time amp hyphen lapse imaging of mhci internalization in gfp rab22a expressing cells
báo cáo khoa học: "Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy" pps
... required staining Figure shows that even without staining this imaging method can readily explore in detail the dissimilarities in the internalization of different AuNPs by different cell lines Specifically, ... EMT cells after co-culturing with 500 μM naked AuNPs for 48 h The size distribution in A) was obtained by analyzing individual cells such as that in B) Bars in B: 10 μm Chen et al Journal of ... Figure 8C and 8D (movie of different projection in Additional file 2) shows the results of specimen staining in revealing the detailed localization and shape of the nucleus and of the overall nanoparticle...
- 15
- 351
- 0
báo cáo khoa học: " Computed tomography imaging of subpleural lipoma in two men: two case reports" potx
... entities since the only key finding might be the mitosis rate of the tumor cells [2] However, CT is able to depict potential invasion of adjacent structures such as the ribs or the soft tissue of the ... the editing of the manuscript OFD was responsible for creating the figure files SL contributed to the editing and writing of the manuscript All authors read and approved the final version of the ... attacks of bronchitis Discussion Before the implementation of computed tomography in diagnostic medical imaging, most subpleural lipomas were detected during autopsies or incidentally during thoracic...
- 4
- 279
- 0
Báo cáo thú y: "Survey radiography and computerized tomography imaging of the thorax in female dogs with mammary tumors" pot
... lymphatic drainage in the other mammary chain Four female dogs (Cases 1, 3, and 21) had already been spayed Before the imaging examinations in all female dogs, fine-needle aspiration cytology of the ... The dogs were maintained in spontaneous breathing during CT examination due to the type of anesthesia used However, the images obtained during the apneic period and at the inspiration peak are ... TW 2007, 729-735 24 25 26 Sorenmo KU, Shofer FS, Goldschmidt MH: Effect of spaying and timing of spaying on survival of dogs with mammary carcinoma J Vet Intern Med 2000, 14:266-270 Chang SC, Chang...
- 10
- 434
- 0
Báo cáo khoa học: "Quantifying bedside-derived imaging of microcirculatory abnormalities in septic patients: a prospective validation study" docx
... assessment of interrater variability, each of two blinded investigators scored the flow in each sample independently For the sublingual region there were 224 samples available In 202 (90%) samples ... part of treatment of an abdominal sepsis OPS imaging was performed both in the sublingual region and in a stoma during the intensive care unit stay on days 1, and after the surgical procedure In ... villi of the small intestine Orthogonal polarization imaging of a repeating vascular structure; the villi of the small intestine Objective 5×, on screen 325× method has yet been validated in terms...
- 6
- 180
- 0
Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma" ppsx
... bioluminescence imaging of intact cells HSV-TK-Luc expressing U87 glioma cells were transferred to a black microtiter plate in order to minimize light scattering, and MTT assay was performed in ... bioluminescence imaging and measurement of tumour size by caliper HSV-TK-Luc expressing U87MG glioma cells were also detected by the CCD camera after inoculation of × 106 cells intracerebrally in ... clones expressing unfused HSV-TK or Luc Cells stably expressing HSV-TK did not luminesce upon addition of D-Luciferin while Luc expressing cell clones were resistant to GCV mediated cell killing...
- 13
- 388
- 0
báo cáo hóa học: " Dexamethasone diminishes the pro-inflammatory and cytotoxic effects of amyloid β-protein in cerebrovascular smooth muscle cells" docx
... anti-inflammatory drugs in the treatment of AD success in slowing disease progression has not been forthcoming in clinical trials [44] This lack of success may reflect intervention too late in ... HCSM cells were rinsed with phosphate-buffered saline, stained with 0.1% thioflavin T for 10 at room temperature and rinsed with 80% ethanol three times Then 250 μl of phosphate-buffered saline ... striking accumulation of cellassociated AβPP [33,34] This consequence is mediated through its high-affinity binding to the cell surface Aβ fibrils via a domain in the amino terminal region of AβPP...
- 8
- 389
- 0
Báo cáo sinh học: "Compound developmental eye disorders following inactivation of TGF signaling in neural-crest stem cells" doc
... cultured in DMEM:F12 medium (Gibco/Invitrogen, Carlsbad, USA) containing 10% fetal bovine serum (Sigma) Following a 60 incubation in DMEM:F12 medium containing 0.1% bovine serum albumin at 37°C, cells ... anomalies in Tgfbr2-mutant mice (a) Toluidine blue staining of semi-thin sagittal sections of eyes at E18 reveals a smaller size with no anterior chamber and an infiltration of cells behind the lens in ... distinct stages of development [22] At E15, the inner parts of the retina from control mice strongly expressed the transcription factors Brn3A in retinal ganglion cells and Pax6 in amacrine cells...
