Báo cáo khoa học: Evolution of the teleostean zona pellucida gene inferred from the egg envelope protein genes of the Japanese eel, Anguilla japonica potx

... 4674–4684 ª 2010 The Authors Journal compilation ª 2010 FEBS Evolution of the teleostean zona pellucida gene inferred from the egg envelope protein genes of the Japanese eel, Anguilla japonica Kaori ... wediscuss the evolution of ﬁsh egg envelope genes usingphylogenetic analysis.ResultsPuriﬁcation of envelope proteins from Japanese eel The unfertilized egg envelope proteins of Japanese eelwere ... trees of the nucleotide sequences of the zp genes of Teleostei. (A) Phylogenetic tree of teleostean zp genes. The subfamilies of zp genes are labelled (ZPB, ZPC and ZPX). (B) A tree of teleostean...

Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

... A binding isothermwas ﬁtted to the data, expressed in terms of the heat changeper mole of ligand against the ligand to macromoleculeratio. From the binding isotherm values for the reactionstoichiometry, ... by subtraction of data from a series of injections of copper chelate solutioninto a buffer blank correlating to the heat of dilution of the copper complex. After subtraction of the blank data ... The Authors Journal compilation ª 2006 FEBS 4251 from the 3D structures [12,14]. Therefore, the differ-ences in the far UV CD must be due to the contribution of tyrosines to this region of the...

Báo cáo khoa học: An Escherichia coli twin-arginine signal peptide switches between helical and unstructured conformations depending on the hydrophobicity of the environment potx

... known of the overall structure of these peptides. In this report, we haveanalyzed the secondary structure of the SufI signal peptide,together with those of two nonfunctional variants in which the ... with protein protein interactions involving the two extensivearginyl sidechains.On the basis of these data we suggest the following modelfor the structures of Tat signal peptides during the ... 220 nm. The spectra in the other solvent systems are typical of a-helices, though of Fig. 1. Structure of the SufI signal peptide.Shown are the amino acid sequences of the E. coli SufI twin-arginine...

Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

... the program exprot [23]. Both methodsfailed to confirm the cleavage site of the main adhesineP1 of M. pneumoniae. This is the only other M. pneu-moniae protein, of which the N-terminus of the ... degradation of a protein, followedby the analysis of the cleavage products. It is, how-ever, difficult to prove which of the identified peptiderepresents the amino terminus of the protein. To ... responsible for the processing of the proposed130-kDa protein has not yet been identified. P40derives from the N-terminal and P90 from the C-ter-minal part of the predicted 130-kDa precursor protein. It...

Báo cáo khoa học: Neuroserpin Portland (Ser52Arg) is trapped as an inactive intermediate that rapidly forms polymers Implications for the epilepsy seen in the dementia FENIB ppt

... 0.7 mgÆmL)1. The second derivative of the resulting data was used to calculate the inﬂection point of the transition and hence the Tm[14].Assessment of the polymerization of wild-typeand ... because the signal at 216 nm did not changeduring the course of the experiment, i.e. after incubation of Ser52Arg neuroserpin at either 37 °Cor45°C for 24 h.Assessment of the conformation of Ser52Argneuroserpin The ... among neurodegenerative dis-orders in that there is a striking correlation between the number of inclusions within the cerebral cortex and aninverse relationship with the age of onset of disease...

Báo cáo khoa học: Kinetic and binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacteriumsp. strain HE5 ppt

... under these conditions. However, from the slope of the initial linear range of the kinetic plot, the constants Vmax⁄ Km of 1.1 electronsÆmin)1Ænm)1forNHis-Fdmorand Vmax⁄ Km of 0.9 ... effect on the spin-state of NHis-P450morin the presence or absence of any of the testedN-heterocycles.In order to obtain more information about the bind-ing properties of the active site of P450morand ... of the inactive P420 species. The binding of substrates to cytochromes P450 usu-ally induces transition of the heme from the low-spinstate to the high-spin state, which results in a shift of the...

Báo cáo khoa học: "PARALLEL MULTIPLE CONTEXT-FREE GRAMMARS, FINITE-STATE TRANSLATION SYSTEMS, AND POLYNOMIAL-TIME RECOGNIZABLE SUBCLASSES OF LEXICAL-FUNCTIONAL GRAMMARS" docx

... define the syntax of natural lan- guages. On the other hand, there has been much effort to propose well-defined computa- tional models of transformational grammars. One of these is the one ... node v of a derivation tree is a finite set F of pairs of at- tribute names and their values. F is called the f- structure of v. An lfg G consists of a cfg Go called the underlying cfg of ... dc-lfg G, for each pair (tl, t2) of derivation trees in G, if the f-structure and nonterminal of the root of tl are the same as those of t2, then t] and t2 derive the same terminal string. Let...

Báo cáo khoa học: Selecting cells with different Alzheimer’s disease c-secretase activity using FACS Differential effect of presenilin exon 9 deletion on c- and e-cleavage doc

... above. The density of the Ab-bands was quantitated and Ab-secretion wascalculated relative to the protein concentration of the lysatesprepared from the cells in each well, as determined by BCA protein ... then traﬃc to the nucleus to initiate the transcription of the greenﬂuorescence protein (GFP) gene by binding to the 5Gal-E1b domain of the GFP reporter construct. (B) Transfection of COS-7 cells ... expression of the full length protein with glycosylation of the Gal4binding domain (Fig. 2A; lanes 2–4). Immunoprecipitationwith WO2 depleted the anti-Gal4 reactive protein species from the lysate,...

Báo cáo khoa học: Neuropeptide Y, B-type natriuretic peptide, substance P and peptide YY are novel substrates of fibroblast activation protein-a pdf

... P1.None of the gastrointestinal hormone peptides testedcontain a proline at P1, which may be a cause of the poor FAP cleavage of these peptides (all had a half-life of greater than 15 h). These ... this, in the case of GRF, the rate of DPP4 hydrolysis of longerpeptides (44 amino acids) is higher than that of shorterpeptides (three and 11 amino acids) [44]. Moreover, the longer version of PACAP ... aminopeptidases to further truncate the N-termini of peptides [51], which would widen the range of potential FAP roles in vivo. The extracellular location of FAP and DPP4 meansthat these proteases...

Báo cáo khoa học: Hepatocyte growth factor activator (HGFA): a serine protease that links tissue injury to activation of hepatocyte growth factor pdf

... identiﬁcation of the enzyme would be crucial to understanding the control of HGF action in vivo. The activity of this enzyme,however, was not high enough to allow puriﬁcation of the protein for ... rational that the blood coagu-lation system triggers activation of a growth factorthat promotes angiogenesis. Therefore, the prototypicfunction of HGF may be to maintain the integrity of blood ... activ-ity, because two of the conserved catalytic triad resi-dues of the serine protease domain are substituted. The domains of HGF are very similar to those of pro-teases in the blood coagulation...

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