ffpe tissue samples for the discovery of mirna biomarkers
Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
Ngày tải lên :
03/11/2012, 11:17
... active PKC may exist in the nucleus of melanoma cells, and the presence of active PKC isoforms in the nuclei of many cell types has been confirmed (10-12) Staining of the G361 cells with antibodies ... signal (CLDN1-NLS), there is increased expression of claudin-1 in the nucleus, but there are still large amounts of claudin-1 in the cytoplasm as well B) All of the claudin-1 in the nucleus can be ... CA), was used at a concentration of 10μM for the indicated times The PKA activator, forskolin (Sigma) was used at a concentration of μM for the indicated times Immunofluorescenct confocal microscopy...
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Tài liệu Neurodegenerative Biomarkers in Healthy Elderly docx
Ngày tải lên :
13/02/2014, 18:20
... studies of biological markers for AD The first of these circumstances is the need to identify the requirements of the control selection in the specific study The second is to gather the knowledge ... increasing the knowledge in the second of these circumstances In the introduction, I have therefore tried to show that there is both a need and a possibility of accomplishing this In the case of the ... localisation of the changes Some of the most common criteria are the Khachaturian consensus criteria,41 the Braak neurofibrillary staging system,42 the NIA-Reagan criteria48 and the CERAD49 Neither the...
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Tài liệu Biomarkers in Cancer: An Introductory Guide for Advocates pdf
Ngày tải lên :
14/02/2014, 21:20
... receives the information is called messenger RNA (mRNA) because it carries the information out of the nucleus of the cell and into the cell’s cytoplasm for the second step of the process The transcription ... the absence of therapy Predictive To help identify which patients will respond to which drugs What is the purpose of biomarkers? The second component of the definition refers to the uses of biomarkers ... arise from the project In 2006, the Project announced the completion of the DNA sequence for the last of the human chromosomes This landmark project has provided a wealth of information for researchers...
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Tài liệu Báo cáo khoa học: A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics ppt
Ngày tải lên :
16/02/2014, 08:20
... have previously demonstrated the application of this method to the determination of the glycan structure of a form of AFP [10] However, identification of the details of a glycan structural change ... methylesterification of sialic acid moieties All spectra were obtained in the positive ion mode using MALDI– quadrupole ion trap (QIT)-TOF MS A strategy for discovery of cancer glyco -biomarkers On the basis of the ... kinds of lectins used to capture glycopeptides In the next phase, we will provide additional data for other tissues of the mouse, such as those of the brain, kidney, lung, and testis, and extend the...
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Biomarkers in marine organisms a practical approach
Ngày tải lên :
16/03/2014, 18:10
... 424 nm The size of the lower wavelength peak may depend upon both the previous exposure history of the organism and the reproductive cycle and it is noteworthy that the quantification of the CYP ... the expression of multiple CYP forms Hybridisation of Southern blots of genomie DNA gave further evidence of CYP3A- and C Y P l l A -like genes in M edulis digestive gland REGULATION OF CYP FORMS ... Mytilus species either as the dithionite difference spectra of the carbon monoxide-liganded samples (Stegeman 1985) or the carbon monoxide difference spectra of the dithionite-reduced samples (Ade...
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Báo cáo Y học: Loss-of-function variants of the human melanocortin-1 receptor gene in melanoma cells define structural determinants of receptor function doc
Ngày tải lên :
17/03/2014, 10:20
... to the responsive HBL cells The lack of functional coupling of these cell lines, particularly of DOR cells homozygous for the wild-type MC1R, is therefore perplexing and will be the subject of ... suggests that the Leu93Arg mutation has no effect on the processing of the receptor molecule, at least as studied with the tagged forms of the protein Taken together, these results show that the efficiency ... shift of the displacement curve and by the lower amount of radioactivity bound in the absence of competitor (in spite of the lack of statistically significant differences in the number of binding...
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báo cáo hóa học:" Preclinical evaluation of dasatinib, a potent Src kinase inhibitor, in melanoma cell lines" pptx
Ngày tải lên :
18/06/2014, 15:20
... resuspend the cells and 200 μL of ice cold 70% ethanol was added to the cells The plates were then stored at -20°C for hours After fixing the cells were stained according to the protocol for the TUNEL ... removing the media and replacing it with serum free medium (SFM) for a further 24 hours SFM was removed and the cells incubated for a further hours in media containing serum before the drug was ... added The plates were then stored at 20°C for hours After fixing the cells were stained according to the protocol for the Guava Cell Cycle assay (Guava Technologies) Cells were analysed on the...
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báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
Ngày tải lên :
18/06/2014, 15:20
... guide for others in the field to validate these and other similar assays for assessment of patient T cell response These methods can be applied not only to cancer vaccines but to other therapeutic ... validation of the three methods using TIL cells alone or spiked into normal PBMC samples The performances of the assays were further confirmed using PBMC from immunized patients Assay performance ... insufficient number of cells Accuracy The accuracy of an analytical method describes the closeness of mean test results (detected) obtained by the method to the true value (expected) of the analyte Accuracy...
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báo cáo hóa học:" Clinical values of multiple Epstein-Barr virus (EBV) serological biomarkers detected by xMAP technology" potx
Ngày tải lên :
18/06/2014, 15:20
... perform a serial analysis of these EBV parameters during NPC treatment and follow-up In the most patients, the kinetics of the eight anti-EBV antibodies was consistent In 15 of the 35 patients the ... healthy populations For example, sixties had higher levels than any of the other groups for IgA-p18 and IgG-gp78 and twenties had lower levels than any of the other groups for IgA-EA and IgAgp78, ... file 1) Therefore, ROC analysis was consequently utilized to check the diagnostic values of these serological biomarkers for NPC As shown in Table 2, the areas under ROC curve (AUCs) of IgA-EBNA1,...
