totipotency pluripotency and embryonic stem cells

báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and ... AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... as: Rada-Iglesias A, Wysocka J: Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease Genome Medicine 2011, 3:36...

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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28

Danh mục: Thạc sĩ - Cao học

... Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15 ... Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem cells ... family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass...

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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Danh mục: Báo cáo khoa học

... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time points ... structure modiﬁcations and factor recruitment at the murine b-globin locus in uninduced embryonic stem cells (day 0), as well as that of primitive and deﬁnitive erythroid cells (days and 12, respectively)...

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embryonic stem cells, methods and protocols - kursad turksen

Danh mục: Sinh học

... Mouse Embryonic Stem Cells Pierre Savatier, Hộlốne Lapillonne, Ludmila Jirmanova, Luigi Vitelli, and Jacques Samarut 27 Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis ... Stem Cells 1 Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells Marsha L Roach and John D McNeish Introduction Embryonic stem (ES) cells were rst isolated in the 1980s by ... 107 12 Embryonic Stem Cell Differentiation and the Vascular Lineage Victoria L Bautch 117 13 Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth...

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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Danh mục: Hóa học - Dầu khí

... Human embryonic stem (hES) cells possess unique features: self-renewal and pluripotency They can be continuously cultured in undifferentiated status and give rise to differentiated cells and tissues ... either hES cells or adult cells (Figure 6, panel B) For miR-106b, miR-92, miR-93, miR-130a and miR-190, the difference in their expression between EB and hES cells and between EB and adult cells were ... and miR-520b were much higher in hES cells than in either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and...

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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES doc

Danh mục: Sức khỏe giới tính

... stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... tremendous variety of approaches and techniques, and this book, Embryonic Stem Cells - Differentiation and Pluripotent Alternatives and its companion, Embryonic Stem Cells - Basic Biology to Bioengineering, ... pathways in human embryonic stem cells Stem Cells, 2006 24(6): p 1476-86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonic stem cells Int J Mol Med,...

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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES pdf

Danh mục: Sức khỏe giới tính

... stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... tremendous variety of approaches and techniques, and this book, Embryonic Stem Cells - Differentiation and Pluripotent Alternatives and its companion, Embryonic Stem Cells - Basic Biology to Bioengineering, ... pathways in human embryonic stem cells Stem Cells, 2006 24(6): p 1476-86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonic stem cells Int J Mol Med,...

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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf

Danh mục: Báo cáo khoa học

... adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells may ... and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells Trends Biotechnol 2004, ... transplantation, embryonic stem cells, and the potential for cell therapy N Eng J Med 2003, 349, 275-286 27 Hogan B, Beddington R, Costantini F, Lacy E Isolation, culture and manipulation of embryonic stem cells...

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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt

Danh mục: Báo cáo khoa học

... and in vivo mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10:1518-1525 17 Chaudhry GR, Yao D, Smith A, Hussain A: Osteogenic Cells Derived From Embryonic ... Luo K, Link C: Embryonic stem cells as an alternate marrow donor source: engraftment without graft-versushost disease J Exp Med 2004, 199:895-904 36 Zavazava N: Embryonic stem cells and potency ... neural precursors from human embryonic stem cells Nat Biotechnol 2001, 19:1129-1133 doi:10.1186/1746-160X-7-12 Cite this article as: Handschel et al.: Embryonic stem cells in scaffoldfree three-dimensional...

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Danh mục: Báo cáo khoa học

... phosphatase and Oct-4 to verify pluripotency of the ESC lines were in agreement (χ2 = 0.105), while TROMA-1 identified endoderm and trophoblast Pluripotency was successfully confirmed in at least some cells ... Biology and Medical Modelling 2005, 2:25 http://www.tbiomed.com/content/2/1/25 Figure Experimental embryonic stem cell colonies derived from a single blastomere Experimental embryonic stem cell ... JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J Clin Invest...

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Báo cáo y học: "Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity" pps

Danh mục: Báo cáo khoa học

... [1,2] Other adult stem cells include neural stem cells (NSCs) [3], and the mesenchymal stem cells (MSCs) that give rise to osteoblasts, chondrocytes, adipocytes, and skeletal and smooth muscle myocytes ... neural stem cells Science 2000, 287:1433-1438 Prockop D: Marrow stromal cells as stem cells for nonhematopoietic tissues Science 1997, 276:71-74 Smith AG: Embryo-derived stem cells: of mice and ... developmental path to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/ progenitor cells Stem Cells 2006, 24:889-895 NIA array analysis [http://lgsun.grc.nia.nih.gov/ANOVA/...

