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Tài liệu Notes on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products doc
... Note: The correct NVP setting is necessary; it has an influence on the determination of the cable length by the instrument Notes on the use of hand- held test devices for installation of the KRONE ... Note: The correct NVP setting is necessary; it has an influence on the determination of the cable length by the instrument Notes on the use of hand- held test devices for installation of the KRONE ... Note: The correct NVP setting is necessary; it has an influence on the determination of the cable length by the instrument Notes on the use of hand- held test devices for installation of the KRONE...
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Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf
... protein for MS analysis of the myristoylation site, we used 293T cells as the expression system, as the presence of the SV40 large T antigen in these cells enhances their resistance to apoptosis Apoptosis ... attached to the culture dish Furthermore, cells expressing the JSP1-G2A mutant remained attached to the dish despite the fact that the mutant protein was expressed at similar levels to those of the wild-type ... expression of the myristoylation mutant These results suggest that JSP1 induces apoptosis when overexpressed in cells, and further demonstrate the importance of myristoylation for this functional...
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Tài liệu Báo cáo Y học: The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex potx
... that the region surrounding Arg252 is of importance for the formation of the oligomeric enzyme complex, which is a prerequisite for obtaining a fully active enzyme [8,15] Upon removal of this entire ... in a region within the catalytic domain This is the first evidence that this region is involved in the formation of the active complex MATERIALS AND METHODS transfected with the pcDNA3 vector alone ... critical for the association properties of the enzyme The nonassociated form is inactive For purified human TPP II and recombinant murine TPP II, it has been shown that the smallest active form of...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... We propose that glycosylation is important for the folding of HFE and is essential for transport and exit of the protein from the ER The importance of glycosylation is cumulative, however, with ... further explore this possibility The molecular basis for the observed deficiency in b2M binding of the triple mutant remains unclear One possibility is that glycosylation deficiency compromises ... towards a mixed phenotype for the partially glycosylated mutants Therefore, although cells expressing either the double or single mutants showed incorporation into the ERC and punctate patterns...
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Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot
... (F) by the enzyme are shown by arrows In these panels, only the sequences of the oligonucleotides cleaved by the enzyme are shown The differences in the lengths of the arrows reflect relative ... demonstrated for mouse RNase H1 that the HBD is required for processivity of the enzyme [24] Tma-RNase HI did not show the processivity for cleavage of the R12 ⁄ D12 substrate (Fig 6) However, this result ... residues, the scissile phosphate group of the substrate and water molecules, a two-metal ion catalysis mechanism has been proposed for RNase H [14,16,17] According to this mechanism, one metal ion is...
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Báo cáo khoa học: YidC is required for the assembly of the MscL homopentameric pore potx
... unaltered upon YidC depletion (Fig 4A, left panel) The reason for this discrepancy is not clear, but might be explained by the structural constraints of the respective mutants used for the assays The ... membranes is equal to that of the non-depleted IMVs This indicates that YidC is required for assembly of the MscL complex (Fig 5) To investigate the role of the Sec translocon in formation of the MscL–HA ... functioning is critical for assembly of the ATP synthase subunits rather than their individual insertion into the membrane [26] If neither YidC nor the Sec machinery is absolutely required for membrane...
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Báo cáo khoa học: The F13 residue is critical for interaction among the coat protein subunits of papaya mosaic virus doc
... critical for interaction among the CP subunits monomeric form of the protein [10] This protein failed to assemble, form disks or interact with RNA in vitro [10] On the basis of this result, we hypothesized ... As the F13L and F13Y mutants form only NLPs in E coli, we needed to disrupt NLPs using the widely employed acetic acid treatment to isolate the disks as previously described [3], to test their ... CP F13 Therefore, on the basis of our results, it is likely that a deletion of 32 amino acids will be tolerated by PVX without disturbing the assembly process Alignment of this F residue is also...
