standardized isolation of human mesenchymal stromal cells with red blood cell lysis
Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... relative number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal ... was transferred to tubes containing the same volume of mol/l NaOH The cells were immediately washed with PBS twice and solubilized with a cell lysis buffer for protein assay The cell lysis buffer ... Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 J Cell Biochem 1997,...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal ... Research (IZKF) Würzburg References Kolf CM, Cho E, Tuan RS: Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche, selfrenewal and differentiation Arthritis Res Ther ... Steinert et al Introduction Mesenchymal progenitor cells, also referred to as mesenchymal stem cells (MSCs), provide an attractive alternative to chondrocytes with regard to cell- based approaches to...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells ... increased the percentage of proinsulin and insulin expressing cells with the maximum effect seen in cells treated with LAM (Figure 2) These data are consistent with the mRNA levels of Insulin and Glut2...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... mineralized cells and the clean nanofibers, respectively; (D) Enlarged view of cells with an aggregation of minerals; (E) Energy-dispersive Xray spectrum of the bone minerals on mineralized cells (as ... the most abundant cells of bone tissue, making up 95% of all bone cells Relative to the other bone cells, the osteocytes are long lived, up to 25 years, compared to months of the osteoblasts ... 28 days of culture with human MSCs The calcium content was normalized by the number of viable cells Significant difference of investigated groups was denoted as * (p < 0.05) For the cells on...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 1991;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007 ;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche, ... stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009 ;Human mesenchymal stem cells express vascular cell phenotypes upon interaction with endothelial cell ... differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal...
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Analyzing the immunobiology of cultured mesenchymal stromal cells
... characteristics of stem cells 1.2.4 Classification of stem cells 1.3 WHAT ARE MESENCHYMAL STROMAL CELLS 1.3.1 Definition of mesenchymal stromal cells 1.3.2 Heterogeneity of mesenchymal stromal cells 10 ... on mesenchymal stromal cells by immune system 1.4.4 Summary 1.5 36 38 APPLICATION POTENTIALS OF MESENCHYMAL STROMAL CELLS 39 1.5.1 Application of mesenchymal stromal cells 1.5.2 Limitation of mesenchymal ... colony-forming cells [Lanza 2001], bone marrow stromal cells [Ciapetti 2006], multipotent adult progenitor cells, mesenchymal stem cells, tissue committed stem cells, pluripotent stem cells [Ratajczak...
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Báo cáo y học: "Controlling Osteogenesis and Adipogenesis of Mesenchymal Stromal Cells by Regulating A Circadian Clock Protein with Laser Irradiation" pptx
... differentiate from mesenchymal stromal cells (MSCs) MSCs are multipotent cells that can replicate as undifferentiated cells and that have the potential to differentiate to lineages of mesenchymal tissues, ... Histochemical analysis For immunostaining of mCRY1 or mPER2, cells were fixed with 4% formalin in phosphate buffered saline (PBS, pH 7.4) 24 hrs after irradiation Fixed cells were incubated with a primary ... light for 30 in the presence of 5% silver nitrate and then incubated with 5% sodium thiosulfate for The stained cells were rinsed with deionized water For ALP staining, cells were stained using naphthol...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines Journal of Translational ... coculture with fresh, autologous T cells in the presence of T cell stimuli Use of irradiated tumor cells in cocultures yielded comparable suppressor cell induction, suggesting that tumor cells need ... CD33+ or CD11b+ cells are subsequently co-cultured with fresh, autologous CFSE-labeled T cells at a 1:4 ratio in the presence of T cell stimuli After days, T cell proliferation is measured as CFSE-dilution...
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báo cáo hóa học:" Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations" pptx
... of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Dose-response effect of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Cells were incubated with ... proliferation of human NP cells (10%FBS/F12) (n = 20) Figure Dose-response effect of growth factors on proliferation of human NP cells (10%FBS/F12) (n = 20) Cells were incubated with increasing ... The morphology of human NP cells under 1%FBS conditions The morphology of human NP cells under 1%FBS conditions 1%FBS, 100 μg/L IGF-1, NP cells showed an irregular morphology with low mitotic...
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Báo cáo y học: "Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells" pdf
... epitope profile of tonsil-derived mesenchymal progenitor cells (T-MPCs) (a) Immunofluorescence analysis of cell surface epitope profiles of T-MPCs and bone marrow-derived mesenchymal progenitor cells ... of human bone marrow mesenchymal stem cells Stem Cells 2006, 24:386-398 D'Ippolito G, Schiller PC, Ricordi C, Roos BA, Howard GA: Agerelated osteogenic potential of mesenchymal stromal stem cells ... peripheral blood mononuclear cells (PBMCs) (105 cells) were incubated for days with either μg/mL PHA or allogeneic stimulating PBMCs (1 × 105 cells) with or without bone marrow-derived mesenchymal...
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Báo cáo y học: "Signatures of human regulatory T cells: an encounter with old friends and new" pdf
... experiments, red blood cell depleted splenocytes from BALB/c mice were labeled with anti-CD4 and anti-CD25 Labeled cells were separated with a MoFlo and purity was in excess of 98% Isolated cells were ... phenotype and the amount of Foxp3+ cells within MACS purified human CD4+CD25+ T cells (Additional data file 5); and a Word table describing the phenotype of contaminating cells within MACS purified ... proliferation of TRegcells It is an activation-induced homo-dimeric glycoprotein receptor on T cells that interacts with the B7 ligands on the surface of antigen-presenting cells (APCs) The mechanism of...
