... support of this hypo-thesis are meager. In the head-to-tail orientation, the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the ... peak, whereas the elution of the low-affinity peak was unchanged.To investigate the effect of substitutions in the tryptophancluster of the C-terminal domain on heparin affinity, the W393A/W394A ... (Arg405, Lys407,Lys413, Lys414, Lys422, Lys428, and Lys430) oriented in the same direction with the heparin-binding domain at the N-terminal end [14]. The other is a cluster of tryptophans(Trp390,...