validation of biochemical markers by calcium kinetics
Báo cáo y học: "A prospective assessment of the inter-laboratory variability of biochemical markers of fibrosis (FibroTest) and activity (ActiTest) in patients with chronic liver disease" ppt
... the variability of FibroTest and ActiTest in relation to the method of expressing enzymatic activity; in particular, in terms of absolute values or as multiples of the upper limit of normal Since ... definitions of normal values may be inappropriate [8– 10], a major concern was the definition of ALT and GGT activity In routine practice, the definition of the upper limit of normal (ULN) of ALT ... observed a mean occurrence of pain in 30 % of patients, out of 1,000 endured severe adverse events, and out of 10,000 died [2] There is no ideal gold standard for the assessment of liver histology Even...
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Báo cáo y học: " Intra-individual fasting versus postprandial variation of biochemical markers of liver fibrosis (FibroTest) and activity (ActiTest)" pdf
... calories of the meal was 782 kilo-calories with a maximum of 1340 kcal, it is prudent to recommend a meal of around 800 kcal Conclusions This study shows that the biochemical markers of liver ... long-term medication use of the contraceptive pill and the ingestion of a NSAID (ibuprofen) for pelvic pain 48 hours before sampling The aim of this study was to assess the impact of meal intake on ... strongly suggest that due to the limitations [4-6] and risks of biopsy [7], as well as the improvement of the diagnostic accuracy of biochemical markers [8,9], liver biopsy should no longer be considered...
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Báo cáo y học: "Overview of the diagnostic value of biochemical markers of liver fibrosis (FibroTest, HCV FibroSure) and necrosis (ActiTest) in patients with chronic hepatitis C" doc
... details were given concerning the causes of discordance between biopsy and biochemical markers However FT-AT is the only panel of markers identified by an independent overview [9], which has ... cutoff (91%), as was the negative predictive value for excluding significant activity at the 0.36 cutoff of AT (85% negative predictive value) The positive predictive value of the 0.72 cutoff of ... cut off of 0.36, the ActiTest negative predictive value for excluding significant necrosis (prevalence 0.41) was 85% (Table 2) Comparison of FT-AT diagnostic values with other biochemical markers...
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Báo cáo y học: "Development and validation of molecular markers for characterization of Boehmeria nivea var. nivea and Boehmeria nivea var. tenacissima" pps
... Fragments of CAPs markers digested with Afl III and Nde I A CAPS profile generated by digestion with restriction enzyme Afl III Representative samples of each Boehmeria lines are shown on top of the ... 100 bp Figure Quality control of admixed DNA samples A Quality test of CAPS markers Digestion pattern with restriction enzyme Afl III of the CAPS profile generated by admixed DNA samples from both ... size of DNA standards M: DNA markers B CAPS profile generated by digestion with restriction enzyme Nde I Li et al Chinese Medicine 2010, 5:40 http://www.cmjournal.org/content/5/1/40 A Page of...
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AN INVESTIGATION INTO LINGUISTIC FEATURES OF MODALITY MARKERS USED BY TEACHERS IN LECTURES IN ENGLISH
... the coordination of the speakers and the recognised with the appearance of adjacency pairs, so it is preceded by a series of turns The control of this process is turn taking Schegloff [33] points ... the coordination of the speakers and the recognised with the appearance of adjacency pairs, so it is preceded by a series of turns The control of this process is turn taking Schegloff [33] points ... terms of the aspects mentioned in the aims and objectives In addition, because of its emphasis on in-depth knowledge and elaboration of concept as well as description of frequency of occurrence of...
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Tài liệu Báo cáo khoa học: Kinetics and thermodynamics of nick sealing by T4 DNA ligase pptx
... amplitude of the burst phase reaches % 50% of the total extent of ligation when [ATP] is taken of the same ể FEBS 2003 Kinetics of nick sealing by T4 DNA ligase (Eur J Biochem 270) 4319 order of magnitude ... of joining rates on [Mg2+] follows the changes in the equilibrium concentrations of the monomagnesium and dimagnesium forms of ATP (Fig 5B) The maximal rate of the slow phase of the joining of ... concentration of ATPMg On the other hand, the rate of the burst phase of joining of M5C19, and the joining rate of the complementary oligonucleotide C24 resembles more the increase in the concentration of...
