Tài liệu Báo cáo Y học: The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex potx
Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf
... a density of 6.0 · 105per 100 mmdish for 2 days and then assayed for JNK activity. JNKassays were performed by using a SAPK ⁄ JNK Assay kit(Cell Signaling Technology) according to the manufac-turer’s ... Kit-8 is a colorimetric method in which the intensity of the dye is proportional to the number of the viable cells. Brie y, 200 lL of a suspension of PC-9cells was seeded into each well of a ... determined by both immunoblot analysis for levels of phos-phorylated JNK and an in vitro activity assay. Various types of stimuliactivated JNK through phosphorylation by the dual-specificity JNKkinases,...
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Tài liệu Báo cáo Y học: The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex potx
... The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex Birgitta Tomkinson, Bairbre Nı´Laoi and Kimberly WellingtonDepartment ... ascribed to the insert between Asp and His of the catalytic domain of TPP II. We can now report that the region surroundingArg252 is of importance for the formation of the oligomericenzyme complex, ... if the a ctive complexes were assembled from the two types of homodimers. The insert within the catalytic domain is of importance for complex formation No functional significance has previously...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... model for the IICBGlcdimer of EIIGlc. IICBGlccon-sists of a membrane-spanning C domain (grey) and the cytoplasmicIIB domain (black). IICBGlc is phosphorylated at Cys421 by the sol-uble IIAGlcsubunit. ... wererecently introduced for the preparation of C-6 aldehydederivatives of Glc [15]. The key step is the use of Collinsreagent for the selective oxidation of the primary trimethyl-silyl ether of the ... stereoselective synthesis of enantiomericallypure mono- and di-O-hexadecyl-b-D-glucosylglycerol ethers byepoxidation of an allyl b-D-glucopyranoside asymmetricallyinduced by the glucide moiety. Tetrahedron:...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... Expression of lacA is also detected in the presence of these compounds, but in the presence of D-xyloseand xylan as well. This can be explained by the structure of the polysaccharides. Xylan contains ... galacto-oligosaccharides.ACKNOWLEDGEMENTS The authors thank Simon Flitter for the identification and isolation of the galA containing phage clones and Matthew Illsley for the analysis of a-L-1,5-arabinofuranosidase activity in the ... chains of pectin. This is in agreement with aprevious study in which the synergy of enzymes degrading the pectin side chains was studied [34]. GALA had a positiveeffect on the activity of the other...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... apoenzymes of eachtype of SOD may be reconstituted by the addition of metals, the resulting enzyme is active only with the authentic metal atits active centre [4–7]. A small number of cambialistic ... on enzyme activityHydrogen peroxide, the product of the SOD reaction, is an inhibitor of SODs and an inactivator of FeSOD. It is often used to distinguish between the two types of isoenzyme, ... levels of hydrogen peroxide has therefore been used as anindicative marker for the presence of iron at the active siteand, indeed, is frequently used to determine the type of SODisoenzyme present...
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Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
... polysaccharide [43]. The role of Cj1428c in Campylobacter polysaccharide biosynthesis is currently unknown. There is no evidence for the presence of fucose in any carbohydrate structures of NCTC ... we are currently investigating thispossibility. Alternatively, this enzyme may be involved in the epimerization reaction necessary for the biosynthesis of the capsular heptopyranose as alditol ... characterization of specific enzymes thatare involved in the biosynthesis of these structures and maylead to the discovery of potential therapeutic targets. Inaddition, the correlation of carbohydrate...
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Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field pptx
... asreported previously [20]. The serine, threonine and tyrosinecontents of the hydrolysates were corrected for destructionduring the hydrolysis by extrapolation to zero time hydro-lysis. The amino acid ... amide III).Most of the absorption of the later band came within the region 1390 ± 40 cm)1, in which the methyl or methy-lene deformations are also active. The WSM sampleabsorbed less in the ... paper presents for the first time the results of acomparative study on the organic matrix extracted from the nacreous layer of the shell from the pearl oysterPinctada maxima by two very different...
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Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx
... the thiyl radical of the lipoyl residue should efficiently interactwith the E1 catalytic intermediate, as the lipoyl-bearing domain of E2 is designed for this interaction (reaction 2). Inthis ... substratemoiety during the complex- catalyzed succinyl-CoA hydro-lysis [72] accounts for the inactivation by dihydrolipoamidein the presence of succinyl-CoA (Table 2).DISCUSSIONGeneration of radical ... mechanisms of the inactivation of the complex by the thiyl radical of dihydrolipoate, we tookinto account that (a) the overall activity (Fig. 2) is decreaseddue to the irreversible inactivation of...
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Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations influence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx
... putative therapeutic agents for these disabling diseases.Keywords: amyloidosis; keratoepithelin; lattice corneal dys-trophy; granular corneal dystrophy; synthetic peptide.Hereditary corneal dystrophies ... and severely impair visualacuity in adulthood. Their therapy is restricted to keratopl-asty and phototherapeutic keratectomy by Excimer laser.Unfortunately, the benefits of these therapies remaintransient ... and by 24 h.Role of the Cys–Cys disulfide bonds The role of disulfide bonding was examined by comparingamyloid formation of C110)131 to that of the same peptidewith blocked sulfated radicals of...
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Tài liệu Báo cáo Y học: The RuvABC resolvasome Quantitative analysis of RuvA and RuvC assembly on junction DNA doc
... analysis of the RuvAC–Holliday junction ternary complex To further examine the hypothesis that a tetramer of RuvAcan bind to each face of a Holliday junction, the effect of the addition of RuvC ... RmaxRuvA is the maximum response of RuvAbinding, Rjunc is the response of the binding of the biotinylated Holliday junction, MrRuvA is the molecularmass of RuvA and Mr junc is the molecular ... quantified the interaction of the RuvA protein with synthetic Holliday junctions andhave shown that the binding of the protein is highly struc-ture-specific, and leads to the formation of a complex...
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Từ khóa: tài liệu báo cáo khoa họctài liệu báo cáo nghiên cứu khoa họctai lieu bao cao thuc tap y si da khoatai lieu bao cao thuc tap tim hieu nhan cach mot hoc sinhtai lieu bao cao thuc tap nganh the ducbáo cáo y họcBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roTranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM