Báo cáo Y học: Two different E2F6 proteins generated by alternative splicing and internal translation initiation ppt
Báo cáo Y học: Two different E2F6 proteins generated by alternative splicing and internal translation initiation ppt
... efficient translation initiation by ribosomal scanningfor both E2F6b and E2F6, and suggest that their translation may be initiated internally. Two different E2F6 proteins accumulate in cellsIf translation ... inhibited cells. E2F6 and E2F6b expression was again analyzed by RT-PCR withFig. 5. Expression of E2F6 and E2F6b. (A)ExpressionofE2F 6and E2F6b in primary mouse tissues was analyzed by RT-PCR withprimers ... Two different E2F6 proteins generated by alternative splicing and internal translation initiation Tillman Dahme1, Jason Wood1, David M. Livingston1 and Stefan Gaubatz21Dana...
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Báo cáo Y học: Two GPX-like proteins from Lycopersicon esculentum and Helianthus annuus are antioxidant enzymes with phospholipid hydroperoxide glutathione peroxidase and thioredoxin peroxidase activities pptx
... selenocysteine in mammalGPXs. The selenocysteine residue is important for thecatalytic activity of GPX as replacement of selenocysteine by cysteine greatly reduces the activity of the enzyme [12].According ... efficiency and substrate affinity(Tables 1 and 3). Furthermore, both enzymes were able toreduce hydrogen peroxide, as well as linoleic acid, phos-phatidylcholine dilinoleoyl and t-butyl hydroperoxides,using ... and GPXha2 proteins expressed in E. c oli cells and purified by Ni-nitrilo-triacetic acid affinity. Each crude extract (10 lgofprotein)andpurifiedrecombinant enzyme (1 lgofprotein)wereanalyzedby15%SDS/PAGE.Lane1,pET/GPXle1-transformedE....
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Báo cáo Y học: When the embryonic genome flexes its muscles Chromatin and myogenic transcription regulation ppt
... alsobeyond the induction of these two master regulatory genes.Once expressed, MyoD and Myf5 protein will drivemyoblasts toward cell cycle exit and promote terminaldifferentiation by activating ... thenucleosomes at the regulatory sites of the myogenin and MCK genes, which are bound by MyoD and MEF-2 family members, shift from a hypo- to a hyper-acteylated stateduring muscle differentiation [51]. ... lysozyme gene in myeloid cells [67], and forthe b-globin gene in erythrocytes [68]. Priming by SWI/SNFcomplexes may even apply to the myogenic determinationgenes themselves. It was recently...
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Báo cáo y học: "Two-year Outcome of Turkish Patients Treated with Zotarolimus Versus Paclitaxel Eluting Stents in an Unselected Population with Coronary Artery Disease in the Real World: A Prospective Non-randomized Registry in Southern Turk
... registry and prospectively analyzed data on a consecutive series of all patients who presented to our institution with symptomatic coronary artery disease between February 2005 and March 2007 and ... approximately two years. The primary end-point was major cardiac events, and the secondary end-point was definite stent thrombosis. Informed consent was obtained from all subjects, and the study protocol ... that the ZES is safe and reduces the rates of clinical and angiographic restenosis in patients with symptomatic coronary ar-tery disease (CAD; 4). Also the safety and efficacy of Paclitaxel-eluting...
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Báo cáo y học: " Two-stage procedure in the treatment of late chronic hip infections spacer"
... conventionally have to fulfil the criteria estab-lished by Murray [24] including: antibiotic safety, thermostability, hypoallergenicity, water solubility, adequate bactericidal spectrum and availability ... recumbency are also avoided due to early mobilisation [33]. Two- stage revision arthroplasty using ALC but without long-term systemic antibiotic therapy has also been reported by Stockley et al ... with systemic safety. The most commonly used antibiotics in ALC include tobramycin, gentamicin and vanco-mycin [26]. The combination of vancomycin and one of the aminoglycosides provides...
