
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Báo cáo y học: " Kinetics of hepatitis C virus RNA load during pegylated interferon alpha-2a and ribavirin treatment in naïve genotype 1 patients" potx
... week 2, 4, 8, and 12 of treatment, week point time tend to be better than other time points for predicting SVR, with a PPV of 91% and a NPV of 89% and a very good sensitivity and specificity (despite ... predictive of response to treatment [11] In the present study, viral kinetics during Peg-IFN alpha2a plus ribavirin treatment were determined at week 2, 4, 8, and 12 of treatment in 20 patients after a ... studies, positive and negative predictions of SVR using viral load kinetics have been established and now serve as recommendations on antiviral therapy management both by American and by European international...
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Báo cáo sinh học: "The recombinant adeno-associated virus vector (rAAV2)-mediated apolipoprotein B mRNA-specific hammerhead ribozyme: a self-complementary AAV2 vector improves the gene expression" docx
... determined by RT-PCR, followed by Southern blot analysis As shown in Fig 2A, RB15 RNA was detected by RT-PCR and Southern blot analysis at days and after infection There was no detectable band in ... human hepatoma cell line (HepG2 cells, × 105 cells) was seeded onto each well of a six-well plate until the cells reached 80% confluence (~5 × 105 cells) The cells were infected with × 1010 particles ... quantitative RT-PCR using ABI 7700 Sequence Detection System (Applied Biosystems) The sequence -specific primers and probes used for the mouse apoB mRNA and the endogenous control 18S ribosomal RNA were...
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Báo cáo sinh học: " Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivo dendritic cells and T lymphocytes" ppsx
... control; lanes C and D: RNA from transduced 293T cells with and without DNase treatment prior to reverse transcription; lanes E and F: RNA from mock transduced cells treated as for C and D Primer ... and and Efficiency LA34/VSV-G as determined in CrFK, 293Tvariously Efficiency and duration of transduction by naïve and variously modified LA34/VSV-G as determined in CrFK, 293T and NIH-3T3 cells ... the restriction enzymes BclI and SpeI (New England Biolabs, Celbio, Milan, Italy), filling up the protruding ends with Klenow DNA polymerase (New England Biolabs) and joining of resulting blunt-ends...
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Báo cáo khoa học: The association of viral proteins with host cell dynein components during virus infection pdf
... dynein light chains DYNLL1, DYNLT1 and DYNLT3, and a strong interaction could be detected between both UL35(VP26) and UL46(VP11 ⁄ 12) and the dynein light chain DYNLT1 and its homolog DYNLT3 [61] In ... the central nervous system and causes lethal encephalitis in animals and humans After receptor binding, RV enters its host cells through the endosomal pathway via a low-pH -induced membrane fusion ... simultaneously by two independent groups used the rabies and Mokola virus phosphoproteins as baits in yeast two-hybrid screens and retrieved DYNLL1 as an interacting partner using PC12 cells and human...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25