trypsin serine protease inhibition activity
Báo cáo khoa học: Human airway trypsin-like protease induces amphiregulin release through a mechanism involving protease-activated receptor-2-mediated ERK activation and TNF a-converting enzyme activity in airway epithelial cells doc
... of the cDNA for human airway trypsin- like protease J Biol Chem 273, 11895–11901 Hooper JD, Clements JA, Quigley JP & Antalis TM (2001) Type II transmembrane serine proteases-insights into an emerging ... human airway trypsin- like protease on 6398 13 14 15 16 17 intracellular free Ca2+ concentration in human bronchial epithelial cells J Med Invest 50, 95–107 Schmidlin F & Bunnett NW (2001) Protease- activated ... pseudopodia during protease- activated receptor-2-induced chemotaxis J Biol Chem 278, 34418–34426 Darmoul D, Gratio V, Devaud H & Laburthe M (2004) Protease- activated receptor in colon cancer Trypsininduced...
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Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf
... other serine proteases are probably involved in the activation of HGF in these tissues Several serine proteases have been shown to convert pro-HGF to the active form in vitro They include serine proteases ... assay of inhibition by HAI-1 Protease TMPRSS13 is inhibited by HAI-1 A Hepatocyte growth factor activator inhibitor type-1 (HAI-1) (1) N C N Inhibition of TMPRSS13 protease activity ... and protease activity was measured using synthetic substrates suitable for each TTSP TMPRSS3 and TMPRSS4 expressed in this system did not show protease activity Thus, other TTSPs that did show activity...
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Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx
... gene promoter activity, and that this suppressive effect of NS3-4A was dependent on its serine protease activity, because the NS3-4A ⁄ S1165A mutant lacking the serine protease activity did not ... These cleavages are mediated by the host and virally encoded serine protease located in the amino-terminal domain of NS3 Serine protease activity of NS3 requires NS4A, a protein consisting of 54 ... encoding the NS3-4A ⁄ S1165A mutant (1B-1 strain) lacking serine protease activity or the NS3-4A ⁄ W1528A mutant (1B-1 strain) lacking RNA helicase activity was also used for the transfection The lysate...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
... similar purity ATP-stimulated protease activity in aging rats Fig Lon protease quantification and activity in liver mitochondrial matrix from 10- and 27-month-old rats Activity was determined in ... homogenate) The activity of ATP-stimulated protease was determined in the presence or absence of ATP (Fig 2A) Protease activity in the absence of ATP was lower in aging rats than in adults Activity ... integrity Lon protease plays a pivotal role because it is responsible for breaking down abnormal matrix proteins In our study, we have shown a large decline in ATPstimulated Lon protease activity...
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Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf
... several serine proteases not have an aspartate as the catalytic apparatus M NaCl However, for chymotrypsin-type serine proteases, the replacement of this aspartate with an alanine diminishes protease ... Achromobacter lyticus protease I, a lysine-specific serine protease J Biol Chem 264, 3832–3839 Perona, J.J & Craik, C.S (1995) Structural basis of substrate specificity in the serine proteases Protein ... the sidechain of Lys (API) or Lys and Arg (trypsin) The aromatic stacking between Trp169 and His210 in API is unique among chymotrypsintype serine proteases MATERIALS AND METHODS Materials The...
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Báo cáo khoa học: CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site doc
... activates the complement system, a function that is dependent on its associated serine proteases, the MBL-associated serine proteases (MASPs), which initiate the lectin complement cascade by cleavage ... (2000) Cutting edge: complement-activating complex of ficolin and mannosebinding lectin-associated serine protease J Immunol 164, 2281–2284 Matsushita M, Kuraya M, Hamasaki N, Tsujimura M, Shiraki H ... (MBL) co-receptor calreticulin interacts with MBL through the binding site for MBL-associated serine proteases FEBS J 275, 515–526 Fass D, Blacklow S, Kim PS & Berger JM (1997) Molecular basis...
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TẠO DÒNG VÀ PHÂN TÍCH TRÌNH TỰ GEN SERINE PROTEASE THỦY PHÂN FIBRIN TỪ GIUN QUẾ (Perionyx excavatus) pot
... M and Nakajima, Molecular cloning, sequencing, and expression of cDNA encoding serine protease with fibrinolytic activity from earthworm, Biosci Biotechnol Biochem., 65 (7), (2001), 1575-1580 ... Hình Điện di sản phẩm PCR M: Marker; 1, 2: Sản phẩm PCR 32 Tạo dòng phân tích trình tự gen serine protease Hình Điện di plasmid sau cắt enzyme cắt hạn chế EcoRI M: marker; 1: plasmid tái tổ ... AAATCAAACA 120 130 140 150 160 170 180 | | | | | | | | | | | | Tạo dòng phân tích trình tự gen serine protease 34 121 ATTTTAGCAC ACCTCTTAGG CACCATTTAA TAAGAAAAAT CAATATTAAT TTCCCAAAAA 180 190 200...