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Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
... ASK1-induced apoptosis in vitro [28] Furthermore, the ATP-binding domain of HSP70 is critical for sequestering AIF in the cytosol [29] In the present study, we demonstrated that the ATP-binding domain ... expression of the anti-apoptotic protein, Bcl-2 [33] On the basis of the available evidence, HSP70 and HSP70DPBD may also suppress Smac release and apoptosis by regulating the expression of these pro-apoptotic ... of HSP70, rather than the peptide-binding domain, was essential for this specific function of the protein Recent studies have revealed that HSP70-mediated protection is essential for cells aiming...
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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
... sensitivity of the human enzyme, and showed that the variant, Glu17, in the pig enzyme is responsible for its peculiar kinetic characteristics (low carnitne Km and high malonyl-CoA IC50) This is the first ... carnitine: mm (for standard comparison with other published data), and a concentration equal to the Km for carnitine of each enzyme (for comparison between mutants) In this article, we show that Glu17 ... In this model, Asp17 does not face the C-terminal fragment A possible explanation for this is that, in the case of CPT1B, the docking of the N-terminal fragment might differ from that of the established...
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Báo cáo khoa học: Passage through the Golgi is necessary for Shiga toxin B subunit to reach the endoplasmic reticulum pptx
... used this time-course as a basis for our further experiments Passage through the Golgi is required for StxB to reach the ER in cytoplasts We wished to test directly if passage through the Golgi ... to the media for and chase in unlabeled media for the final 25 of the assay before fixation to illuminate the endocytic pathway Surprisingly, after 120 min, although the majority StxB (red arrows) ... for times shown (A) Shiga and Tfn (green) internalized together for 10 then chased for 10 or 30 For 120 min, Tfn was internalized for the final 25 (B) Cytoplast with StxB (red) immunolabeled for...
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Báo cáo khoa học: Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum PSI-J is required for efficient electron transfer and stable accumulation of photosystem I pot
... template (C) Northern blot showing that there is no wild-type-sized psaJ mRNA (as a loading control the left hand side shows the stained and the right hand side the actual Northern blot) (D) ... verification of one of the homoplastomic psaJ knock-out lines is shown Northern blot analysis was also performed to demonstrate that the psaJ gene was disrupted by the insertion of the aadA cassette ... centrifugation as shown in Fig did not display the 2–3 nm blueshift (data not shown), indicating that the blue shift is caused by excess free Lhca complexes in the thylakoid membrane This was further supported...
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Báo cáo khoa học: The b domain is required for Vps4p oligomerization into a functionally active ATPase potx
... This study This study This study This study This study This study This study This study This study This study This study This study This study This study This study This study This study This ... Although this finding was unexpected, there is a precedent for the role of regions outside of the AAA domain in oligomerization For example, the linker region between the two AAA domains of p97 is required ... role in the formation of a Vps4p ATPase complex that is functional in vivo The formation of the latter may be a prerequisite for interaction with Vta1p Furthermore, our data highlight the importance...
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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc
... evidence supporting this idea As shown for the Erv2 protein, the dimer formation depends on an interaction of the CGC motif with the CXXC motif of the opposite monomer The short form of the Erv1 ... cysteines in the crystallized part of Erv2p is separated by 17 residues and the exact distance is found between the corresponding cysteines of Erv1p The reason for this exact match is clear from the structure ... arm in the related Erv2p [11] In one conformation, this arm brings the CGC close to the redox-active centre of the other monomer, while in the second conformation the arm reaches out to the open...
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Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt
... MREa is also the most crucial MRE for transcription of the MT gene and that there is a relationship between the distance from each MRE to the TATA box and their influence on promoter activity of the ... mediates the export of copper from the cytosol, similar to the P-type ATPase [14] On the basis of the fact that MREs mediate the transcriptional response of the MT gene to heavy metals [10,12], the ... both the 82- and 70-kDa proteins were visualized by silver staining (unpublished data) In contrast, only the 70 kDa subunit was observed by South-Western blot analysis (Fig 3A) This is consistent...
