speci c uptake into cells
Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... reducing hMS and returning it to the catalytic cycle The turnover of hMS increases as MSR is reduced to the one-electron, two-electron and fourelectron reduced states, reflecting a higher concentration ... S Scrutton Formation of holo-methionine synthase Co Homocysteine Methionine Cob(I)alamin Methylcob(III)alamin Primary turnover cycle AdoHyc AdoMet H4folate CH3H4folate Co Cob(I)alamin e– Cob(II)alamin ... of reducing equivalents needed to return hMS to the catalytic cycle MSR was shown previously to reduce AqCbl to cob(II)alamin, and this activity is thought to facilitate the uptake of cobalamin...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... the complicated relationship between ECs and non-ECs such as mural, hematopoietic and mesenchymal fibroblast cells, even though a conditional genetic modification such as endothelium -speci c knockouts ... same Tie2–Cre transgenic mouse line, which showed that recombination occurred in hematopoietic cells as well as ECs [27], and that cardiac valvular cells were derived from endothelial cells through ... (MACS) columns and MACS goat anti-rat IgG microbeads (Miltenyi Biotec, Bergisch Galdbach, Germany) were used according to the manufacturer’s protocol Attached cells were trypsinized, collected...
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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf
... annealed oligonucleotides 5¢-CTAGTCGTCCGAACTCCGATAATCGC CGTCAGGGCGGTCGCGAACGTTTAG-3¢ and 5¢-CA TGCCAAACGTTCGCGACCGCCCTGACGGCGATTA TCGGAGTTCGGACA-3¢ into the unique SpeI restriction site p13R4-DP ... constructing p13R4-P, except that the following primers 5¢-ACTCATA CTAGTCTTAGCCATGGCTTCCCGCCG GCG-3¢ and 5¢-CCATCCGAATTCTCACTACACATTGATCCTAGCA GAAGC-3¢ were used for the PCR amplification of the C- terminal ... primers 5¢-GTCTGGCGGAA AACCTCAGTGTGACGC-3¢ and 5¢-GACACCAGACCA ACTGGTAATGGTAGCGACCG-3¢ were used for the amplification of the 3¢ end of the b-gal gene The presence of similar amounts of genomic DNA in...
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Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... 5¢-GGCCCAG GTGACTCAGCTATT-3¢; reverse, 5¢-AGGGCATCCGA GAATTCCTT-3¢), LAMP2 (forward, 5¢-TCAGCATTGC AAATAACAATCTCA-3¢; reverse, 5¢-CAGTCTGCTCT TTGTTGCACATATAA-3¢), CTGF (forward, 5¢-CA AGCTGCCCGGGAAAT-3¢; ... 5¢-GGACCAGGCA GTTGGCTCTA-3¢), JUN (forward, 5¢-GGATCAAGGC GGAGAGGAA-3¢; reverse, 5¢-TCCAGCCGGGCGATT-3¢), PLAU (forward, 5¢-CTGTGACCAGCACTGTCT CAGTTT-3¢; reverse, 5¢-CCCAGTGAGGATTGGATGA ACTA-3¢), ... 5¢UGACUUCCCUGGCCUAUUUUU-3¢, 5¢-ACAUUGAGC UCCUCUCUUGUU-3¢, 5¢-GAUAAGGUUGCUUCAGU UAUU-3¢ and 5¢-ACAGUACGCUAUGAAACUAUU-3¢, respectively As a negative control, we used an NT siRNA (5¢-UAGCGACUAAACACAUCAA-3¢) or a RISC-free...
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Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... Tanaka S (1990) Methods of successive multiparametric cytochemistry and microfluorometry on identical cells with special reference to cell cycle phases in a chick embryo Exp Cell Res 186, 6–14 32 Tanaka ... bottle by shaking were collected and named mitotic (M) cells Residual cells were collected and named G2 cells These cells and logarithmically growing cells were separated into Triton-soluble (S) ... transitions in the cell cycle are also required to prevent re-replication of DNA in a single cell cycle Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7]...
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Báo cáo khoa học: Cell type-specific transgene expression of the prion protein in Xenopus intermediate pituitary cells ppt
... product was detected in the anterior pituitary cells This cell -speci c induction of GFP–PrPC expression occurs because when the frog is adapted to a black background the POMC promoter becomes ... transgenic for GFP–PrPC showed fluorescence speci c in the melanotrope cells of the intermediate pituitary and in these cases we never observed nonspeci c anterior pituitary or brain fluorescence GFP–PrPC ... melanotrope cells constitute a homogeneous population of strictly regulated neuroendocrine secretory cells and their biosynthetic and secretory activity can be physiologically manipulated by simply placing...
