Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx
Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx
... regulatory protein-1 complex by ATP binding and hydrolysis Zvezdana Popovic and Douglas M. TempletonLaboratory Medicine and Pathobiology, University of Toronto, CanadaMechanisms of post-transcriptional ... (GraphPad Software, San Diego, CA).AcknowledgementsThis work was supported by grants from the Heart and Stroke Foundation of Canada (grant T4134) and the Canadian Institutes of Health Research (grantMT11270). ... exposed to film, and each spot was cut out and counted by liquid scintillation. ATP hydrolysis and TLC ATP hydrolysis was determined by measuring the release of [32P]phosphate from [32P ]ATP[ cP] (Perkin...
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Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
... interaction between ETA and cyto-solic EF-2 on the one hand, and the mitochondrialmembrane on the other hand. However, the potentialrole (if any) of ADP-ribosylation of EF-2 in themitochondrial ... absence of ATP, the substrate of the vacuolarH+-ATPase pump (Fig. 3A). Immunoblot analysesshowed a progressive loss of intact ETA and ETA-Ain the presence of ATP, with concomitant generation of ... reducing SDS ⁄ PAGE followed by western blotting to determine the endosomal content and integrity of ETA and ETA-A.To specifically assess the membrane translocation of intact and processed ETA through...
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Báo cáo khoa học: Characterization of the Drosophila Methoprene -tolerant gene product Juvenile hormone binding and ligand-dependent gene regulation potx
... implication of another class of transcriptional regulator in JH signaling. Wilson and coworkers examined the resistance mechanism of Dro-sophila to the JHA, methoprene, and isolated mutantlines of flies ... having overhangs of EcoRV and XbaI sites was prepared by PCR and subcloned intopBIND vector (Promega); the cDNA fragment containingthe fused ORF of GAL4–DBD and Met was amplified by PCR and subcloned ... multimeric complex including hsp90anchors the unliganded Ahr in the cytoplasm, therebypreventing its transactivation function [36]. Upon lig- and binding, Ahr translocates to the nucleus and formsa...
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Tài liệu Báo cáo khoa học: Inhibition of cobalamin-dependent methionine synthase by substituted benzo-fused heterocycles pptx
... Shi Q, Zhang Z, Li G, Pillow PC, Hernandez LM, SpitzMR & Wei Q (2005) Polymorphisms of methioninesynthase and methionine synthase reductase and risk of lung cancer: a case-control analysis. ... reactions of trans-sulfura-tion through the production of homocysteine, biologi-cal methylations of DNA, lipids and proteins, and polyamine biosynthesis through the production of methionine and S-AdoMet ... optimization. Ligands were parameter-ized using partial atomic charges and other valuesobtained from quantum mechanic modelling (Hartree-Fock 6–31G*) of the ligand structure using pc spartanpro (Wavefunction...
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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx
... Con-formational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands:s, d, 2.5 and 20 mM choline; n, m, 2.5 mM and 20 mM atropine;h, ... other hand,ipratropium and atropine also induced the appearance of these negative bands, although with reduced intensi-ties and with a much lower concentration of ligand, asthe ellipticity change ... viability and liberation of viru-lence factors. Inhibition of autolysis by excess cho-line might, in the first instance, seem to be of therapeutic interest. However, the amount of ligandneeded, and...
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Tài liệu Báo cáo khoa học: Inhibition of pea ferredoxin–NADP(H) reductase by Zn-ferrocyanide docx
... [51,52].The O of Ser90 and the S of Cys266 of pea FNR are closeto N5 and O4 of the isoalloxazine, which are involved inhydride transfer. The hydroxy group of Ser90 could accept ahydrogen bond and thus ... (0, 1.5, 3 and 5 lM), at differentconcentrations of NADPH (5, 10, 25, 50, 100 and 300 lM),DCPIP (5, 10, 15, 25, 35 and 50 lM), ferricyanide (100, 250,500, 750 and 1000 lM) and Fd (10, ... mainlycauses enzyme inhibition by Zn-ferrocyanide.Effect of Fld on the inhibition of FNR activities by Zn-ferrocyanideIn some photosynthetic systems, such as that of certainalgae and cyanobacteria,...
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Tài liệu Báo cáo khoa học: Expression of an a-1,3-glucanase during mycoparasitic interaction of Trichoderma asperellum docx
... (a-1,3- and a-1,6-glu-can), nigeran (a-1,3- and a-1,4-glucan), soluble starch(a-1,4- and a-1,6-glucan), pustulan (b-1,6-glucan), laminarin(b-1,3-glucan), carboxymethilcelullose (b-1,4-glucan) ... resistancein plants [3], their potential for promoting plantgrowth [4] and their antagonistic activity against awide range of plant pathogenic fungi [5]. The inhibi-tory effect of their antibiotics ... MALDI-TOF analysis and I. Chamorrofor the technical assistance.References1 Howell CR (2003) Mechanisms employed by Trichodermaspecies in the biological control of plant diseases: the his-tory and...
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Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx
... being more sensitive thanGR and LDH, and inactivation of G APDH being metal-ion independent, whereas inhibition of GR and LDH by H2O2occurs most rapidly in the presence of metal ions.It is ... peroxides a re alsoTable 1. Inhibition of glutathione reductase by H2O2 and N-Ac-Trp-OMe peroxides, and lactate dehydrogenase by H2O2, in the presence and absence of added Fe2+–EDTA. Samples ... Oxidation of high density lipopro-teins. I. Formation of methionine sulfoxide in apolipoproteins AI and AII is an early event that accompanies lipid peroxidation and can be enhanced by alpha-tocopherol....
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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf
... expression of Akt and PrPCwas examined by immunohistochemistry and confocal microscope in human gastric cancer cases and cell lines. Expression of total and p-Akt was detected incells transfected ... resistance in gastric cancer cellsJie Liang*, Fulin Ge*, Changcun Guo, Guanhong Luo, Xin Wang, Guohong Han, Dexin Zhang,Jianhong Wang, Kai Li, Yanglin Pan, Liping Yao, Zhanxin Yin, Xuegang Guo, Kaichun ... accumulation and increasing release of these drugs (ADR and VCR) [5].PrPC-mediated multidrug resistance (MDR) wasthought to be related to upregulation of P-glycoprotein(P-gp) [6] and inhibition of...
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Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx
... of Ca2+(lanes 5, 6 and 9, 10). In the absence of Ca2+(lanes 3, 4 and 7, 8) and for the GST control (lanes 1 and 2) there was no retention of hCaM detectable.Table 1. Binding of MCGS-(H)6-hCaM ... channels by Ca2+⁄ CaM. Employment of CaM-EF-hand mutants showed that CaM binding to the N- and C-ter-minus are primarily dependent on EF-hand motifs 3 and 4. Hence, closure of EAG channels presumably ... threshold(Fig. 2) and by assuming that a CaM binding domainhas to have a length of at least around 15 residues.For quantitative estimates of the binding of CaM and CaM mutants to peptides and fusion...
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Từ khóa: Nghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