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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
... Statistical Analysis 87 Chapter – Coculture of Human Umbilical Cord Blood Endothelial Colony-Forming Cells with Human Fetal Mesenchymal Stem Cells for the Generation of Vascularised ... Proposed Cell Type – Human Fetal Mesenchymal Stem Cells 50 2.8.2.5 Endothelial Cell Types 51 2.8.2.6 Endothelial Progenitor Cells 52 2.8.2.7 Endothelial Progenitor Cells in ... Sprouting of the endothelial cells is induced, resulting in tip cells migrating towards the hypoxic stimulus, with stalk cells following behind New vascular network forms with the emergence of various...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... dark at room temperature with ml of DMSO One hundered microliters of the supernatant was later transferred into a 96-well flat-bottomed cell culture plates, which was measured spectrophotometrically ... correlated to loss of cell viability upon exposure to low temperature It was observed that some cells detached after exposure to low temperature Virtually all of the detached cells were determined ... culture within bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the...
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Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx
... course of the effect of pyrrophenone on the proliferative capacity of U937 cells The cells were incubated with (closed bars) or without (open bars) lM pyrrophenone for the times indicated, and cell ... form of mixed micelles produced using Triton X-100 (B) Time course of the effect of BEL on the proliferative capacity of U937 cells The cells were incubated with (closed bars) or without (open ... Time course of the effect of MAFP on the proliferative capacity of U937 cells The cells were incubated with (closed bars) or without (open bars) 10 lM MAFP for the times indicated, and cell number...
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Báo cáo khoa học: Reaction of human UMP-CMP kinase with natural and analog substrates docx
... elution volume of UMP-CMP kinase indicated a Stokes radius of 2.8 nm, in agreement with a monomer species Expression and purication of human UMP-CMP kinase as a fusion protein with GST BL21 gold ... N-terminus of UMPCMP kinase [4] Cells were grown in 2YT medium [17] containing ampicillin 100 lgặmL)1 until D600 ẳ 0.4 After induction with mM isopropyl thio-b-D-galactoside and growth for h at 22 C, cells ... (Amersham Biosciences) (100 mg protein for mL of gel), washed with 50 mL of buer A and 10 mL of buer A containing 0.3 M NaCl, before elution of the protein with buer A containing M NaCl Further purication...
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Báo cáo khoa học: Functional association of human Ki-1⁄57 with pre-mRNA splicing events docx
... colocalization of Ki-1 ⁄ 57 with SMN ⁄ GEMS in Adox-treated cells (Fig 7Biii) Despite the diffuse nuclear distribution of EGFP–Ki-1 ⁄ 57, the majority of the red GEMS spots coincide with spots of brighter ... intensity of each band Microscopy analyses For the subcellular localization assays, HEK293 cells were grown on glass coverslips with the required culture medium The cells were fixed with NaCl ... body with which Ki-1 ⁄ 57 is associated in HEK293 cells treated with Adox We, like others before us, observed also that Cajal bodies colocalize with nucleoli in some cells [31] (not shown) Subcellular...
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Atlas of Human Pluripotent Stem Cells doc
... later demonstrated that ESC-like cells can be isolated from human embryos They described the isolation of ICM cells from human blastocysts and the culturing of these cells for two passages, while ... research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass blastocyst ... Methods for the Derivation of Human Embryonic Stem Cell Lines Abstract Human embryonic stem cells (hESCs) are pluripotent cells derived from the inner cell mass (ICM) of the developing embryo They...
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Báo cáo khoa học: MicroRNA-23b mediates urokinase and c-met downmodulation and a decreased migration of human hepatocellular carcinoma cells doc
... correct orientation of the insert was verified by sequencing Transient transfection of SKHep1C3 cells with miR-23b and transient transfection of AB2 cells with anti-miR-23b Molecules of dsRNAs that ... amount of miR-23b, less than that expressed by normal AB2 cells The HepG2 cells with a c-met mRNA amount comparable to that displayed by SKHep1C3 cells expressed miR-23b at levels as low as those of ... c-met protein expression in SKHep1C3 cells (A) Western blot analysis of uPA in the conditioned media of control cells (lanes and 4) and miR-23b-transfected cells, at 48 h and 72 h after transfection...
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báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
... incubated with allogeneic T -cells stained with CFSE After days of co-culture, percentages of CD3+ dividing T cells measured by flow cytometry were linearly correlated with the loss of CFSE fluorescence ... cells and AAV vectors are mainly episomal A single exposure of pDC to rAAV2/1, rAAV2/2 or rAAV2/5 led to very low levels of transduced cells ranging from around 3% to less than 1% of E-GFP+ cells ... transduction of hematopoietic cells [30-32] The elongation factor-1α (EF1α) and phoshoglycerate kinase (PGK) promoters were shown to have an activity in a human CD34+ cell and in cultured cord blood cells...
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