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Báo cáo khoa học: Hepatocyte growth factor activator (HGFA): a serine protease that links tissue injury to activation of hepatocyte growth factor pdf
... of the catalytic domain of serine proteases (Fig 1A) [5] HGF, however, has no proteolytic activity, because two of the conserved catalytic triad residues of the serine protease domain are substituted ... through a region corresponding to the ‘active site’ and ‘activation domain’ of serine proteases [9,10] HGF and serine proteases thus share a similar activation mechanism Proteolytic activation of ... HGF activity during mammalian development Known proteases that activate HGF in vitro not appear to be the key proteases activating HGF during embryonic development, because mice lacking these proteases...
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Báo cáo khoa học: Effects on protease inhibition by modifying of helicase residues in hepatitis C virus nonstructural protein 3 pot
... level Inhibition of NS3 protease variants The effect of changes in the helicase residues on the inhibition of the protease was investigated for the three variants and a series of inhibitors Inhibition ... with other functional properties, and have analysed how this affects protease activity and inhibition For the analysis of modied inhibition characteristics, a set of 11 inhibitors with varying structure, ... full-length HCV NS3 can inuence the inhibition of the protease As a consequence, anti-HCV protease drug discovery using full-length HCV NS3 as a model system for inhibition studies may be more appropriate...
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Báo cáo khoa học: A novel serine protease highly expressed in the pancreas is expressed in various kinds of cancer cells potx
... A novel serine protease in various cancer cells serine protease, spinesin ⁄ TMPRSS5, which is localized at synapses [6] Motopsin (PRSS12) is a mosaic serine protease, with a kringle ... 2005 FEBS A novel serine protease in various cancer cells 4913 A novel serine protease in various cancer cells identity, respectively (Fig 2) Amino acid alignment of these proteases shows that ... a cDNA of 958 bp that encodes a novel serine protease of 317 amino acids from human brain cDNA pool The predicted amino acid sequence includes a serine protease motif, and this protein was designated...
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Báo cáo khoa học: Spermosin, a trypsin-like protease from ascidian sperm cDNA cloning, protein structures and functional analysis doc
... previously reported that ascidian spermosin is a novel sperm trypsin- like protease, distinct from acrosin, a well-known sperm trypsin- like protease that is widely distributed in mammalian sperm, in ... and heavy chains is rather common in serine proteases including mouse testis-speci®c proteases (TESP-I and -II) [20], ascidian acrosin [16], and mammalian proteases such as thrombin, kallikrein, ... spermosin heavy chain with those of trypsinfamily proteases including ascidian acrosin by the CLUSTALW program Identical residues in the sequences among serine protease are indicated by asterisks...
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Báo cáo khoa học: NtKTI1, a Kunitz trypsin inhibitor with antifungal activity from Nicotiana tabacum, plays an important role in tobacco’s defense response pot
... fungal inhibition around the disks were checked daily The plates produced crescents of inhibition around disks containing samples with antifungal activity The assay for antibacterial activity ... pathogen-induced protease (PR-P69) in tomato plants: similarity of functional domains to subtilisin-like endoproteases Proc Natl Acad Sci USA 93, 6332–6337 Antao CM & Malcata FX (2005) Plant serine proteases: ... indicated by an arrow in the right lane (B) In vitro bovine trypsin inhibition by the recombinant NtKTI1 protein (open circles) Soybean trypsin inhibitor (triangles) was assayed in parallel as a...
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QUY TRÌNH TẠO DÒNG GEN SERINE PROTEASE THỦY PHÂN FIBRIN Ở ESCHERICHIA COLI SỬ DỤNG BỘ KIT TA CLONING
... TẠO DÒNG GEN SERINE PROTEASE TỪ DNA GIUN ĐỎ Quy trình chung Thu lượng lớn đoạn DNA Lumbricus rubellus mã hóa cho enzyme lumbrokinase (serine protease gen) Bước 1: Chuẩn bị DNA serine protease gen: ... dòng serine protease gen Bước 5: Kiểm tra tế bào chọn cách dùng enzyme cắt giới hạn, phân tích trình tự Figure 1: Quy trình chuẩn bị DNA vector cho biến nạp sử dụng T-Vector Quy trình tạo dòng serine ... M and Nakajima, Molecular cloning, sequencing, and expression of cDNA encoding serine protease with fibrinolytic activity from earthworm, Biosci Biotechnol Biochem., 65 (7), (2001), 1575-1580...
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Báo cáo sinh học: " Neutrophil elastase, an acid-independent serine protease, facilitates reovirus uncoating and infection in U937 promonocyte cells" pdf
... require cysteine protease activity and is not blocked in the presence of agents that raise vesicular pH Studies using protease inhibitors suggest that, in the absence of cysteine protease activity, ... under which lysosomal cysteine protease activity was inhibited Cells were treated with 300 µM E64, a broad-spectrum cysteine protease inhibitor [46], and protease activity was assessed using the ... aciddependent proteases Cat L and Cat B mediate uncoating [21,23] Serine proteases, including NE, and metalloproteases function over a broader pH range Therefore, to gain insight into the nature of the protease( s)...
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báo cáo hóa học:" Neutrophil elastase, an acid-independent serine protease, facilitates reovirus uncoating and infection in U937 promonocyte cells" pptx
... require cysteine protease activity and is not blocked in the presence of agents that raise vesicular pH Studies using protease inhibitors suggest that, in the absence of cysteine protease activity, ... under which lysosomal cysteine protease activity was inhibited Cells were treated with 300 µM E64, a broad-spectrum cysteine protease inhibitor [46], and protease activity was assessed using the ... aciddependent proteases Cat L and Cat B mediate uncoating [21,23] Serine proteases, including NE, and metalloproteases function over a broader pH range Therefore, to gain insight into the nature of the protease( s)...
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Báo cáo hóa học: " Identification and characterization of alkaline serine protease from goat skin surface metagenome" docx
... protease activity Even at mM concentration, Zn2+, Hg2+ and Ni 2+ did not affect the protease activity whereas Fe 2+ significantly inhibited protease activity However, Co2+ and Mn2+ enhanced protease ... purified AS -protease was examined at 37°C Purified AS -protease exhibited maximum activity at pH 10.5 (Figure 6A), confirming it to be an alkaline protease This protease exhibited 75 85% of activity ... Bacillus stearothermophilus protease promoter Protease promoter represents the predicted alkaline serine protease promoter region E coli protease promoter represents, E.coli lon protease promoter Effect...
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Structural and functional characterization of TRX16, a thioredoxinlike protein and altering substrate specificity of a serine protease inhibitor
... developed unique modalities with serine protease inhibitors to detect and respond to microbial and host proteases Two isoforms of immunomodulatory two-domain Kazal-like serine protease inhibitors, CrSPI-1 ... 84 4.2.6 Stability verification of CrSPI-1-D1 mutants against serine proteases 85 4.2.7 Inhibition of Thrombin Amidolytic Activity 85 4.2.8 Isothermal Titration Calorimetry (ITC) ... loop regions from P3 to P4’ position of selected serine protease inhibitors 95 Table 4.4: IC50 and dissociation constant (Kd) for the inhibition of thrombin by various variants of...
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Structural studies of cysteine and serine protease inhibitors towards therapeutic applications
... 1.4.1Catalytic mechanism of Serine proteases 23 1.4.2 Serine protease inhibitors 26 1.4.3 Serpins 27 1.4.4 Canonical serine protease inhibitors 29 1.4.5 Non canonical serine protease inhibitors 31 ... carried out by serine proteases Trypsin and Chymotrypsin An inherited autosomal recessive disorder cystic fibrosis is caused by the deficiency in the transport of Trypsin, a serine protease secreted ... related to serine and cysteine proteases and their inhibitors Serine proteases and Cysteine proteases have similar catalytic triad residues apart from the nucleophilic residue of cysteine or serine...
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Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc
... other serine (trypsin and chymotrypsin) and one aspartic (pepsin) proteases The results showed that this extension is also not cleaved by any of these proteases in their optimum condition of activity ... proteases Erv-A, -B, -C and papain from the plant latex; two serine proteases trypsin and chymotrypsin from bovine pancreas; one aspartic protease pepsin from porcine stomach mucosa), each having ... papaya (Merck, Kenilworth, NJ, USA)], two serine proteases [trypsin and chymotrypsin from bovine pancreas (Sigma-Aldrich, St Louis, MO, USA)], one aspartic protease [pepsin from porcine stomach mucosa...
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