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Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elafin and pre-elafin (trappin-2) expressed in Pichia pastoris ppt
... elan-immunoreactive material Fig Evolution of rec-trappin-2 production by Pichia pastoris as a function of the duration of fermentation Aliquots of concentrated supernatants of rec-trappin-2-secreting P pastoris ... formed by incubating equimolar concentrations (10)6 M) of enzyme and inhibitor Dissociation of the complexes was induced by dilution in a concentrated substrate (1.88 mM) solution (B) Kinetics of ... Simon, S.R (2001) Kinetics of the Inhibition of Proteinase by Elan Am J Respir Cell Mol Biol 24, 8389 25 Bieth, J.G (1995) Theoretical and practical aspects of proteinase inhibition kinetics Methods...
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Growth kinetics of silicon nanowires by platinum assisted vapour–liquid–solid mechanism
... is dependent on the type of catalyst This could be explained by the effect of the catalyst on the barrier energy to the nucleation of a new step at the growth front of the nanowires (i.e., the ... 4, by plotting the growth rate with 200 Length (µm) 150 100 50 0 10 15 Time (min) Fig Length of Si nanowires using Au and Pt versus time at holding temperature of 1000 °C Fig Construction of ... (c) HRTEM image of Si nanowire using Au (d) HRTEM image of Si nanowire using Pt Both were single crystal (e) EDS line mapping of Si nanowires using Au catalyst (f) EDS line mapping of Si nanowires...
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Báo cáo Y học: Thermodynamics and kinetics of the cleavage of DNA catalyzed by bleomycin A5 A microcalorimetric study pdf
... enzyme although the cleavage of DNA by BLM-A5 follows Michaelis–Menten kinetics The cleavage of calf thymus DNA by a mixture of ADM, Fe3+, Vc and O2 and by a mixture of (1,10phenanthroline)-copper(II), ... the catalytic efficiency (represented by k2/Km) of BLM-A5 is of the same order of magnitude as that of lysozyme but several orders of magnitude lower than those of TaqI restriction endonuclease, ... Ó FEBS 2002 Thermokinetics of DNA cleavage catalyzed by BLM-A5 (Eur J Biochem 269) 2855 Table Thermodynamic and kinetic data of the cleavage of calf thymus DNA by a mixture of BLM-A5, Fe2+ and...
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Báo cáo khoa học: Inhibition kinetics of catabolic dehydrogenases by elevated moieties of ATP and ADP – implication for a new regulation mechanism in Lactococcus lactis potx
... the ux of ATP production through strong nonlinear inhibition of ADH: by obtaining two instead of three ATPs per sugar unit in excess concentrations of ATP and ADP [1] Inhibition kinetics of dehydrogenases ... inuenced by any of the combinations of inhibitors A similar conclusion can be drawn for the combination of ADP + NAD for ADH, whereas there was a slight increase in inhibition of ADH by the combination ... type of inhibition can occur in eukaryotic ADHs, indicating that this type of inhibition might be widespread in nature Results Inhibition kinetics by a single inhibitor The inhibition kinetics of...
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handbook of biochemical kinetics a guide to dynamic processes in the molecular life sciences
... Handbook of Biochemical Kinetics This Page Intentionally Left Blank Handbook of Biochemical Kinetics Daniel L Purich R Donald Allison Department of Biochemistry and Molecular Biology University of ... (i.e., the absorption of light by one chemical species is unaffected Handbook of Biochemical Kinetics Absorption Spectroscopy by the presence of other species, irrespective of whether those other ... realm of biochemical kinetics and certainly not within a first edition We are also certain that, despite a determined effort to cover the terminology of chemical Handbook of Biochemical Kinetics...
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Báo cáo hóa học: " Optimization, Yield Studies and Morphology of WO3 Nano-Wires Synthesized by Laser Pyrolysis in C2H2 and O2 Ambients— Validation of a New Growth Mechanism" pdf
... schematic of the solid–vapour–solid mechanism of growth of 1D nano-structure from a spherical layer of atoms in a tip growth 123 This corresponds to the number of atoms in the ith segment of the rod of ... CuKa wavelength of 0.154184 nm The experimental procedure showed good reproducibility of results Lengths and corresponding diameters of the nano-wires were measured by means of a software package ... assembled into the rod of length l1, the next layer in the sphere has a radius of R0-X which forms the next segment of the rod of length l2 The subsequent layers have radii of R0 - 2X, R0 - 3X,...
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Handbook of Biochemical Kinetics pptx
... Handbook of Biochemical Kinetics This Page Intentionally Left Blank Handbook of Biochemical Kinetics Daniel L Purich R Donald Allison Department of Biochemistry and Molecular Biology University of ... (i.e., the absorption of light by one chemical species is unaffected Handbook of Biochemical Kinetics Absorption Spectroscopy by the presence of other species, irrespective of whether those other ... realm of biochemical kinetics and certainly not within a first edition We are also certain that, despite a determined effort to cover the terminology of chemical Handbook of Biochemical Kinetics...
