the vapor pressure of a pure liquid depends on which of the following
Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits potx
... O2affinity, K , can be derived for both the a and b subunits from the averaged parameters of HbA oxygenation (Table 1, Average). The associationand dissociation rate constants for the b subunitsare found ... afterphotodissociation, d, is calculated as the ratio of the quantum yield of BR, c, to that of the primary quan-tum yield of photodissociation, c0.Having the individual parameters of bimolecularoxygenation ... NaCl.Fig. 4. The parameters of O2binding to the a and b subunits within liganded hemoglobin as a function of NaCl concentration. Bars 1, 2 and3 are the relative changes in the association (kÂ),...
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Reliability analysis of a power system based on the multi state system theory
... 22.8ssRG=≥}Equation (1) indicates that when the capacity of the system is above 22.8 Ah, the system is reliable, though the capacity of a battery is lower than 5700 mAh. Suppose the capacity of the first ... reliability theory defines the power system and the batteries are binary, but they are all multi-state actually. The performance of the batteries can degrade, which results in performance degradation ... G is the capacity of the battery. 2150()2~ 6000,150GNTo analyze the reliability of the system by the traditional system reliability theory, we must gain the reliability of the battery...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
... Restriction siteW11F WT W11F CACCATGGCTAGAAAATATTTTGTCGCAGCAAACTTCAAATGTAA NcoIW168F WT W168F GAACCTTTATTCGCTATTGGTACCGGTAAA KpnIWT* W11F W11F ⁄ W168F GAACCTTTATTCGCTATTGGTACCGGTAAA KpnIY74W* ... calibration standards provided by the manu-facturer. Data processing was performed using the deconvo-lution module of the data analysis software to detect the multiple charge states and obtain ... maintaining the geometry of the active site. The availability of crystal structures of TIMs from 21 sources and the large database of TIMsequences from various sources facilitate an analysis of mutational...
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Tài liệu Báo cáo khoa học: Application of a fluorescent cobalamin analogue for analysis of the binding kinetics A study employing recombinant human transcobalamin and intrinsic factor pdf
... roughcomparison of the dissociation velocities indicated atleast a 10-fold faster liberation of the fluorescent ana-logue when compared with Cbl. The CBC dissociationspanned at least 90% of the total ... °C was incubated over time. Free ligandswere adsorbed on charcoal, and the absorbance spectrawere recorded. Concentration of appearing IF–CNCbl wascalculated by comparison with the standards ... respects. An alternativeexplanation seems to be more probable. Thus, distor-ted shape of the analogue causes partial corruption of its bonds with IF. As a consequence, the ligand and the protein...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... TCA TCATCA TCA TCA GGG CAC GAC ACA CAT CCC C; andreverse primer, GAT CGG TAC CTC ATT ACA GAGGAG GGA TAT GGG GAA C. The PCR reaction wasdone as described above. The amplified PCR product wasinserted ... was amplified from genomic T. reesei DNAwith the following primers: forward, GGG GAC AAGTTT GTA CAA AAA AGC AGG CTA TCA TGC TGT TGTCAG GTC CCT CTC G; and reverse, GGG GAC CACTTT GTA CAA GAA ... ⁄ TOF fragment ion analysis as well asby Edman degradation of the corresponding peptideafter purification by reversed-phase chromatography.During the search for and confirmation of the N-ter-minus...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... purification.Erythrocyte preparationBlood for HA assay was prepared as described byRavindranath et al. [12].Hemagglutination assayHemagglutination assays were performed in microtiterplates (Falcon) as recommended ... of animal glycoconjugate. They actas an important component of the ligands recognized by the lectins. Recognition can be affected by specific structuralvariations and modifications of sialic acids ... sialicacid may change with transformation or other alteration in the environment of the cell [63]. The normal human tissuescontain NeuAc, while malignant tumour cells containO-acetyl sialic acid...
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Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx
... extra-peroxisomal Aco1p.As mentioned previously, malate synthase catalyses the formation of malate from glyoxylate and acetyl-CoA, the source of the latter being either peroxisomal when breakingdown ... (Fig. 4A) .To examine whether a cytosolic malate synthase was asefđcient as a peroxisomal one for m aintaining a functionalglyoxylate cycle on oleic acid, liquid growth assays wereconducted. The ... cytosolicsupernatant. Equal fractions of each of the protein prepa-rations (10% of total vol) were i mmobilized on replicatemembranes to which were applied antibodies against Mls1por yeast peroxisomal...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
... amplicationbetweenC7UandC8L.Primersinitalicswereusedinthegenotyping of allele M.C1U5Â GAG AGG AAC TGA ACA GAA CAT TG 3ÂC1L 5Â CAA CTG CGT ATT AGT GAA GAA TG 3ÂC2U 5Â AAT CAA ATT TTA TTA TAA GAC C 3ÂC2L5Â AAT AGC TAA TTA GAG ACC AT 3ÂC3U 5Â GGT GTC AAA ... 5Â ATA CAC ACA AAC TCA CAA GG 3ÂMWU 5Â CAA CAT ATT TTA AAT AAA ATT GAC AAT 3ÂC9LM* 5Â ATA AAA ATG GTA TAC CTC ACT TGT*C 3ÂC9UM1 5Â TAA CAA TGA TTC TCT TTC TTT TAG 3ÂC9LM1 5Â AAT CTT TAT TTT ... TGG CAC AAC ATT GTA CAT TCT TGG G 3ÂC9U 5Â GTA TGG AAG TGT GGA ATA GTT T 3ÂC9L 5Â GGA CAC CAC AGA TAT CCA ATA G 3ÂC1011U 5Â CAT AAA ACT AAC AAT ACA TGT 3ÂC1011L 5Â TAG CAG ATA TTG AAA AGG AG...
