Báo cáo khoa học: "Formal aspects and parsing issues of dependency theory Vincenzo Lombardo and Leonardo Lesmo" potx

Báo cáo khoa học: Limited mutagenesis increases the stability of human carboxypeptidase U (TAFIa) and demonstrates the importance of CPU stability over proCPU concentration in down-regulating ﬁbrinolysis doc

... with (i) a mixture of A115 and F23, (ii) a mixture of A115 and F23, (iii) Y353 and (iv) A115.Table 5. Kinetic and stability parameters for puriﬁed WT and mutant CPUs. The T1 ⁄ 2 of activity at 37 ... instability of CPU is likely to be of majorimportance for the in vivo down-regulation of its activity, but the under-lying structural mechanisms of this fast and spontaneous loss of activity of CPU ... demon-strated similar Kmvalues of the native and WT, and theYQ mutant CPU with a synthetic and physiologicalsubstrate of CPU. There seem to be no major changesin the positioning of the propeptide, as...

Báo cáo khoa học: Microcin J25 induces the opening of the mitochondrial transition pore and cytochrome c release through superoxide generation doc

... effect of MccJ25,which strengthens the hypothesis that MccJ25 gener-ates ROS, regardless of the presence of calcium.DiscussionUnderstanding the mechanisms of action of some types of proteins and ... uptake of calcium through the uniport of calcium. On the other hand, KCN and antimycin A,both inhibitors of electron transport, also inhibited theswelling induced by MccJ25. The inhibition of swellinginduced ... structural and func-tional state of mitochondria seems to be important inthe development of apoptosis-regulating technologies,particularly for anticancer therapy, and for the design of new classes of...

Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx

... dependence of the activity of the third kinase, we varied the Vmax of the secondkinase reaction and recalculated the concentration of the active form of the third kinase as a function of time. ... the linear range of the method. The standard error of the mean was 4.6% of the measured value.AcknowledgementsWe thank Boris N. Kholodenko for intensive discus-sions, and him and Mark Peletier ... transduction on transcription of downstream genes might not just be a function of the amplitude of X3P. Other aspects of signalingdynamics may be important as well, such as frequency of recurrent pulses...

Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

... in thelevels of HSF1 and MTA1 (a component of the NuRDcomplex containing HDAC1 and HDAC2) proteins and to binding of HSF1 to MTA1 [78]. The complexis recruited to the promoters of estrogen-responsivegenes ... consisting of multiple invertedrepeats of the pentanucleotide sequence nGAAn. A single HSF of yeastcan bind to heat shock elements that differ in the conﬁguration of thenGAAn units and can regulate ... senses heat and oxidativestress, and becomes able to trimerize and bind to theHSE [8,9]. Drosophila HSF and mammalian HSF1have a third HR, HR-C, adjacent to the carboxy-ter-minus of the protein...

Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

... because of its higher fold of upregula-tion in gastric adenocarcinoma tissues. Although theextent of upregulation of miR-23a in the four pairs of gastric tissues showed great variance because of the ... the regulatory effects of miR-23a on theexpression of IL6R by RT-PCR and western blot, and found an inverse correlation between miR-23a and IL6R expression at both the mRNA and protein levels.To ... thatwere predicted by two of the algorithm programs(pictar and targetscan release 5.1) and were alsodownregulated in our cDNA microarray were selectedas candidate targets of miR-23a. Among these...

Tài liệu Báo cáo khoa học: The miRNA-192 ⁄194 cluster regulates the Period gene family and the circadian clock doc

... Examination of BMAL1mRNA oscillation over a time course of 64 h revealedABCD***Fig. 2. Validation of the effect of miR-192 ⁄ 4 on the Per 3¢UTRs.(A–C) Veriﬁcation of the effect of miR-192 ... repre-sented as the standardized mean ± standard error of the mean(SEM). HCT116 cells express low levels of miR-192 ⁄ 194, whereasLOVO and HT29 cells express high levels of this miRNA cluster.(B) ... exact role of miR-192 ⁄ 194 in thesetissues. Together, the identiﬁcation of inhibitorymiRNAs for the Per genes adds more complexity tothe mode of regulation of core components of thecircadian...

Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane afﬁnity and hyper-anticoagulant activity in mouse plasma pptx

... All other reagents and buffer components werecommercially available and of highest the purity.Mutagenesis, construction and stable expression of recombinant mouse protein C and variantsMutagenesis ... Kirchhofer D(1996) The crystal structure of the complex of bloodcoagulation factor VIIa with soluble tissue factor.Nature 380, 41–46.37 Small DM (1967) Phase equilibria and structure of dry and ... protein–membraneinteraction and identify new reagents with varyingdegrees of anticoagulant potency that may be of usefor testing in murine models of sepsis and thrombo-embolic disorders.ResultsExpression and characterization...

Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression proﬁling ppt

... established a lack of fluctuation in the proteinlevels of AaEcR and AaUSP isoforms during vitello-genesis [13], suggest a lack of significant transcriptional and translational regulation of the two componentsthat ... isoforms, eachwith a distinctive N-terminal A ⁄ B domain [36].AaE75B cannot bind DNA due to the lack of one of its zinc fingers. All three isoforms present a similarpattern of expression and ... ormore ligands, although the nature of that remainsundetermined [72]. Several lines of evidence have sug-gested a role for vertebrate ERRa and ERRc in thecontrol of metabolic genes. Both isoforms...

Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

... Ophthalmology and Visual Sciences, College of Medicine, University of Illinois, Chicago, IL, USA2 Department of Pathology, College of Medicine, University of Illinois, Chicago, IL, USA3 Department of ... mediates entry of HSV-1 into human T lymphocytes and trabecular mesh-work cells, and is expressed in many fetal and adulthuman tissues, including the lung, liver, kidney, and lymphoid tissues [7,8,16]. ... Valyi-Nagy T (2006) Expression of entry receptor nectin-1 of herpes simplex virus 1 and ⁄ or herpes simplex virus 2 innormal and neoplastic human nervous system tissues.Acta Virol 50, 59–66.38...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... joints of the hands and feet. Theinflammatory process is characterized by infiltration of inflammatory cellsinto the joints, leading to proliferation of synoviocytes and destruction of cartilage and ... against the development of RA [61].The gene copy number variations of CCL3-like 1(CCL3L1), a nonallelic isoform of CCL3 encoded bydifferent genes and a potent ligand for CCR1 and CCR5, influenced ... Increasedexpression of MIP-3a ⁄ CCL20 and CCR6 has also beenconfirmed in tissue biopsies from RA subchondralbone [27].Our group recently found that the mRNA exp-ression of MCP-4 ⁄ CCL13 (a ligand of CCR2 and CCR3),...

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