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Báo cáo Y học: Two independent, light-sensing two-component systems in a filamentous cyanobacterium pot
... complexing of CphB toPCB, respectively; PCB, phycocyanobilin; Pr and Pfr,redandfar-redabsorbing forms of phytochrome, respectively; Synechocystis sp.,Synechocystis sp. strain PCC 6803.Note: ... Talonmetal affinity resin (Clontech). The analysis of proteincontent and purity was performed by polyacrylamide gelelectrophoresis and Western blotting (PHAST system,Pharmacia) employing an anti ... the plant phytochromes,phytochromobilin, and to the closely related phycocyanobi-lin (PCB). Moreover, the assembled chromoproteins can becycled between Pr and Pfrforms in a way reminiscent...
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Báo cáo Y học: Two 1 : 1 binding modes for distamycin in the minor groove of d(GGCCAATTGG) docx
... d(ICICICIC)–dista-mycin [7] and later by side -by- side complexes of dista-mycin with natural targets d(ICITACIC), d(ICATATIC) and d(GTATATAC) [8,9]. Owing to the overlap of about75%, the two staggered ... amidinium end and bases A15, T8 and G9, and (B) theformamide end and bases C4 and G19. Intermolecular geometry frozen according to the crystal data. Drug fragment atoms are yellow.2874 K. Uytterhoeven ... distamycin moleculesspan almost eight base pairs and are kept together by dipole–dipole interactions between stacking pyrrole rings and amide bonds. Each drug hydrogen-bonds with thebases of only...
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Báo cáo khoa học: Two different types of hepcidins from the Japanese flounder Paralichthys olivaceus ppt
... Two different types of hepcidins from the Japaneseflounder Paralichthys olivaceusIkuo Hirono, Jee-Youn Hwang, Yasuhito Ono, Tomofumi Kurobe, Tsuyoshi Ohira, Reiko Nozaki and Takashi ... ParalichthysolivaceusCorrespondenceTakashi Aoki, Laboratory of GenomeScience, Graduate School of Marine Science and Technology, Tokyo University of MarineScience and Technology, Konan 4-5-7,Minato-ku, ... eight cysteine residues, while type II hassix [11]. In the latter type, the first cysteine residue isdeleted and the third cysteine residue is replaced with aglycine residue. The number and positions...
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Báo cáo Y học: Investigations into the mechanisms used by the C-terminal anchors of Escherichia coli penicillin-binding proteins 4, 5, 6 and 6b for membrane interaction ppt
... daphoenix@uclan.ac.ukAbbreviations:Myr2-PGro, dimyristoylglycerophosphoglycerol;Myr2-PCho, dimyristoylglycerophosphocholine; Myr2-PEtn,dimyristoylglycerophosphoethanolamine; FTIR, Fourier transforminfrared spectroscopy; ... either: dimyristoylphosphatidylcholine (Myr2-PCho), dimyristoyl phosphatidylethanol-amine (Myr2-PEtn), or dimyristoyl phosphatidylglycerol (Myr2-PGro). For spectra produced (Figs 2 and 5), ... 2002MaterialsThe peptides P4 and P5 (Table 1) were supplied by PEPSYN, University of Liverpool, UK, produced by solidstate synthesis and purified by HPLC to a purity of greaterthan 99%. The peptides...
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Báo cáo y học: "The epidemiology of intensive care unit-acquired hyponatraemia and hypernatraemia in medical-surgical intensive care unit"
... University of Calgary, Calgary, AB T2N 2T9, Canada3Department of Medicine, University of Calgary, Calgary, AB T2N 2T9, Canada4Alberta Kidney Disease Network, Calgary, AB T2N 2T9, Canada5Calgary ... validated by nursing or respiratory therapy staff onat least an hourly basis by examining the degree to which theyare representative and plausible. An HL-7 interface with theregional laboratory information ... respectively.Among patients with sodium disturbances, the mediannumber of days of hyponatraemia (IQR = one to three days) and hypernatraemia (IQR = one to five days) was two. Multivariable analysis...
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