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Báo cáo khoa học: The specific delivery of proteins to human liver cells by engineered bio-nanocapsules doc
... EGFP fluorescence was speci cally observed in human hepatocellular carcinoma HepG2 cells and NuE cells, whereas no EGFP fluorescence was observed from either human colon adenocarcinoma WiDr cells or ... nontransfected Cos7 cells (a), ng of L-FLAG-EGFP particles from the extracts of transfected Cos7 cells (b) and ng L(n45)FLAG-EGFP particles from the extracts of transfected Cos7 cells (c) were ... procedures Cell cultures Human hepatoma HepG2 cells, human squamous cell carcinoma A431 cells and human colon adenocarcinoma WiDr cells were cultured in Dulbecco’s modified Eagle medium (DMEM), supplemented...
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Báo cáo khoa học: Specific TSC22 domain transcripts are hypertonically induced and alternatively spliced to protect mouse kidney cells during osmotic stress pptx
... 2B tttttccag ⁄ TGCATC Exon tttttccag ⁄ TGCATC Exon tttttccag ⁄ TGCATC Exon tcttcacag ⁄ GATCTG Exon we exposed mIMCD3 cells to either of those stimuli alone and to a combination ... splice variants are indicated by arrows below the schematic representation of genomic DNA (B) Products of PCR amplification using splice variant -speci c TSC22D2 PCR primers (C) Nucleotide sequence ... GAAATGTTGTCCACAAGAGTGTC (forward; initiation codon in bold-type) and TGCTGAGGAGACATTCGG CTG (reverse) and the correct sequence of the PCR product was confirmed by double-pass sequencing pcDNA5 ⁄...
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Tài liệu Báo cáo khoa học: Bacitracin is not a specific inhibitor of protein disulfide isomerase pptx
... evidence for the in vivo speci city of bacitracin (or potential products) for PDI inhibition Our results clearly show that bacitracin in vitro is not a speci c inhibitor of PDI, but that it interacts ... the presence of bacitracin First, there is a net increase in fluorescence due to the bacitracin However, with excitation at 280 nm and emission at 350 nm, bacitracin is much less fluorescent on a ... Pages N, Bac P, Bordet R & Vamecq J (2009) Experimental stroke protection induced by 4-hydroxybenzyl alcohol is cancelled by bacitracin Neurosci Res 64, 137–142 25 Cho J, Furie BC, Coughlin SR...
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Tài liệu Báo cáo khoa học: The cartilage-specific transcription factor Sox9 regulates AP-2e expression in chondrocytes pptx
... 5¢-GAGGCCAGCGAAGA ATAGTG-3¢, and Sox9_1prom_rev, 5¢-GTTCTCTC CCTTTTCCCCAGC-3¢ (234 bp fragment); Sox9_2prom_ for, 5¢-CAGTCACTCAACAGTCTCTGG-3¢, and Sox9_2 prom_rev, 5¢-CACTTCGCTCTCAGGCTTC-3¢ (213 ... oligomeric binding sites for Sox9, speci c for the AP-2e promoter (Sox9_1, 5¢-GCGG CTCTGATCAATGTGGGCTGAATTC-3¢; and Sox9_2, 5¢-CATGCCCACACTCAATCAGCCCAGGACCC-3¢) were generated The fragments correspond ... mutSox9-447_for, 5¢-CCAGAAGGCGGCTCT GATTGCTGTGGGCTGAATTCACGC-3¢; and mutSox9447_rev, 5¢-GCGTGAATTCAGCCCACAGCAATCAGAG CCGCCTTCTGG-3¢ FEBS Journal 276 (2009) 2494–2504 ª 2009 The Authors Journal compilation...
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Tài liệu Báo cáo khoa học: Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc
... Metabolic role, purication, specicity, and kinetic mechanism J Biol Chem 266, 20658 20665 Parkin DW (1996) Purine-specic nucleoside N-ribohydrolase from Trypanosoma brucei brucei Purication, specicity, ... Ile Leu a Calculated using the program DSSP b Calculated according to Facchiano et al [48] c Calculated according to Facchiano et al [48] d Calculated using the program AVP sequence identity, ... from Crithidia fasciculata (CfIU-NH) [26] and Leishmania major (LmIU-NH) [9], the pyrimidine-specic NHs from E coli such as YeiK (Ec-YeiK) [29] and YbeK (Ec-YbeK) [30], and with the purine-specic...
