the unidentified hydrophobic motif kinase
Tài liệu Báo cáo khoa học: An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing pdf
... arrest the cells at the blebbing stage and thus halt the cell division cycle Therefore, the actual biological significance of the s-DAPK-1- and DAPK-1-induced membrane blebbing requires further ... in the Flag– s-DAPK-1 transfection lane was slightly smaller (Fig 2C, lane versus lane 1) This suggests that the depletion of the Myc tag only changes the size of the upper band, and that therefore ... supporting the existence of a protease that cleaves s-DAPK-1 protein in vivo The reason why the cleavage band was not observed in this assay may be the rapid degradation of the purified protein from the...
- 11
- 659
- 0
Tài liệu Báo cáo khoa học: Gas6 and protein S Vitamin K-dependent ligands for the Axl receptor tyrosine kinase subfamily pptx
... itself against and interact with the membrane, thus enabling the SHBG domain to bind to the receptor on either the same or another cell Axl, Sky and Mer in cancer The transforming activity of Axl ... [6] The crystal structure of the SHBG region of Gas6 reveals a V-shaped arrangement of LG domains with a hydrophobic patch and a calcium-binding site at their interface [7] (see also Fig 3A) The ... This was further strengthened by the recent observation that mouse protein S directly stimulated mouse Sky and Mer, both of which are present in the eye, and this was coupled to the potential...
- 14
- 600
- 0
Tài liệu Báo cáo khoa học: The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression ppt
... 2) and the absence of aromatic residues in the RNP and motifs The authors therefore proposed a novel class of protein recognition motif that they named U2AF homology motif (UHM) [25] The examples ... platform spread on the b-sheet from b4 to b2 in the 5¢)3¢ direction More often, the nucleotide at the 5¢ end of the central dinucleotide contacts the loops at the bottom of the b-sheet (loop and ... is provided by other non conserved elements of the RRMs The two most frequently observed elements are the protein side chains at the surface of the b-sheet (RNP position and the two adjacent...
- 14
- 428
- 0
Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc
... the intact organ to exclude the possibility that the data obtained with hepatocytes were restricted to the isolated cell system The anti-glucagon action of insulin was chosen to demonstrate the ... Inhibitors were added 10 before the agonists The incubation was terminated by rapidly aspirating the medium and immersing the dishes in liquid N2 Glycolysis and glycogen synthesis Materials and methods ... of 55 lL For the specific measurement of the atypical PKC isoenzyme-f, Ca2+ was omitted from the assay, 80 lgÆmL)1 phosphatidylserine was substituted for the kit lipid reagent and the kit peptide...
- 12
- 592
- 0
Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx
... between the levels and the production patterns of the (p)ppGpp in the two strains, either before or during the full stringent response (Fig 4B) Growth rates in the presence of kirromycin The effect ... the coiled-coil motif is directly involved in guanine nucleotide exchange The role of the coiled-coil motif The results of the present study demonstrate that the coiled-coil motif in E coli EF-Ts ... that the most essential regions for the activity of EF-Ts are located in the N-terminal domain and the core domain These observations are compatible with the absence of both the coiled-coil motif...
- 12
- 502
- 0
Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
... and a-tocopheryl nicotinate (TN) atherosclerosis, was further used together with LPS to strengthen LPS function MATERIALS AND METHODS Materials After removal of the culture medium for analysis ... humidified air Then, the medium containing serum was removed, and the cells were washed with phosphate buffered saline Next, mL of the medium containing TS without serum was added to the dishes After ... UÆmL)1 were also added to the dishes for induction of NO production Then, 48 h after the addition of LPS/IFN, the cells were subjected to various assays Nitrite analysis The amount of NO was determined...
- 6
- 494
- 0
Báo cáo khoa học: Identification of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis potx
... C-subunit Therefore, taken together, these results clearly indicated that mussel C-subunits are not generated as a consequence of the PTMs typical of the PKA C-subunit, but rather they differ in their ... are not scattered over the whole sequences, but rather limited to a particular region of the proteins On the other hand, the presence of a peak at 1605.8 Da was observed in the spectra of C2, C3 ... result of the in vivo deamidation of the Asn2 residue, and therefore the only difference between CA and CB was the presence of aspartic acid or asparagine, respectively, at position of their sequences...