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The Goal Setting ReportA 6-Step Plan to Achieving Your GoalsBy Paul Smithson Smashwords Edition Copyright 2013 Paul Smithson ###Smashwords Edition, License NotesThis ebook is licensed for your personal enjoyment only. This ebook may not be re-sold or pot
... to influence their decision in the correct way Obviously, if there is no possibility of being promoted to Senior Manager then this is not an actionable goal For example, if there is only one Senior ... the realities list you put together in step and the list of options you put together in step Taking both of these into account you can work out a wellinformed way forward This ‘way forward’ will ... scale There is a reason for that Franklin believed that his list of virtues should be constantly at the forefront of his thinking and so every one of his virtues was a daily goal So for example the...
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Credit for the Elderly or the Disabled For use in preparing 2012 Returns docx
... Tax Guide for Seniors Form (and instruction) Schedule R (Form 1040A or 1040) Credit for the Elderly or the Disabled See How To Get Tax Help, near the end of this publication, for information ... for information about getting this publication and form Are You Eligible for the Credit? You can take the credit for the elderly or the disabled if you meet both of the following requirements You ... take the credit for the elderly or the disabled Start Here Were you 65 or older at the end of the tax year? Yes No No You are a qualified individual and may be able to take the credit for the...
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Báo cáo khoa học: Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection pdf
... Data for the loop mutants further demonstrated that the mcaBk substrate was distinct (Table 1) This substrate showed only little to no change in activity with the loop Gly mutants Only G611A, the ... ˚ studies [17], is more than A from the active site The closed form orients the phenol oxygen of this residue within hydrogen bonding distance from the carboxyl group of the scissile peptide bond ... that the Tyr605 hydroxyl is in close proximity to the carbonyl of the scissile peptide bond Therefore, Tyr605 is probably also responsible for transition stabilization, suggested previously for the...
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Báo cáo khoa học: A novel factor XI missense mutation (Val371Ile) in the activation loop is responsible for a case of mild type II factor XI deficiency doc
... results are consistent with the FXI:C and FXI:Ag measured in the patients plasma, further supporting the hypothesis that the Val371Ile mutation is a 6130 type II defect leading to the production ... in the activation loop at the N-terminus of the catalytic domain, are notable In the zymogen FXI, Ile371 is exposed to the solvent, while in the activated FXI it is inserted into the protein The ... does not form many contacts with other residues (Fig 6) Therefore, the additional methyl in the Val371Ile mutant probably does not disturb the structure and the domain rearrangement in the zymogen...
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Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis pot
... during pupal stages Of the control flies that contain the either FLAG–mjmj (line 35) transgene or the hsp70–GAL4 driver under the background of the djmj mutation, 10.7–14.7% of pupae showed the abnormal ... responsible for the content or functionality of any supplementary materials supplied by the authors Any queries (other than missing material) should be directed to the corresponding author for the article ... results were obtained for the Df(3L)AC1 chromosome, which lacks a djmj locus in the genome These results suggest the involvement of dJmj in the establishment and ⁄ or maintenance of the closed chromatin...
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Báo cáo khoa học: Direct CIII–HflB interaction is responsible for the inhibition of the HflB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx
... or CIIIC The bands corresponding to r32-Chis (1.2 lM) remaining after proteolysis with GST–HflB (0.8 lM) for 40 in the presence of His6–CIII or CIIIC (up to 200 lM) are shown in the upper panels ... apply only for CII? We examined the mode of the inhibitory action of CIII on proteolysis of the heat shock sigma factor r32, another substrate of HflB Like proteolysis of CII, r32 proteolysis requires ... substrates, for which the mechanism of proteolysis is probably somewhat different [21] Whether kCIII would act as an inhibitor for such substrates (e.g SecY, YccA, Foa) remains to be seen However, the...
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