... production determined by NMR. The maindifference between the conformation clusters lies in theposition of the aromatic ring of salicyl alcohol in thebinding pocket. In clusters 1 and 3, the ring ... pDONR207(Invitrogen, Karlsruhe, Germany) by introducing therequired attB1 and attB2 recombination sites in a two-stepPCR process, and recombined into expression vectorpDESTÔ 17 (Invitrogen) containing ... lgÆmL)1carbenicillin, and expression was induced by 0.2% arabinose for 5 h at 24 °C. The cells were pelleted by centrifugation at 12 000 g for 10 min, and resuspended in 4 mL of extraction buffer consisting of...