- 11
- 509
- 0
Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
... at the last base, G to A, abolished the interaction of HIPPI, so the last motif would not interact with HIPPI The other two motifs might be the target of HIPPI In the upstream sequence of the ... significantly As shown above, the interaction of HIPPI with the mutated bp motif (G to T at the fourth position of the motif) was abolished, whereas the 60 bp sequence with the mutated motif exhibited substantial ... HIPPI interacted with the motif AAAGAGAAC () 963 to ) 955) We could not exclude the possibility that the other motif AAAG AAAAG () 418 to ) 410) present in the negative strand of the caspase-8 gene...
- 14
- 393
- 0
Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc
... rather reflects synchrony to other events of the spermatogenesis For instance, the mechanisms unleashing the pass of the haploid germ cells through the tight junctions from the adluminal to the ... has been argued that the germ cells themselves may signal the Sertoli cells their entry to meiosis, triggering their transport through the blood–testis barrier [43] On the other hand, apoptotic ... for the negative controls The resolution varied between and lmÆpixel)1 depending on the size of the section of the image The Adobe photoshop program was used to produce merged images in the double-staining...
- 15
- 389
- 0
Báo cáo khoa học: The interaction between casein kinase Ia and 14-3-3 is phosphorylation dependent pot
... other CKI isoforms if they contain a consensus 14-3-3 motif at the equivalent position of either residue 218 or 242 (Fig 4B), we therefore extended our analysis to the interactions between the ... than the other isoforms (Fig 3A) Although it is not possible to discern quantitatively the binding affinity for the g and c isoforms, as a result of the differing titres of the antibodies, there ... Chicago, IL, USA) The values shown are the percentage of the intensities of 14-3-3 captured by the peptide compared to the intensity of 14-3-3 applied to the beads (input) These results are taken...
- 14
- 344
- 0
Báo cáo khoa học: HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling ppt
... in the pAR[DRI] vector Of the 750 000 plaques analyzed, two of them hybridized with the probe The sequences of the inserted cDNA were identical In frame with the kgt11 Lac Z coding sequence they ... protein of the endoplasmic reticulum [22], calreticulin, a protein of the endoplasmic reticulum lumen [23], HIP/PAP, the RIIa and the Ca subunits of PKA were checked by immunoblotting in both the 100 ... compartment The locations of HIP/PAP and RIIa were further analyzed using a fractionation method (Fig 1B) The regulatory RIIa and the catalytic Ca subunits of PKA were detected in the 100 000...
- 9
- 310
- 0
Báo cáo khoa học: Identification of the heparin-binding domains of the interferon-induced protein kinase, PKR pptx
... at 200 mM salt Arrows indicate the positions of deletion mutants and the labels at the bottom of the panels show the residues retained in the deletion mutants (C) The region between 318 and 412 ... activity of the mutants The dsRNA-binding activity of the point mutants was tested by their binding to poly(I)Æpoly(C)-agarose The T lanes represent total proteins in the translation mix The B lanes ... binding performed at 200 mM salt Arrows indicate the positions of deletion mutants and the labels at the bottom of the panels show the residues retained in the deletion mutants (B) A second region between...
- 15
- 424
- 0
Báo cáo Y học: Human sprouty 4, a new ras antagonist on 5q31, interacts with the dual specificity kinase TESK1 potx
... M93.007 of the Netherlands Heart Foundation, The Hague, the Netherlands and the Fonds National de la Recherche Scientifique Bekales, Brussels, Belgium REFERENCES Hacohen, N., Kramer, S., Sutherland, ... i.e 87% at the DNA and 88% at the protein level Our novel gene was therefore named human spry4 (hspry4) Because multiple tissue Northern blotting revealed that the 4.9-kb transcript is the predominant ... sequences [23], two Alu-repeats and three CAGAC motifs [24] BLAST searches revealed the homology of the coding sequences of the 4.9- and 7-kb cDNAs with the sprouty (spry) gene family Homology with...
- 11
- 542
- 0
Báo cáo hóa học: " Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a " pptx
... NBS1 recruits the ATM kinase to DSB sites [15], and NBS1 [15] and ATM [16] are then both required to recruit the ATR kinase [16] Activation of the ATM and ATR kinases allows them to phosphorylate ... conflicting data in the literature about the roles of the ATM and ATR kinases Although many publications demonstrated the participation of other NHEJ proteins in PIR [3,5,6,8,25,26], the precise roles ... proteins are recruited to the integration site (5) These proteins, and likely other factors, then mediate the other steps of post integration repair, which require possibly further end processing,...