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Báo cáo sinh học: " Unusual presentation of hepatitis B serological markers in an Amerindian community of Venezuela with a majority of occult cases" doc
Ngày tải lên :
18/06/2014, 18:20
... tested for the presence of HBV DNA From these, 25 (36%) were found positive for HBV DNA by PCR in the core region Two of these 25 sera were HBsAg positive, indicating an overt infection Of the remaining ... over 15 years of age [2] Only sera (1.3%) were positive for HBsAg [2] These sera were negative for anti-HBc antibodies A subset of 70 sera was analyzed for the presence of HBV DNA Of these, 25 (36%) ... However, the number of samples analyzed and the short genomic sequence available for study did not allow testing of this hypothesis There is accumulating evidence of a pathogenic role for OBI...
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Báo cáo sinh học: "Future perspectives in melanoma research. Meeting report from the "Melanoma Research: a bridge " doc
Ngày tải lên :
18/06/2014, 19:20
... trials and the absence of any predictive markers for the few existing therapeutic agents Adjuvant therapy of melanoma is the setting that may lend itself to the improvement of treatment given the series ... Regulation of the re-emergence of Nodal signaling in tumor cells is one of the possible molecular mechanisms underlying reversion of the metastatic phenotype To better characterize the role of Nodal, the ... be candidates for sentinel lymph node biopsy or adjuvant therapy and may also represent possible novel targets for the molecular therapy of melanoma Biomarkers In Melanoma The hypothesis that cancer...
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Báo cáo sinh học: "Interferon signaling patterns in peripheral blood lymphocytes may predict clinical outcome after high-dose interferon therapy in melanoma patients" potx
Ngày tải lên :
18/06/2014, 19:20
... unstimulated samples and fold changes were determined by dividing the MFI of stimulated samples by the MFI of the corresponding unstimulated samples Basal levels of STAT1Y701 were determined by the MFI ... CA121973 from the National Cancer Institute The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National ... with IFN [15] This offered a possible mechanism for the beneficial effect of HDI therapy in melanoma patients, and also suggested a way to select patients for therapy based on their PBL IFN signaling...
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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
Ngày tải lên :
18/06/2014, 19:20
... suppressor of malignant melanoma cell growth, further promoting evidence of expanding the testing of the role of AMPK activation for melanoma treatment Metformin is a derivative of guanidine, one of the ... and AR planned the research FN performed the majority of the experiments She received help from EvE, NA and DG on the performance of Western Blot analysis, from DG on the use of metformin, from ... sensitivity to the combination there was evidence of a pERK decrease after exposure to metformin Therefore, it is possible that the synergistic effects of vemurafenib and metformin in these cell...
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Báo cáo sinh học: "Immunological abnormalities as potential biomarkers in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis" ppt
Ngày tải lên :
18/06/2014, 19:20
... consistent finding [13-16] The functional capacity of other immune cells, such as T cells, and the contribution of other molecules in the pathophysiological mechanism of CFS/ME, remains to be determined ... were rejected because they did not meet the inclusion criteria for CFS/ME (Figure 1) 58.2% of CFS/ME patients indicated that they experienced or more of the symptoms listed in the CDC criteria list ... K were decreased only in the NK cells of the CFS/ME group compared to the healthy controls as shown in Figure 3A and 3B Altered NK Profiles in CFS/ME For the purposes of this study NK phenotypes...
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Báo cáo sinh học: " Biomarkers in T cell therapy clinical trials" potx
Ngày tải lên :
18/06/2014, 22:20
... functional for treatment to be efficacious Consequently, T cell therapy trials require the development and evaluation of additional classes of biomarkers that describe the biological properties of the ... immune responses to the infused product A summary of the classes of T cell biomarkers together with types of established assays for each class as well as advantages and disadvantages for each assay ... detail the phenotype Page of and function of the persisting T cell population, as well as the fact that this approach does not provide information about the expression status and function of the...
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báo cáo hóa học:" Interferon signaling patterns in peripheral blood lymphocytes may predict clinical outcome after high-dose interferon therapy in melanoma patients" pptx
Ngày tải lên :
20/06/2014, 03:20
... unstimulated samples and fold changes were determined by dividing the MFI of stimulated samples by the MFI of the corresponding unstimulated samples Basal levels of STAT1Y701 were determined by the MFI ... CA121973 from the National Cancer Institute The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National ... with IFN [15] This offered a possible mechanism for the beneficial effect of HDI therapy in melanoma patients, and also suggested a way to select patients for therapy based on their PBL IFN signaling...
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báo cáo hóa học:" Immunological abnormalities as potential biomarkers in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis" potx
Ngày tải lên :
20/06/2014, 03:20
... consistent finding [13-16] The functional capacity of other immune cells, such as T cells, and the contribution of other molecules in the pathophysiological mechanism of CFS/ME, remains to be determined ... were rejected because they did not meet the inclusion criteria for CFS/ME (Figure 1) 58.2% of CFS/ME patients indicated that they experienced or more of the symptoms listed in the CDC criteria list ... K were decreased only in the NK cells of the CFS/ME group compared to the healthy controls as shown in Figure 3A and 3B Altered NK Profiles in CFS/ME For the purposes of this study NK phenotypes...
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