- 16
- 303
- 0
PKCα mediated induction of miR-101 in human hepatoma HepG2 cells docx
... conditions PKC is a member of serine/threonine kinase whose isoforms have been shown to be involved in a number of cellular processes, including cell proliferation, apoptosis, invasion and migration ... tissues and induced over 4-fold in HepG2 cells upon TPA treatment (Fig 2B; Table 2) The induction kinetics of both miR-101 and miR-29c in HepG2 cells after TPA treatment were examined Interestingly, ... HepG2 cells were seeded in 24-well plates overnight in DMEM containing 10% (v/v) FBS Before colorimetric determination of MTT reduction, MTT was added to a final concentration of 0.5 mg/ml, and incubation...
- 9
- 168
- 0
báo cáo khoa học: " Microarray-based analysis of microRNA expression in breast cancer stem cells" doc
... staining and immunohistochemical staining The tumors in the BCSCs group were positive for both EMA and SMA, indicating that they were included of both luminal epithelial and myoepithelial cells ... other stem cells, indicating that these miRNAs may play a constitutive role in maintaining the biological characteristics of stem cells [40,41] Future work should include verification of the potential ... expressed in MCF-7 cells, may be one of the causes of high expression of the drug-metabolising enzyme CYP1B1 in cancerous tissues [14] Finally, as a tumor suppressor in breast cancer cells, miR-17-5p...
- 8
- 333
- 0
Báo cáo y học: " Peptides derived from the HIV-1 integrase promote HIV-1 infection and multi-integration of viral cDNA in LEDGF/p75-knockdown cells" doc
... resulting in inhibition of IN nuclear import [18,21] as well as of its enzymatic activity [17,22,23] Rev-induced inhibition of the IN enzymatic activity resulted in inhibition of cDNA integration ... therefore of interest to determine whether the INS peptide, in addition to its binding to IN and Rev, is also able to interact with the LEDGF/p75 protein ELISA binding studies revealed specific binding ... absence of the LEDGF/p75 protein in these cells, all of the IN molecules are available for interaction with Rev, resulting in the formation of inactive Rev -IN complex and complete inhibition of cDNA...
- 11
- 284
- 0
Báo cáo y học: "Atorvastatin reduces lipopolysaccharide-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells" ppsx
... protein atorvastatin on LPS-induced expression of COX-2 Effect of3 Effect of atorvastatin on LPS-induced expression of COX-2 protein A549 cells were treated as described in Figure After incubation, ... effects of atorvastatin COX-2 protein Time- dependentPGE2 mRNA (red line),on LPS-induced Time- dependent effects of atorvastatin on LPS-induced expression of COX-2 mRNA (red line), COX-2 protein (green ... reduction of the production of pro-inflammatory cytokines Statins inhibit the Ang II-induced secretion of interleukin-6 (IL-6) in cultured human vascular smooth muscle cells, and decrease production of...
- 6
- 268
- 0
Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot
... fibroblasts remained after 20-25 minutes plating in non-gelatinized plates Pre-plating of feeder-dependent ES cells in this way may result in a slight delay in the apparent time of replication ... replication in ES cells is altered by mutation of chromatin modifiers Satellite Satellite replication in ES cells is altered by mutation of chromatin modifiers (a) Summary of replication timing of repeat ... Poole, UK) of the D3 clone Deletion of both alleles was confirmed by genotyping The ES cell lines were maintained in the undifferentiated state by culturing on gelatinized plates in KO-DMEM (Invitrogen,...
- 13
- 373
- 0
Experimentation, modeling and control of calcium dynamics in human vascular endothelial cells
... and engineering perspectives Proficient skills in handling cells and chip In our case, we need to integrate cell culture, ATP release measurement and Ca2+ imaging into the one system The main reason ... 1.1.1 Views of Biologists Endothelium is a monolayer of cells lining the inner wall of blood vessel and works as a barrier separating the blood flow and vascular muscle cells As continuously exposed ... “uncomfortable” to engineers who have been long working with machines and tend to step into the world of mechanobiology in the very beginning In the subsection below, another version of statement is...
