Báo cáo khoa học: Dermaseptin DA4, although closely related to dermaseptin B2, presents chemotactic and Gram-negative selective bactericidal activities doc

... ª 2009 FEBS Dermaseptin DA4, although closely related to dermaseptin B2, presents chemotactic and Gram-negative selective bactericidal activities Constance Auvynet1,2,*, Pierre Joanne2,, ... of the DRS -DA4, DRS -B2, DDK, and DRS-L1. Hexagonal and round backgrounds refer to basic and acid amino acids respectively, pentagonal backgrounds to hydrophilic residues, and squares to hydrophobic ... 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine; DMPG,1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol; DRS -B2, dermaseptin B2; DRS-DA3, dermaseptin DA3; DRS -DA4, dermaseptin DA4;DRS-L1, dermaseptin...

Báo cáo khoa học: Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice doc

... 5¢-CTCGAGACAATACAATTGTCCAGG-3¢, and reverse primer 5¢-ACAAAGCTTATTTGTAAGGTTTGTTACA-3¢, and the PCR product was cloned into pBSK+vector using the SmaI restriction site, and then intopRSETB vector using XhoI and HindIII ... and 3), CD69NG70 (lanes 4 and 5), CD69NV82(lanes 6 and 7) CD69NS84 (lanes 8 and 9),rat CD69 (lanes 10 and 11) and mouseCD69 (lanes 12 and 13) was performedunder nonreducing (even lanes) and reducing ... dithiothreitol, and also in 10 mm Pipes buffer (pH 6.8) with 50 mm NaCl,1mm NaN3 and 5 mm dithiothreitol, and in 10 mm Trisbuffer (pH 7.8) with 50 mm NaCl, 1 mm NaN3 and 5 mmdithiothreitol. From...

Tài liệu Báo cáo khoa học: parDtoxin–antitoxin system of plasmid R1 – basic contributions, biotechnological applications and relationships with closely-related toxin–antitoxin systems ppt

... ofthe toxin.The RelE clueUnderstanding the effect of the toxin on ColE1 and kreplication and the protection of Kid toxicity by DnaBE. Diago-Navarro et al. parD ⁄ kid-kis Toxin-Antitoxin systemFEBS ... prolifera-tion and viability. In both systems, the toxin was able to kill, whereas the antitoxin neutralized its action. To further analyse the effect of the toxin and theantitoxin in HeLa cells, ... Specialreference is given to the ccd toxin–antitoxin system of plasmid F becauseits toxin shares structural homology with the toxin of the parD system.Inter-relations with related toxin–antitoxin systems...

Báo cáo khoa học: "Veterinary decision making in relation to metritis - a qualitative approach to understand the background for variation and bias in veterinary medical records"

... decision making connected to metritis treat-ment and potential links to data quality. This understand-ing provides insight into potential errors (bias and random error) related to data based on clinical ... drying off and at calving(5-21 days post partum). The mandatory screenings focuson general condition, metritis/vaginitis, mastitis and body condition. Optional screenings focus on ketosis and limb ... generating score data and decision mak-ing in relation to treatment of metritis and their motiva-tion to produce data. This information was condensedinto a 'model of understanding' that...

Báo cáo khoa học: "Medical emergency teams: deciphering clues to crises in hospitals"

... find more reliably patients who are in crisis, and then respond to them swiftly and effectively to prevent unnecessary deaths.In 1994, Franklin and Mathew [1] recognized that cardiacarrests ... recognized and treated, oftendeath can be prevented. Medical emergency teams (MET) are amechanism to fill this need. The epidemiology of patientdeteriorations is not well understood. Jones and colleaguesprovide ... forHealthcare Improvement and the Society for Critical CareMedicine have been promoting rapid response teams and METs. In North America and in Europe, there now appears to be a rapid increase in...

Tài liệu Báo cáo khoa học: Degradation of tropoelastin by matrix metalloproteinases – cleavage site speciﬁcities and release of matrikines pptx

... taxonomicallyrestricted to ‘Homo sapiens’, the enzyme was set to ‘none’, and the mass error tolerances for precursor and fragmentions were typically set to 0.15 u for qTOF MS ⁄ MS and 50 p.p.m. and 1 u, respectively, ... signals with signal -to- noise ratio higher than 10 were automatically subjected to MALDI-LIFT-TOF ⁄ TOF MS ⁄ MS experiments by applying2000 laser shots.NanoHPLC–nanoESI–qTOF MS analysisOn-line ... has a more polar and larger activesite than MMP-7 and MMP-9 and hence tolerates suchresidues better. The low number or lack of cleavagesby all three MMPs N-terminal to large and polaramino...

Tài liệu Báo cáo khoa học: Enzyme kinetics informatics: from instrument to browser pdf

... experimental instrument,process and normalize it to agreed standards and finally transfer these data to publicly available data-bases to make them accessible. To facilitate the dissemination ... developed to advise on the mini-mum requirements to follow in the storage and dis-semination of experimental data in fields such astranscriptomics and proteomics, which will ultimatelyallow data to ... intention is that any data collected and submitted to the repositories will be complete, consis-tent and adhere to defined standards, such as theSTRENDA recommendations. Although much of this work has...

Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

... core histones is associatedwith heat shock. This is achieved by HSF1 and the his-tone deacetylases HDAC1 and HDAC2 [77]. In heat-shocked cells, HSF1 binds to HDAC1 and HDAC2, and their histone-deacetylase ... (SWI ⁄ SNF, ISW1 and RSC) and histone chaperones (Asf1, Spt6 and Spt16), are involved in histone acetylation and eviction[52–55]. Stress-inducible or constitutive binding ofHSF to a promoter is ... SNF complexes are recruited to both the pro-moter and coding regions, and histones are acetylated and displaced [56–58]. However, the presence of SAGA and SWI ⁄ SNF, and a high density of Pol...

Tài liệu Báo cáo khoa học: The miRNA-192 ⁄194 cluster regulates the Period gene family and the circadian clock doc

... negative regulators ofCLOCK ⁄ BMAL1, the family of Period genes (Per1,Per2 and Per3), the Cryptochromes (Cry1 and Cry2) and Rev-Erb a [3,5,6]. Rev-Erba binds the BMAL1promoter directly to inhibit ... phosphorylation and degradation of Perproteins have been suggested to control timing of themammalian clock [8]. Moreover, BMAL1 and Cryproteins are subject to phosphorylation, SUMOylation and proteasomal ... of post-transcriptional regula-tors, called microRNAs (miRNAs), has been shown to possess regulatory functions towards the circadianclock. miRNAs are single-stranded, nonprotein-codingRNA molecules,...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... Puf4 and Puf5 were shown to bind,respectively, to 56%, 26% and 49% of all known and putative 3¢ UTR sequences possessing the binding con-sensus identiﬁed [23]. The same strategy was used to identify ... (reverse) to APUM-2 and 5¢-CGATGCAGAAATTCAGTAGCAACATGGTGGAACGATGTCTCA-3¢ (forward) and 5¢-GCATGAGACATCGTTCCACCATGTTGCTACTGAATTTCTGCA-3¢ (reverse) to APUM-7.Qualitative and quantitative b-galactosidadeactivity ... 50 to 150 bp were puriﬁed from the gel and ligated to the pRH5¢ vector (Invitrogen) in a single step reaction, usingPmeI and T4 DNA ligase at 25 °C overnight. The ligationmixtures were used to...

Xem thêm