Prepare of mRNA

Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx

... completely shut off, the expression of genes that are under control of the Pnr ⁄ Tnr cassette.Decoupling of transcription and translation of gfp mRNA To further evaluate the properties of the Pnr ... comparable rate of increase of GFP fluorescence (2 h doubling time) afterABFig. 4. Influence of nitrogen source on mRNA expression and theformation of GFP protein. (A) Fluorescence images of C. fusiformisClone ... todetermine the 5¢ end of the CfNR cDNA, allowingunequivocal identification of the gene’s start codon.On the basis of these data, the CfNR gene is made up of 2619 bp of intron-less sequence...

Influence of Polyferric Sulfate Coagulant on the amoA mRNA Expression of Ammonia Oxidizer in Activated Sludge

... of cDNA of amoA mRNA. The profiles of T-RFLP in Run 2 are shown in Fig. 4. Table 1 shows the species of ammonia oxidizer of the major T-RFs (219, 283, 354 and 491 bp). Although almost all of ... addition of polyferric sulfate does not affect the number of ammonia oxidizer, instead, the amount of amoA mRNA is affected in a short time. Because amoA mRNA is one of the key templates of ammonia ... between mRNA detection and in situ activity of ammonia oxidation. Community analysis of ammonia oxidizer based on amoA mRNA The difference of the effect of polyferric sulfate on each species of...

Tài liệu Control the Creation and Behavior of Classes Now that you have code that can prepare all of the pdf

... developer of the problem. Of course, a developer could ignore your exception and attempt to access the properties of the object, but you did give those other developers ample warning. Also, none of ... instance of the class could exist in your application. In short, in VB 6, you didn't have control over the creation of objects. Technique Visual Basic .NET does give you this kind of control ... 9.4 Control the Creation and Behavior of Classes Now that you have code that can prepare all of the objects you need to access and update data in the database,...

Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

... regulatesthe amount of IL-1b mRNA. Involvement of PIN domain of MCPIP in thestability of IL-1 b mRNA In order to ﬁnd out whether the PIN domain inMCPIP is responsible for IL-1b mRNA regulation, ... exogenous) startsdegradation of IL-1b mRNA and also of its own tran-script. For this reason, the level of mRNA for the wild-type form of MCPIP and the level of mRNA for IL-1b(Fig. 5A, lane 3) were ... 5B).Importance of AREs in regulation of IL-1b mRNA by MCPIPIn order to determine whether AREs are important in mRNA degradation triggered by MCPIP, we usedABFig. 5. Involvement of MCPIP in IL-1b mRNA...

Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

... CTGF/CCN2 mRNA levels were normalized to those of GAPDH and thegraphical representation of the results of phosphorimage scans of the mRNA hybridization signals is shown as well. To compare mRNA expression ... relative amounts of normalized mRNA were plotted as a function of time and the slope of this curve was used to calculate the interval period withinwhich half of the original amount of mRN A had ... biphasically like the mRNA, probablybecause of differences between the half-lives of CTGF/CCN2 mRNA and protein (Fig. 1B); protein turnoverbeing slower that that of the mRNA [21]. Meanwhile,...

Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt

... granular layer of human cerebellum. Table 3 compilesour findings in adult brain tissue of all three species.DiscussionThe distribution of ADHs in the brain is of particularinterest because of their ... differentregions of cortex cerebri. The cellular distribution of ADH3 protein coincides with our findings of mRNA: cortical neurons in deeper layers, hippocampal neuronsand Purkinje cells of cerebellum. ... Burnham Institute, La Jolla, CA, USAThe localization of different classes of alcohol dehydro-genases (ADH) in the brain is of great interest because of their role in both ethanol and retinoic acid...

Tài liệu Báo cáo Y học: Inhibition of nuclear pre-mRNA splicing by antibiotics in vitro pot

... thepre -mRNA, s uggesting that t he inhibition is the r esult of disturbance of t he correct foldin g o f the pre -mRNA into thesplicing-compatible tertiary structure by the charged groups of these ... intron r emoval from pre -mRNA by atwo-step transesteri®cation reaction occurs.The ®rst step of nuclear p re -mRNA splicing is g overnedby a nucleophilic attack of the 2¢ OH of the branchadenosine ... Formation of the spliceosomal complexes A and B was slightly reducedand formation of complex C was completely inhibited byerythromycin, resulting in speci®c inhibition of the secondstep of pre-mRNA...

Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx

... School of Medicine, Dentistry and PharmaceuticalSciences, Japan3 Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, Miyagi, Japan4 Department of ... sequences of exon–intron boundaries of WISP1vx strictly conserve thegeneral rule of mRNA splicing sites in higher eukaryo-tes (Fig. 2B). These ﬁndings indicate that WISP1vx isnot an artifact of ... constitutive expression of the full-length and thedifferentiation stage-dependent expression of WISP1vin vitro.Effect of overexpression of CCN4/WISP1 and itsvariants on the phenotypes of humanchondrocytic...

Báo cáo khoa học: A simple in vivo assay for measuring the efﬁciency of gene length-dependent processes in yeast mRNA biogenesis doc

... in the presence of sublethal amounts of MPA. (B) mRNA levels of the transcription units and strains ana-lyzed in (A) cultured in the absence of MPA. The relative values of the mRNA levels with ... steps of transcription inﬂuence equally theexpression of the reporters, independently of the length of their 3¢-UTR.ResultsAn in vivo assay to measure gene length-dependent efﬁciency of mRNA ... on the GLAM ratios of any majorimpairment of transcription, regardless of the step of mRNA biogenesis where it occurs. To rule out thispossibility, we assayed a wide variety of mutants affec-ted...

Báo cáo khoa học: The 3¢-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR ppt

... thatthe high expression of members of the ELAV/Hu family isresponsible for the MARCKS mRNA stability in PCC7-Mz1 cells.Role of ELAV/Hu in controlling stability of MARCKS mRNA We have demonstrated ... nucleotides[(CUUU)11U8]ofthe3¢-UTR of the MARCKS mRNA confers RNA instability when merged with a reporter gene;(b) this CU-rich sequence of 52 nucleotides of theMARCKS 3¢-UTR is speciﬁcally bound by members of the ... stabilization of theMARCKS mRNA even when PKC was activated.The identiﬁed CU-rich sequence of the 3¢-UTR of theMARCKS mRNA belongs to the AU-rich elements (ARE)previously found in the 3¢-UTRs of some...

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