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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration

Danh mục: Cao đẳng - Đại học

... relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee ... engineering, and the challenges that need to be overcome before clinical application which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem ... regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonic stem cells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells for their self-renewal and propagation...

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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives

Danh mục: Cao đẳng - Đại học

... underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics ... variety of cells that make up the central nervous system including dopaminergic neurons, motor neurons, glial cells, astrocytes, oligodendrocytes, neural crest stem cell cells and retinal cells (Bjorklund ... 9! 2.4! SHH and neural development 10! iii 2.5! SHH and proliferation 14! 2.6! SHH in developmental disorders and cancer 15! 2.7! Embryonic stem cells and induced...

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Identification of oct4 and sox2 targets in mouse embryonic stem cells

Danh mục: Cao đẳng - Đại học

... (2007) 1.2 Embryonic stem cells (ESCs) Cells from the ICM can be isolated and cultured in vitro under permissive conditions as embryonic stem cell (ESC) lines (Bongso et al., 1994; Evans and Kaufman, ... Multipotent stem cells, with restricted differentiation potential, can be found in the umbilical cord and adult tissues such as haematopoietic stem cells that give rise to blood cells (Moore and Lemischka, ... work xix CHAPTER GENERAL INTRODUCTION 1.1 Stem cells Stem cells are classically defined as cells that can generate daughter cells identical to their parent cells (self-renewal) as well as can produce...

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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders

Danh mục: Cao đẳng - Đại học

... somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have ... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stem cells ... types and tissues including fat cells, cartilage, bone, tendons and ligaments, muscle cells, skin cells and even nerve cells (Short et al 2003) Neuronal stem cells The discovery of neuronal stem cells...

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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages

Danh mục: Tổng hợp

... 1.1 Embryonic stem cells 1.2 Gastrulation– formation of mesoderm and endoderm in the embryo .8 1.3 Regenerative medicine and embryonic stem cells 10 1.3.1 Diabetes– a candidate ... Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure 1.3 Gastrulation and specification ... μ – Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising...

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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery

Danh mục: Kỹ thuật - Công nghệ

... literature review and comparison for all three types of stem cells, adult stem cells, embryonic stem cells and induced pluripotent stem cells, embryonic stem cells are inevitable and irreplaceable ... Biocompatibility 1.2 Stem cells ………………………………………………………………….4 1.2.1 Significance in the use of stem cells 1.2.2 Definition of stem cells 1.2.2.1 Adult stem cells 1.2.2.2 Embryonic stem cells 1.2.2.3 Induced ... the use of adult stem cells either in research or clinical applications 1.2.2.2 Embryonic stem cells: Another type of stem cells by conventional categorization is embryonic stem cells based on...

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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells

Danh mục: Tổng hợp

... humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse Embryonic Stem Cells ... negative control cells Mouse embryonic stem cells transfected with pcDNA6/TR and surviving Blasticidin selection were verified for proper expression of the Tet-R protein via RT-PCR and Western blot ... short, hairpin RNA system Introduction Mouse embryonic stem cells (mESC) are uniquely capable of differentiating into all somatic cell types in the body, a property known as pluripotency In addition,...

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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"

Danh mục: Y học thưởng thức

... Aqua, and DAPI Images were captured, processed, and analyzed using ISIS mBAND/mFISH imaging software (MetaSystems GmbH, Altlussheim, Germany) In the mFISH technique, each chromosome (1–22 and X and ... M, Fong CY, Tan S, Chan WK, Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, ... B and C colonies were 94.5%, 3.0% and 2.5% respectively for 24h exposure to 4oC (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The proportion of Grade A, B and...

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Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx

Danh mục: Báo cáo khoa học

... ﬂox cells, we failed to establish Ptb) ⁄ ) ES cells Then, we expressed Cre by retrovirus infection into Ptb) ⁄ ﬂox cells (Fig 1C) To identify Ptb) ⁄ ) ES cells, we screened those cells by PCR and ... blue-staining-negative cells to that of total cells Cells (3 · 105) were seeded on growth medium and counted every day for days of culture The circles indicate the values for wild-type cells and the triangles ... the expression of differentiated markers in wild-type and Ptb) ⁄ ) ES cells and EBs from wild-type and Ptb) ⁄ ) ES cells () ⁄ )1) Fgf5, Gata4 and Gata6 transcripts were normalized to Gapdh transcripts...

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