Tài liệu Báo cáo Y học: Human and Drosophila UDP-galactose transporters transport UDP-N-acetylgalactosamine in addition to UDP-galactose doc
Tài liệu Báo cáo Y học: Human and Drosophila UDP-galactose transporters transport UDP-N-acetylgalactosamine in addition to UDP-galactose doc
... 2002 Human and Drosophila UDP-galactose transporters transport UDP-N-acetylgalactosamine in addition to UDP-galactose Hiroaki Segawa*, Masao Kawakita and Nobuhiro IshidaDepartment of Physiological ... detected in a position and stage-speci®c manner during Drosophila development [24].Heparin-like glycosaminoglycans that contain galactose and N-acetylgalactosamine residues are involved in the winglesssignaling ... USA) and the radioactivity retained on the ®lterwas measured.Staining with lectin and antibodyLectin staining and indirect immuno¯uorescence stainingwere carried out as described previously...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... 2-arachidonoylglycerol (2-AG), the cannabinoid CB1 and CB2receptors, and one of the enzymes mostlyresponsible for endocannabinoid hydrolysis, the fatty acidamide hydrolase (FAAH). By using a very ... Parkinson’s disease) and pain [1], and to play adown-regulatory role on the immune system [2]. Indeed,cannabinoids exhibit immunosuppressive properties and in vitro they weaken humoral immunity ... described in McIntosh et al. [41] and in Shire et al.[42],respectively. Rabbit anti -human and rat FAAH polyclonalantibody, kindly provided by M. Maccarrone (Departmentof Experimental Medicine and...
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Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx
... amino acid-rich media included: YPD [yeast extract and Bactopeptone(YP) containing 2% dextrose]; YPA (YP containing 0.05%glucose and 2% potassium acetate); and YPG (YPcontaining 3.5% galactose).Phospholipase ... PtdEtn-PLDactivity in response to inositol and/ or choline in the medium. Wild-type and spo14D mutantstrains were grown to mid log-phase in SCmedium in the absence or presence of choline(1 mM) and inositol ... proteins including protein kinase C, cytosolicphospholipase A2 and synaptotagmin [33]. We have there-fore screened the yeast genome and identified three ORFsencoding hypothetical unknown proteins...
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Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf
... process in mammals during late embryogenesis [6,7]. The methylationpattern is created by de novo methylation and demethyla-tion of the DNA, and maintained during mitosis bymaintenance methylation ... groups are always introduced, oneslowly by Dnmt3a or by de novo methylation catalyzed byDnmt1 and the second one very fast. Therefore, this effectcan only double the rate of DNA methylation, becauseevery ... binding of methylated DNA to an allostericsite located in the N-terminal domain of the enzyme[18,19]. However, de novo methylation activity is alsoassociated to the Dnmt3a and Dnmt3b enzymes, whichdo...
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Tài liệu Báo cáo Y học: DNA and RNA damage by Cu(II)-amikacin complex docx
... other amino-glycosides, as kanamycin A, neomycin B and a syntheticneomycin-neomycin dimer have been shown to inducefragmentation of tRNAPheat Y3 7 and additionally atm7G46 in the variable ... were observed and they disappeared after removingwybutine. Interestingly, enhancement of wybutine fluores-cence was observed after addition of neomycin B orkanamycin A to tRNAPhe and the effect ... cleavage.Amikacin (Ami) is a semisynthetic aminoglycoside, aderivative of kanamycin A, having the B1 amino group of2-deoxystreptamine moiety modified by acylation with4-amino-2-hydroxybutyric acid. Previous...
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Tài liệu Báo cáo Y học: A Raman optical activity study of rheomorphism in caseins, synucleins and tau New insight into the structure and behaviour of natively unfolded proteins pot
... [6], prothymosina [7], a bacterial ®bronectin-binding protein [8], the brainprotein a-synuclein together with the related proteinsb-synuclein and c-synuclein [9±12], a nd the brain proteintau ... residues, asobserved in the caseins [3], synucleins [10], tau [14],prothymosin a [7] and the ®bronectin-binding protein [8], and also in typical chemically denatured proteins [56±58].Over extensive ... A Raman optical activity study of rheomorphism in caseins,synucleins and tauNew insight into the structure and behaviour of natively unfolded proteinsChristopher D. Syme1, Ewan W. Blanch1,...
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Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
... might in uenceantibacterial activity that differ between opistoporin 1 and pandinin 1 are the hydrophobicity (pandinin 1–0.1214,opistoporin 1–0.1652) and the hydrophobic moment, whichis nearly ... recently described in the venom of spiders and insects.Parabutoporin and opistoporin 1 were synthesizedchemically and preliminary studies on antibacterial activityshowed that the quality and ... protein synthesis by binding to 50Sribosomal subunits of sensitive microorganisms and it has to pass both inner and outer membranes to be active.Synergy of cationic peptides with erythromycin...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... atom. Tyr183 isbelieved to assist Asp125 in this function by hydrogenbonding to the carbonyl oxygen of the N-acetyl group. In this specific orien tation the N- acetyl carbonyl oxygenatom is in ... classes.Role of Tyr183 in catalysis In previous crystallographic studies it was shown that thehydroxyl side chain of Tyr183 is within hydrogen bondingdistance of the N-acetyl carbonyl oxygen of the ... rotated away from the C1 atom of t hat residue.The c ombined s tructural and k inetic data sh ow thatAsp125 and Tyr183 contribute to catalysis by positioningthe carbonyl oxygen of the N-acetyl group...
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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf
... full-length proteins.Keywords: calretinin; calcium; calbindin D28k; EF-hand;NMR secondary structure.Calretinin (CR) and calbindin D28k(Calb) are homologouscalcium-binding EF-hand proteins with ... C.P.(1999) A kinetic and stereochemical investigation of the role oflysine-32 in the phenylpyruvate tautomerase activity catalyzed bymacrophage migration inhibitory factor. Biochemistry 38,16024–16033.44. ... changes in calbindin-D28K(the vitaminD-induced calcium binding protein) interactions with chickintestinal brush border membrane alkaline phosphatase as studiedvia photoaffinity labeling techniques....
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Tài liệu Báo cáo khoa học: "Dialogue and Understanding Development Environment, mapping Business Process Models to Information State Update dialogue systeDialogue and Understanding Development Environment, mapping Business Process Modelsms" pptx
... Dialogue and Understanding Development Environment,mapping Business Process Models to Information State Update dialoguesystemsOliver Lemon and Xingkun LiuSchool of InformaticsUniversity of Edinburgholemon,xliu4 ... recognition, interpretation, and production) the human operator is responsible for:contextual interpretation of incoming spee c hmaintaining a nd updating dialogue contextdialogue strategy (e.g. ... non-experts to construct, mod-ify, and rapidly deploy process mod e ls and the result-ing interactions, including interactions with back-end99databases. For example, a manager may decide (a...
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