. volume of phenol/chloroform/IAA, and once with an equal volume of chloroform/IAA.4. Precipitate the cDNA fragments by adding 2 µL of glycogen (1 µg/µL), 50 µL of 10 M NH40Ac and 650 µL of 100%. Extract twice with 300 µL of phenol/chloroform/IAA, and once with 300 µL of chloroform/IAA. Precipitate by adding 33 µL of 3 M NaOAc, pH 5.3 and 800 µL of 100% ethanol. Mix and incubate at –20°C. degeneracy, and homology of the probe) deter-mines the temperature of hybridization. In general, we want to increase thechance of the formation of the desired hybrid and to minimize the formation of nonspecific...