Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
Tài liệu Báo cáo Y học: Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis Relation to trehalose-based oligosaccharides attached to lipid docx
... allowchemical characterization of each. CO3-1 was identified astrehalose by TLC as well as by MALDI-TOF MS analysis of the products obtained by permethylation and hydrolysis,andalsoby1H-NMR.Oligosaccharide ... concentration of 70% toprecipitate glycogen and any other trichloroacetate-solublepolymers present in the cytoplasm. The alcohol mixture was allowed to stand overnight in the cold, and any precipitate ... heat shock and other adverse conditions [5,6].Trehalose (Glca1–1aGlc) is not found in any vertebrates,nor is it synthesized in these organisms. Thus, the synthesis and acylation of trehalose...
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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
... catalyzed the ring fissionbetween C2 and C3 of 4-amino-3-hydroxybenzoic acid, with the consumption of 1 mol of O2per mol of substrate. The enzyme was therefore designated as 4-amino-3-hydroxy-benzoate ... amidol,3-hydroxyanthralinic acid, 1,2,4-trihydroxybenzene and pyrogallol) in aqueous solution were always freshlyprepared and used immediately.Effect of various compounds on the enzyme activity The ... Fe2+per mol of enzyme. Addition of Fe2+increased the enzyme activity and chelating agents repressed the enzyme activity, indicating that the enzyme probablyrequires Fe2+for activity. Other extradiol...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... wererecently introduced for the preparation of C-6 aldehydederivatives of Glc [15]. The key step is the use of Collinsreagent for the selective oxidation of the primary trimethyl-silyl ether of the ... against inactivation. To the contrary, the presence of Glc slightly sensitized EIIGlcfor inactivation by the isothiocyanates 1e and 3d. On the other hand, the rate of inactivation by bromoacetyl-Glc ... G. (1986) The oxidation of primary trimethylsilyl ethers toaldehydes: a selective conversion of a primary hydroxy group intoan aldehyde group in the presence of a secondary hydroxy group.J....
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... 2 h and 20 h to determine the substratespecificity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed byHPAEC and MALDI-TOF MS. HPAEC analysis ... chains of pectin. This is in agreement with aprevious study in which the synergy of enzymes degrading the pectin side chains was studied [34]. GALA had a positiveeffect on the activity of the other ... thank Simon Flitter for the identification and isolation of the galA containing phage clones and Matthew Illsley for the analysis of a-L-1,5-arabinofuranosidase activity in the endogalactanasepreparation.REFERENCES1....
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... governed byone or even two residues, but is most likely accomplished by the concerted effects of a combination of key residues.Further analysis of these and other mutant SODs iscurrently underway.ACKNOWLEDGEMENTSWe ... in a hydrogen-bonding networkinvolving other residues and solvent molecules. An unex-pected result was the extremely high thermostability of Mn[G77Q] and the very low thermostability of Fe[A141Q](Fig. ... represents either iron or manganese.Selectivity of the proteins for their metal cofactor has beendemonstrated in vivo [3] and although apoenzymes of eachtype of SOD may be reconstituted by the addition...
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Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
... characterization of specific enzymes thatare involved in the biosynthesis of these structures and maylead to the discovery of potential therapeutic targets. Inaddition, the correlation of carbohydrate ... A.M., Wu, Y. , Young, N.M. & Wakarchuk, W.W.(2000) Biosynthesis of ganglioside mimics in Campylobacter jejuniOH4384. Identification of the glycosyltransferase genes, enzymatic synthesis of model ... established by NMR was observed in the FAB-MS and MALDI-MS (Fig. 8) of the methylated PS-2. The MALDI-MS spectra shows a series of A-type primaryglycosyl oxonium ions and secondary ions of definedcompositions...
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Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field pptx
... composition was very similar to the WSM, and consequently the EDTA-IM. Hydroxyproline, hydroxy-lysine and phosphoserine were not detected in WSM, WIM,EDTA-SM and EDTA-IM. The comparison of the amino ... Proline, hydroxyproline and hydroxylysine were examined at 254 nm by reverse-phaseHPLC of their phenylisothiocarbamate derivatives [26], asreported previously [20]. The serine, threonine and tyrosinecontents ... for the first time the results of acomparative study on the organic matrix extracted from the nacreous layer of the shell from the pearl oysterPinctada maxima by two very different methods. The...
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Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx
... appearance of the ThDP adduct with the substratemoiety during the complex-catalyzed succinyl-CoA hydro-lysis [72] accounts for the inactivation by dihydrolipoamidein the presence of succinyl-CoA ... mechanisms of the inactivation of the complex by the thiyl radical of dihydrolipoate, we tookinto account that (a) the overall activity (Fig. 2) is decreaseddue to the irreversible inactivation of ... the pyruvate dehy-drogenase multienzyme complex of Escherichia coli. Biochem. J.155, 429–432.65. Grande, H.J., Van Telgen, H.J. & Veeger, C. (1976) Symmetry and asymmetry of the pyruvate...
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Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations influence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx
... decades of life and severely impair visualacuity in adulthood. Their therapy is restricted to keratopl-asty and phototherapeutic keratectomy by Excimer laser.Unfortunately, the benefits of these therapies ... in a phenotypic variant of granular cornealdystrophy type 1 (GCD1), Arg124His (R124H) [1] ingranular corneal dystrophy type 2 (GCD2, Avellinodystrophy) a mixed type of amyloid and granular ... did notmodify amyloid yielding.DISCUSSIONWe previously presented an effective dialysis-based in vitro system to study the formation of amyloid fibrils fromTGFBI protein-related synthetic peptides...
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Tài liệu Báo cáo Y học: The RuvABC resolvasome Quantitative analysis of RuvA and RuvC assembly on junction DNA doc
... purification of oligodeoxynucleotidesused in the binding studies Oligonucleotide synthesis was performed on an AppliedBiosystems 394 DNA synthesiser using cyanoethyl phos-phoramidite chemistry. The ... Also,E. coli RuvA has the ability to bind to the duplex arms of the Holliday junctions after specifically binding to the Holliday junction at the crossover point.From the analysis of the equilibrium ... examine the hypothesis that a tetramer of RuvAcan bind to each face of a Holliday junction, the effect of the addition of RuvC to the RuvA–Holliday junction complexwas investigated. Analysis of the...
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