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Báo cáo y học: "Relationship between radiographic grading of osteoarthritis and the biochemical markers for arthritis in knee osteoarthritis" doc
... mostly women and characterized by the development of Heberden nodes and a specific pattern of OA showing multiple joint involvement Because levels of serum biochemical markers for arthritis depend ... metalloproteinase; TIMP, tissue inhibitor of metalloproteinases biochemical markers of bone metabolism are also proposed as indicators of disease progress of OA [14,15] Spector and colleagues demonstrated ... inhibitor of metalloproteinases There are two major categories of substances that are currently being investigated as potential biochemical markers for arthritis One includes constituents of the...
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Báo cáo lâm nghiệp: "Ultrastructural and biochemical changes at the preinfection stage of mycorrhizal formation by two isolates of Pisolithus tinctorius" ppt
... comparison of the early events of infection between E urophylla and both strains of P tinctorius shows important differences at the interface Indeed, it seems that the plant reacts to the presence of ... controlled utilization of mycorrhizal symbionts in plantation man- agement not have any knowthe nature of the signal from each organism, which initiates this succession of early events of mycorrhizal ... Lapeyrie F & Garbaye J (1989) Infectivity of two isolates of Pisolithus tincto- rius isolated from beneath pine and eucalypts respectively on roots of Eucalyptus urophylla in vitro Mycorrhizal...
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Báo cáo y học: "Biochemical markers of bone turnover and their association with bone marrow lesions" docx
... Heart Study Offspring cohort included surviving descendants (and spouses of descendants) of the original Framingham Heart Study cohort [25] As part of a study of the inheritance of OA, selected ... are some limitations of this work that warrant mention, some of which are generic to the application of biomarkers Age-related increases are commonly seen in biochemical markers, and these may ... for these analyses Bone biomarkers Biochemical markers of bone turnover were assayed in archived serum and urine stored at -70°C We measured two serum bone formation markers: osteocalcin and bone-specific...
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báo cáo khoa học: " Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L.) Millspaugh]" pptx
... portion of the genome plays a major role in the evolution of species [54] This is the first report of development and validation of a comprehensive set of genic-SSR markers in pigeonpea by deep ... of 20-25 bases with an optimum of 22 bases; PCR product size range of 100-200 bp; optimum annealing temperature of 50-60°C; GC content of 40-60% with an optimum of 50%; the specified number of ... were compared by BLASTX tool of NCBI at a cutoff bit score of 50 against the non-redundant protein database SSR marker scoring and data analysis The genotype profiles produced by SSR markers were...
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Báo cáo y học: "Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2" ppsx
... stopped with 50 μL of 2N H2SO4, and optical density (OD) was read at 490 nm Confirmation of negative-positive cutoff The negative-positive cutoff value was set by the average OD ratio of 25 field negative ... average OD of positive sera tested on GST alone was not significant different from that of negative sera tested on GST alone (P > 0.05) Confirmation of negative-positive cutoff A cutoff point ... coefficient of variation (CV) of three replicates of each test were calculated Evaluation of assay specificity and sensitivity The diagnostic sensitivity (DSn) and specificity (DSp) of the ELISA...
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Báo cáo y học: "Validation of extravascular lung water measurement by single transpulmonary thermodilution: human autopsy study" doc
... correlation of those values in humans with a wide range of illnesses and injured lungs This correlation was also unaffected by sex, degree of LIS, pleural fluid amount, degree of CI, degree of CVP, ... correlation coefficients was tested by the z test after Gaussian transformation of the coefficients Reproducibility of EVLW measurements was assessed by the coefficient of variation (CV) and intraclass ... arterial partial pressure of oxygen/fraction of inspired oxygen Figure Correlation of extravascular lung water (EVLW) measured by single transpulmonary thermodilution and by postmortem lung weight...
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Development and validation of analytical method for naftopidil in human plasma by LC–MSMS
... chromatograms (a) chromatogram of blank plasma, (b) chromatogram of blank + IS, (c) chromtogram of LLOQ Development and validation of analytical method for Naftopidil in human plasma by LC–MS/MS lots were ... study of naftopidil in human plasma showed reliable stability behavior, as the mean of the results of the tested samples was within the acceptance criteria of ±15% of the initial values of the ... specificity of the method was investigated by comparing chromatograms obtained from six different sources of plasma The area observed at the retention time of naftopidil was much less than 20% of the...
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