- 96
- 259
- 0
Báo cáo y học: "Stochastic modeling of oligodendrocyte generation in cell culture: model validation with time-lapse data" pdf
... assumption by fitting the model to experimental data on the evolution (over time) of clones consisting of two distinct types of cells However, if one considers the whole population of cells, there ... pl = min{p + qrl, 1}, where p, q and r are free positive parameters with p representing the limiting probability of division of progenitor cells as the number of cycles tends to infinity In our ... allowed the distribution of the time to division and differentiation of initiator cells to be potentially different from that of cells in subsequent generations Our time- lapse data provide the...
- 8
- 254
- 0
Tài liệu Instructions for Form 5405 (Rev. December 2011) First-Time Homebuyer Credit and Repayment of the Credit pdf
... Bureau of Intelligence and Research of the Department of State • Any of the elements of the Department of Homeland Security concerned with the analyses of foreign intelligence information Line E ... 50% in value of the outstanding stock of the corporation c A partnership in which you directly or indirectly own more than 50% of the capital interest or profits interest For more information ... purchased in 2008 If you checked the box on line 13a, enter the smaller of line 16 or line 17 on line 18 If you checked the box on line 13c or line 13d, enter the amount from line 16 on line 18...
- 6
- 589
- 0
Tài liệu Imaging of the nose and paranasal sinuses pdf
... SINUSITIS Orbital cellulitis or abscess ORBITAL COMPLICATIONS OF SINUSITIS CT MRI Cavernous sinus thrombosis C i h b i Intracranial complications of sinusitis Intracranial complications of sinusitis ... SINUSITIS (fluid level, complete opacity, mucosal thickening) SINUSITIS ( complete opacity maxillary sinus) SINUSITIS ( complete opacity sphenoid sinus) SINUSITIS ( complete opacity frontal sinus) ... sinus) Fungal sinusitis Fungal sinusitis Mucocele frontal sinus CT & MRI Frontal mucocele Mucocele Ethmoid sinus OSTEOMYELITIS ORBITAL COMPLICATIONS OF BACTERIAL SINUSITIS • Periorbital inflammatory...
- 47
- 478
- 1
Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot
... limiting Therefore, the effect of different activators of AMPK and the effect of inhibitors of protein phosphatases on the amino-acid-induced activation of ACC and p70S6K were examined in freshly ... glutamine Rapamycin is a potent inhibitor of mTOR and of the insulindependent activation of p70S6K in skeletal muscle [29–31] We compared the time- course of the effect of AICAr with that of rapamycin ... activation of both ACC and p70S6K, and one downstream of mTOR which inactivates p70S6K AICAr abrogates the amino-acid-induced activation of p70S6K indicating that AMPK is involved in the regulation of...
- 9
- 455
- 0
Báo cáo khoa học: Noninvasive imaging of microRNA124a-mediated repression of the chromosome 14 ORF 24 gene during neurogenesis potx
... allow the inclusion of neuronspecific exons in mature mRNA [16] LAMC1, which is one of the components of a heterodimeric molecule, laminin-1, comprising laminin a1, laminin b1, and laminin c1 subunits, ... review of the literature and determination of the neuronal correlation These included the following: c14orf24, laminin gamma (LAMC1), PTBP1, RNAbinding motif single-stranded interacting protein (RBMS1), ... analysis of c14orf24 expression in various cell lines: HT-ori3 cells (normal thyroid cell line), L132 cells (lung normal cell line), C6 cells (glioma cell line), CT-26 cells (colon carcinoma cell line),...
- 12
- 381
- 0
THE CONSTITUTION OF LAW Legality in a Time of Emergency potx
... in constructing the meaning of the text in light of the ideal The best account of this interpretative process is that of Ronald Dworkin He argues that judges who approach the interpretation of ... constitutive of a certain kind of power – of legal authority; and with the aid of that insight, we can also see why the different institutions should not be understood in terms of ‘competing supremacies’,5 ... institutions that are principally involved in bringing the principles of the rule of law to realization In fact, the Commonwealth constitution is just one example of institutional arrangement But...
- 268
- 661
- 0
THE CONSTITUTION OF LAW Legality in a Time of Emergency docx
... in constructing the meaning of the text in light of the ideal The best account of this interpretative process is that of Ronald Dworkin He argues that judges who approach the interpretation of ... constitutive of a certain kind of power – of legal authority; and with the aid of that insight, we can also see why the different institutions should not be understood in terms of ‘competing supremacies’,5 ... institutions that are principally involved in bringing the principles of the rule of law to realization In fact, the Commonwealth constitution is just one example of institutional arrangement But...
- 268
- 1,134
- 0
Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25