uptake viability and proliferation of cells
Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot
... radioactive label of PC was similar in both control and HePC-treated cells and neither was the secretion of labelled PC altered by exposure to HePC (results not shown) Effect of HePC on choline uptake by ... amount of soluble and membrane-bound CT in the cell is to calculate the total activity of both forms for each dish (3 · 106 cells) Thus we determined the effect of 50 lM and 100 lM doses of HePC ... toxicity of HePC against HepG2 cells was also determined by the trypan blue exclusion assay and by measuring the release of lactate dehydrogenase (LDH) activity from the cytosol of damaged cells...
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báo cáo hóa học:" Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations" pptx
... effect of growth factors on proliferation of human NP cells (10%FBS/F12) (n = 20) Figure Dose-response effect of growth factors on proliferation of human NP cells (10%FBS/F12) (n = 20) Cells were ... degenerated disc cells, the structure and function of the degenerated cells changed remarkably Alini et al[11] observed an increase and maintain in density of bovine NP cells and synthesis Page of 11 (page ... of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Dose-response effect of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Cells were incubated with...
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Báo cáo khoa học: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells" doc
... change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells World Journal of Surgical Oncology 2010, 8:31 Page of ... significant Page of Results Proliferation Analysis SGC7901 cells were treated with different concentrations of arsenic trioxide (5 μmol/L, 10 μmol/L, 15 μmol/L and 20 μmol/L) at 24 and 48 hours The ... clumps of chromatin abutting to inner regions of the nuclear envelope between the nuclear pores, breakdown of nuclear envelope, chromatin condensation and dissociation of DNA fragments in SGC7901 cells...
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Báo cáo y học: " GDF-15 is abundantly expressed in plexiform lesions in patients with pulmonary arterial hypertension and affects proliferation and apoptosis of pulmonary endothelial cells" ppsx
... the cells of a plexiform lesion (P) Inset exhibits prominent luminal staining of GDF-15 in cells lining the vascular channel (arrow) Note the presence of GDF-15 in the endothelial cells of neigbouring ... http://respiratory-research.com/content/12/1/62 Page of 11 Effect of GDF-15 on sprouting of pulmonary endothelial cells To investigate the angiogenic effects of GDF-15 sprouting of human pulmonary microvascu-lar endothelial cells (HPMEC) ... endothelial cells To date little is known about the functional role of GDF-15 in endothelial cells A previous study demonstrated inhibitory effects of GDF-15 on proliferation, migration and invasion of...
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Báo cáo y học: "IL-13-induced proliferation of airway epithelial cells: mediation by intracellular growth factor mobilization and ADAM17" doc
... in defined apical and lateral regions of NHBE cells, could result in constitutive release of TGFα during exponential and stationary growth of these cells Constitutive release of TGFα is observed ... growth of control cells or on their constitutive release of TGFα (Figs 3b and 3c) Furthermore, while the presence of scrambled or ADAM17 antisense oligonucleotides reduced the maximal level of proliferation ... cellular proliferation mediated by TGFα in NHBE cells, we determined whether endogenous ADAM17 is involved in IL-13-induced proliferation of these cells First, the effects of various inhibitors of...
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Wheat germ agglutinin conjugated poly dl lactic co glycolic acid (PLGA) nanoparticles for enhanced uptake and retention of paclitaxel by colon cancer cells
... cytotoxicity profile of WGA against LS174T 158 5.4.4 Uptake of FITC-WGA 159 5.4.5 Antiproliferation activity of paclitaxel-loaded nanoparticles 160 5.4.6 Cellular uptake and efflux of paclitaxel ... the delivery of paclitaxel to colonic cancer cells Glycosylation patterns of representative colon cancer cells (Caco-2 and HT-29 cells) and normal cells (colon fibroblasts, CCD-18Co cells) were ... 3.3 Uptake of fWGA by (A) Caco-2, (B) HT-29 and (C) CCD-18Co cells as a function of incubation time (Data represent mean ± SD, n=4) 72 3.4 Uptake of fWGA by the Caco-2, HT-29 and CCD-18Co cells...