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Tài liệu Báo cáo Y học: Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase pdf
... value almost 2.5-fold higher than the native cold adapted enzyme (Table 1). The mutantG26 1A/ Y26 9A exhibits an E a almost the same as in the caseofthenativeenzyme(Table1).Thermal inactivation ... andoverexpression of the gene encoding alkaline phosphatasefrom the Antarctic strain TAB5 [16]. Based on the crystalstructure (at 2.4 A ˚)ofanEscherichia coli alkaline phospha-tase variant ... with the aromatic ring of Tyr269, and theseunfavorable interactions could lead to a decrease of localflexibility and an increased E a value. The validity of the above interpretation was furtherreinforced...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... DR2:5Â-CCGTAAGGTCACAAGGTCACTCG-3Â,DR3:5Â-CCGTAAGGTCACAGAGGTCACTCG-3Â, DR4: 5Â-CCGTAAGGTCACAGGAGGTCACTCG-3Â, DR5: 5Â-CCGTAAGGTCACCAGGAGGTCACTCG-3Â. PAL0: 5Â-CGCAAGGTCATGACCTCG-3Â. One strand of each oligonucleotide wasannealed after ... [21]. A SmNR1 specific probe of 497 bp wasproduced by PCR amplification with TOPO 2.1-SmNR1 as a template (forward primer: 5Â-ATTTCAGAAGTTGAACAAACACAC-3Â, reverse primer: 5Â-AAGATGGTATTGAAGATGATGGTTGA-3Â), ... gene in mammalian cells suggests thatSmNR1 ⁄ SmRXR1 can interact with mammalian coac-tivators of transcription, and S. mansoni coactivators of transcription may have a similar mechanism toSmNR1...
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Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner doc
... toproduce acyl-CoA on the outer membrane of mitochondria.Acyl-CoA is then transported into the matrix forb-oxidation of acyl-group. Among the MACS familyproteins with the acyl-CoA synthetase activity, ... (5Â-GAATTCatgaaggttctcctccactg-3Â)and rO-MACS-XhoI-D (5Â-CTCGAGtgcccgtccccactcctggt-3Â)for o-macs; EcoRI-mMACS1-U (5Â-GAATTCatgcagtggctgaagagttt-3Â) and mMACS1-XbaI-D (5Â-TCTAGAtagctgaccaaactccttg-3Â)forMACS1, ... Yoshihara, Y., Kawasaki, M., Tamada, A. , Fujita, H., Hayashi,H., Kagamiyama, H. & Mori, K. (1997) OCAM: a new member of the neural cell adhesion molecule family related to zone-to-zoneprojection...
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Báo cáo khoa học: Characterization of a high-affinity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus ppt
... than the susceptibleweevil strains. Surprisingly, the microsomal fraction of anextract of the resistant insect D. melanogaster also displayed a strong binding capacity. The determination of the ... increasingconcentrations of 125I-labelled PA1b (0.35–3.2 nM).Table 2. Effect of proteinase K on the 125I-labelled PA1b bindingactivity on the microsomal fraction of S. oryzae WAA42 extract.Treatment a Binding ... post-translational cleavage of the albumin proprotein PA1, also releasing a second peptide(PA 1a) . PA1b consists of 37 amino acids, with six cysteinesinvolved in three disulfide bonds which confer the...
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Báo cáo khoa học: On the mechanism of a-amylase Acarbose and cyclodextrin inhibition of barley amylase isozymes pdf
... correspondingdissociation constants. In this equation, it was easier for a calculated constant to compare its value relative to zerorather than to obtain a large value. When the associationconstant value was ... discrepancy was explained by the higher affinity of acarbose for PPA as indicated by the lower dissociationconstant of the acarbose-PPA complex (1.7 lM)[24] .The dissociation constant of the acarbose-AMY ... lMdecreasing to 37.3 lMby addition of acar-bose. A 0is the AMY1 absorbance without acarbose, A is the absorbance measured at the above acarbose concentrations.(B) Reciprocal plot of the difference...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008