... threedomain E3-binding protein (E3BP, Fig. 1) also has ananchoring C-terminal domain, an E3-binding domain and amobile lipoyl domain. Again, the lipoyl domain contributesas a substrate and intermediate ... subunitdomains: L1, N-terminal lipoyl domain; L2, inner lipoyl domain; B, E1binding domain; I, oligomer-forming, acetyl-transferase-catalyzinginner domain. E3BP domains: L3, N-terminal lipoyl domain; ... PDP1 for binding to L2. In a second region at the other end of the L2 domain,mutation of glutamates 16 2, 17 9 and 18 2, and glutamine 18 1 greatly reduces binding. Indeed, substitution of alanineor...