... SNO2,SNO3, SNZ2 and SNZ3 complement the defect. The relationship of all of these genes remains to be clarified.Expression and purification of Sno1p and Snz1pIn light of the similarity in the amino ... hydrolysis mediated by the complex of Sno1p with a His-tag and Snz1p. The reaction wascarried out in 1 mL of 50 mMTris/HCl, pH 8.0, in the presence of 1–10 mMglutamine and 50 lg of the complex, ... primers. Both of the genes were constructed either with or without a His-tag at the 3¢ terminus of the coding region. Thus, the stop codon of SNO1 and SNZ1 was replaced with bases coding for the sequence...