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ROLE OF THE ERP29 IN MIGRATION, APOPTOSIS AND PROLIFERATION IN BONE MARROW STEM CELLS
... induction of regulatory T cells (Treg cells) , steering monocytes and mature DC cells to an immature DC state, inhibition of B cell proliferation and terminal differentiation, and suppression of NK ... receptors and immunoglobulin production (Yi and Song, 2012) Figure 1.1 Diagram of Immunomodulatory Effect of BMSCs on Immune Cells Immunomodulatory effects of BMSCs include inhibition of T cell proliferation, ... 3.3.2 Knockdown of ERp29 decreased the expression of Pae3 and Par6 48 3.4 Knockdown of ERp29 increased apoptosis of BMSCs … 51 3.5 Knockdown of ERp29 decreased proliferation of BMSCs ……………...
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Báo cáo y học: " Effect of Weight Reduction on Cardiovascular Risk Factors and CD34-positive Cells in Circulatio"
... CD34+ cell number (given here as the ratio of the percentage of cells after the diet to the percentage of cells before the diet) and the percentage of body fat lost by each subject during the ... number of CD34-positive cells during treatment may provide an indicator of improvement of vascular health In our study, we analyzed the effect of weight loss on the improvement of lipid profile ... International Society of Haematotherapy and Graft Engineering (ISHAGE) guidelines [27] The method of the selection of stem/progenitor cells consisted from several criteria Cells were selected that...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... diameter of the PVDF membrane, the PVDF-PS membrane and the PVDF-SPS membrane is 0.25 µm, 0.12 µm and 0.16 µm, respectively It can be seen that the polymerization of styrene and the blending of PS and ... polystyrene polymer and the PVDF matrix 3.5 Water uptake of the PVDF-SPS composite membrane Table shows the water uptake of the pristine PVDF membrane, the PVDF-PS membrane and the PVDF-SPS composite ... blending of PS polymer reduces the water uptake of the PVDF membrane decreasing from 22.05% to 14.31%, which can explain the hydrophobic property of PS polymer However, the water uptake of PVDF-SPS...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
... Characterization of the binding of PCSK9 to intact cells S A Mousavi et al Fig Dissociation time courses and effects of unlabeled ligands and low pH on the dissociation rates of [125I]TC-PCSK9 (A) and [125I]TC-PCSK9-D374Y ... Characterization of the binding of PCSK9 to intact cells Fig Nonlinear regression and Scatchard analyses of binding-inhibition data (A) Inhibition of [125I]TC-PCSK9 (2.5 lgÆmL)1) binding to HepG2 cells ... kinetics of binding of PCSK9 to HepG2 cells can be described as a simple bimolecular reaction, the kinetics of [125I]TC-PCSK9 and [125I]TC-PCSK9-D374Y dissociation from and association with HepG2 cells...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... effects of MSCs in vitro mediator of the cells paracrine anti-fibrotic effects These findings help to improve our understanding of the cellular and molecular basis of MSCs’ anti-inflammatory and paracrine ... presence and absence of IL-10 *P < 0.05 and **P < 0.01 versus Cont group (D) Representative western blots for collagen I and III in the presence and absence of 0.1 lM angiotensin II and IL-10 ... fibroblast proliferation and collagen expression The molecular mass of IL-10 is 19 kDa, which is < 30 kDa and thus part of the MSCs-CM fraction that inhibited cardiac fibroblast proliferation and collagen...
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Tài liệu Báo cáo khoa học: Efficient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf
... after 16, 24 and 48 h of culture using trypan blue staining (C) Cells were treated with 0.5, and lg of hpST3dODN and and lg of control ODN for h or with lipids only; after 48 h of culture, they ... with IFN-c induces cell death of SW480 cells (A) SW480 cells were incubated in the absence of IFN-c or with 10, 100 and 200 ngÆmL)1 for 4, 24 and 48 h of incubation and cell death was measured ... h, and became detectable after 16, 24 and 48 h (Fig 1B); after 72 h, the amount of dead cells and debris made it difficult to count dead cells Analysis by flow cytometry clearly showed the cells...