- 12
- 398
- 0
Báo cáo hóa học: " The first hydrophobic region of the HPV16 E5 protein determines protein cellular location and facilitates anchorage-independent growth" potx
... lie in the large amounts of E5 protein synthesised from the codon-adapted genes, which will probably accumulate in the ER with the cell being unable to further transport the protein to the Golgi ... cloned into the vector pFlag-CMV4 (Sigma) Cloning was performed deleting the methionine start codon of the viral protein and ligating the rest of the E5 gene the vector Flag epitope The HPV16 E5 ... respectively The observation that the presence of the first hydrophobic region is necessary for localisation of the protein to the ER, early endosomes or lysosomes is noteworthy, mainly because of the...
- 10
- 291
- 0
Báo cáo sinh học: "At the crossroads: AMP-activated kinase and the LKB1 tumor suppressor link cell proliferation to metabolic regulation" pdf
... deactivation of the kinase, and AMP potentiates phosphorylation and activation of AMPK by an upstream kinase AMPKkinase (AMPKK; Figure 1) [1] The existence of an AMPKK was suggested by the observation ... Biology 2003, to mammalian calcium-calmodulin kinase- kinases (CaMKKs) and to the tumor suppressor kinase LKB1 But although CaMKK can weakly phosphorylate the AMPK ␣ subunit at Thr172, partially purified ... Characterization of the AMP-activated protein kinase kinase from rat liver, and identification of threonine-172 as the major site at which it phosphorylates and activates AMP-activated protein kinase J...
- 4
- 247
- 0
Báo cáo y học: "Tumour necrosis factor activates the mitogen-activated protein kinases p38α and ERK in the synovial membrane in vivo" doc
... and TNF-α has the potential to signal through all of them [17,18] Therefore, each of these different MAPKs is a potential therapeutic target In the present study we investigated the effect of ... activation of the three MAPK families (ERK, JNK and p38MAPKα) Moreover, we defined the cell types in the synovial membrane that exhibit MAPK activation and investigated the effects of anticytokine therapies ... vated protein kinase (MAPK)α, (b,e,h) extracellular signal-regulated kinase (ERK), and (c,f,i) c-Jun amino-terminal kinase (JNK) were stained in both the synovial pannus (panels a–c) and the synovial...
- 8
- 319
- 0
Báo cáo y học: " Local treatment with the selective IκB kinase β inhibitor NEMO-binding domain peptide ameliorates synovial inflammation" pot
... large therapeutic study in which AA was induced in rats (n = 15/ group) on day At the start of arthritis symptoms (day 10), the animals received an i.a injection with either the NBD peptide or the ... conclusion, these experiments demonstrate the effectiveness of the NBD peptide in human cells Discussion In the present study, we show for the first time that i.a administration of the highly ... reduced In these synovial biopsies, the microarchitecture of the synovium is preserved, allowing investigators to study the effects of the NBD peptide on synovial inflammation in the complex,...
- 9
- 286
- 0
báo cáo khoa học: " The Arabidopsis EAR-motif-containing protein RAP2.1 functions as an active transcriptional repressor to keep stress responses under tight control" docx
... (E) residues are indicated The key residues of the EAR -motif without (wEAR -motif) or with site-mutation (mEAR -motif) are also shown (B) RAP2.1 binding to the DRE element The oligo-nucleotide probes ... the expression results were consistent with the ChIP results For the LUC reporters driven by the DRE fragments of the RD/COR gene promoters, RAP2.1 was able to repress the basal activity of the ... RAP2.1 itself Therefore, the harmonious operation of the DREB-type activators and the RAP2.1 repressor maintain the activation of the RD/ COR genes at an appropriate level and the plant stress...
- 15
- 277
- 0
Báo cáo y học: "Increased plasma levels of the soluble Mer tyrosine kinase receptor in systemic lupus erythematosus relate to disease activity and nephritis" pot
... participated in the design of the study, in the analysis of the patient cohorts, in the statistical analysis and in the writing of the manuscript BD initiated, planned and supervised the study, participated ... engulfment of the apoptotic cell [30,31] The process requires that the Mer is an intact membranespanning protein and depends on activation of the intracellular kinase domain of Mer The presence ... Foundations, the Ernhold Lundström Foundation, the Albert Påhlsson Foundation, the Hulda Ahlmroth Foundation, the Swedish Rheumatism Association, Swedish Society of Medicine, the Foundation of the National...
- 9
- 372
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008