... DEC1 with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for ... to those of intact BMAL1 and DEC1, respectively (Fig. 1A,C). These findings indicatethat the region between amino acids 28 and 139 of DEC1(including the bHLH domain) is sufficient for the suppressive ... genes, including Dec2 and PPARc2,intheabsence of other transcription facto rs, such as CLOCK and BMAL1 [9,10,22,23]: an N-terminal region between aminoacids 1 and 141, and another region between...