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Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx
... shows that treatment of the cells with BEL induced a significant accumulation of cells in G2 ⁄ M and S, and a concomitant decrease of cells in G1, with respect to untreated cells In contrast, pyrrophenone ... [BEL] (µM) B B Number of cells × 105 Number of cells × 105 12 10 0 12 24 10 [MAFP] (µM) 36 48 12 10 Time (h) 12 24 36 48 Time (h) Fig Effect of BEL on the growth of U937 cells (A) Dose– response ... lactone (BEL) [11,12] In INS-1 insulinoma cells, acute inhibition of iPLA2-VIA reduces the relatively high content of lysoPC of these cells by about 25% [12], and the decrease is about 50–60% in macrophage-like...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... concentration of G418 (Invitrogen) of 400 lgÆmL)1 G418-resistant colonies were picked and expanded for PCR genotyping and the formation of embryoid bodies One ESC clone (T26) out of 48 G418-resistant ... buffer and applied onto MACS columns Magnetically selected positive cells and depleted cells were cultured independently as lymphatic endothelial cells and blood vascular endothelial cells, respectively ... specialized techniques and expertise that are required for immunological isolation of ECs [23,24] to prevent proliferation of tsA58T Ag-expressing non-ECs Results and Discussion Generation of a transgenic...
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Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc
... FACS analysis; cells were gated as GFP-positive based on fluorescence of uninfected cells, and the percentage of GFP positive cells is shown Error bars represent the standard deviation of triplicate ... detectable homology to CD40 ligand (data not shown) and that enhances adenoviral infection of CD40-positive cells of both human and murine origin, including DC and B cells resistant to infection ... (filled) cells The gate shown represents GFP-positive cells as determined by fluorescence of uninfected cells Some 43.7% of CD40 transfected cells were determined to be GFP positive vs 12.6% of mock-transfected...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... expression of HIV- and FLAG-tagged D2L and D2S receptors in Sf9 cells To achieve this, mAbs directed against gp120 (clone 11/4C) and the FLAG sequence were used, and Fig shows the band pattern ... Sf9 cells and HEK293 cells, the three ligands tested had no effect on the FRET signal observed, as shown in Fig 6B,C Discussion Lack of regulation of D2 receptor oligomerization by the ligands ... D2L and D2S dopamine receptors expressed in Sf9 cells and D2S receptor expressed in HEK293 cells Both immunological and fluorescence-based approaches provide evidence that the two isoforms of D2...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... Sertoli cells by single step method of Chomczynski & Sacchi [29] using Trizol reagent The integrity and quality of puri®ed RNA were controlled by 1% agarose gel electrophoresis and measure of the ... (control) of FSH (100 ngáml)1) and of bFGF increasing concentrations Determination of estradiol production was performed by radioimmunoassay Values are expressed in pg per 106 cells and are representative ... of Ultroser before being used on day after plating For the aromatase assay, Sertoli cells were incubated for 24 h with testosterone (200 ngámL)1), oFSH (100 ngámL)1) and/ or bFGF (5 ngámL)1) and/ or...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... some of which (e.g modulation of cytokine release from macrophages and inhibition of lymphocyte proliferation) resemble those of THC, while some others (e.g stimulation of hematopoietic cell proliferation) ... Purification and quantification of endocannabinoids The extraction, purification and quantification of anandamide, 2-AG and PalEtn from immature and mature dendritic cells requires a set of different biochemical ... 107 cells for AEA, PalEtn and 2AG, respectively Analysis of cannabinoid receptors and fatty acid amide hydrolase To determine the presence of the cannabinoid receptors (CB1 and CB2) and of the...
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Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system doc
... al [3] In a series of publications, it was shown that a single application of ultrasound could result in a dramatic loss of germ cells from testes and that this loss of germ cells was reversible ... count ranges and the percentage of counts that fell within each range For example, if 75% of a group’s sperm counts fell in the second range of 11-20 × 10 and the remaining 25% of the counts ... power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system Reproductive Biology and Endocrinology...
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Báo cáo khoa học: MicroRNA-143 reduces viability and increases sensitivity to 5-fluorouracil in HCT116 human colorectal cancer cells potx
... miR-143 and 5-FU down-regulate ERK5, NF-jB and Bcl-2 protein expression Populations of HCT116 cells, miR-143 overexpressing cells (HCT116-OV3 and pCR3-pri-miR-143 mix) and respective control cells ... HCT116 cell viability Cells were transfected with the indicated plasmids and 50 or 100 nM anti-miR inhibitors, and analyzed at 48 h post-transfection (A) Cells were lysed and firefly and renilla ... HCT116-OV3 and HCT116-EM1 cells (A, B) Cells with higher miR-143 expression (HCT116-OV3 and pCR3-pri-miR-143 mix) displayed decreased viability and increased cell death after exposure to 5-FU Cell viability...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25