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Tài liệu Báo cáo khoa học: Seed-based systematic discovery of specific transcription factor target genes pptx
... 5¢-TGGCTGGGGATTTCTCTGGG-3¢; OPTN forward, 5¢-ACCCGGGTCCCAGCCTCGAC-3¢; OPTN reverse, 5¢-GACAGCCAGCCGCTCCCTGC-3¢; SPI-B forward, 5¢-TCCAGCTCCTGTCCCATCTC-3¢; SPI-B reverse, 5¢-TGTCACATGGCAGGGATGGC-3¢; and CASP4 ... GTGAACCCCAGCCCTTCCTCGAT-3¢) and SPI-Brv (5¢-ACTGAAGCTTGGTGGTGCCGGGCGGGCTGT-3¢), and the SacI ⁄ HindIII tailed primers CASP4fw (5¢-ACT ACGCGTAGCAAAGAGTGCTGCCTCCTCCTTCCT-3¢) and CASP4rv (5¢-ACTCTCGAGTTCCCTGGTACAGAG ... (5¢-ACTGAGCTCCCGA CGACCCCAATTCAAATCG-3¢) and NFKBIArv (5¢-ACT GAAGCTTTGTGGGCTCTGCAGCGCCGC-3¢) The SPI-B and CASP constructs were generated using the MluI ⁄ XhoI tailed primers SPI-Bfw (5¢-ACTGAGCTC GTGAACCCCAGCCCTTCCTCGAT-3¢)...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... accessibility of the p53 CTDBS is critical for (sequence-nonspeci c) cisPtDNA recognition [34] On the other hand, sequencespeci c binding of p53 to the synthetic p53DBS PGM1 was inhibited in cisplatin-treated ... interaction that are mediated by the protein C- terminus, particularly the sequence-nonspeci c binding of p53 preferentially to cisPt-DNA [33,34] Another CTDBS-lacking tetrameric p53 construct, p53CT ... that speci cally recognize cisplatin-damaged DNA: a clue to anticancer activity of cisplatin FASEB J 12, 791–799 30 Kasparkova J & Brabec V (1995) Recognition of DNA interstrand cross-links of cis-diamminedichloroplatinum(Ii)...
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Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... detectable Proteins Liposomes ka (102 d1–7IC PE ⁄ PC PC PS ⁄ PC PtdIns ⁄ PC PG ⁄ PC PtdIns(3)P ⁄ PC PtdIns(3,4)P2 ⁄ PC PtdIns(3,4,5)P3 ⁄ PC PE ⁄ PC PC PS ⁄ PC PtdIns ⁄ PC PG ⁄ PC PtdIns(3)P ⁄ PC ... for IC and IC–CPY IC (A) or IC–CPY (B), the final concentration of which was lM, was added to PCbased liposomes (0.5 mgÆmL)1 of PE ⁄ PC, PC, PS ⁄ PC, and PtdIns ⁄ PC) in 20 mM Hepes (pH 7.2) containing ... cytoplasmic localization of IC in the logarithmic-phase cells Previous work on the inhibitory properties of IC in vitro, in which IC was shown to inactivate and interact with CPY under acidic...
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Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt
... 5¢-GGACTCAAGAGAGGAACCTG-3¢; F1b, 5¢-CT GCCTTGCTCCACACCTG-3¢; F 1c, 5¢-CTGCCGTCTCC GCCGCCACT-3¢; FU, 5¢-TCAAGCCCCGCTACATAG TT-3¢; and R3, 5¢-AGGAACCAAACACCAAGTGG-3¢ (Fig 2A) Luciferase promoter ... prostate cancer cells express only isoform 1, but not isoform [14], promoter is possibly inactivated in cancer cells There may be silencer sequence speci c for cancer cells at the promoter region Such ... 5¢-ATGGTGTCTGTGATGTTGCCG-3¢ and R2, 5¢-TTCTCACACTTCTGGTGCTC-3¢ DNA sequences of the PCR products were determined using an automatic DNA sequencer (DSQ-1000, Shimadzu, Kyoto, Japan) after cloning into...
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Tài liệu Báo cáo khoa học: How does hepatitis C virus enter cells? pptx
... in infectivity 80 9x increase in infectivity 0 4x increase in infectivity Fluorescence Fluorescence Fluorescence Fluorescence Fluorescence Fluorescence Fluorescence Fluorescence Fluorescence Fluorescence ... also facilitated binding of HCV clinical isolates to cells and their subsequent uptake into the endocytic compartment SR-BI-transfected CHO cells bound twice as many virions as parental CHO cells, ... DC-SIGN enhances infection of T -cells by capturing HIV on dendritic cells and transferring the virus to T -cells ASGPR is most commonly found on liver cells It facilitates the clearance of glycoproteins...
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Tài liệu Báo cáo khoa học: Parvalbumin deficiency in fast-twitch muscles leads to increased ‘slow-twitch type’ mitochondria, but does not affect the expression of fiber specific proteins doc
... AAG CTC ACT GG GTG AGG GAG ATG CTC AGT GTT GG AGTAACAATTTTCAGTGCTCCAAAC AATATACGGTTCATGGCAATACTGT CTACCCCGGCGCTGGAGTCAAC TCAGATGTTTTGCCACAAAGAGGTGCCTCCT CGA GCT TGC TTT ACA TCA GCC TGT GTC ATC ... GGT GAA GCC GGC TTC AAG GTT ACT TCG CGG TGG GGC ACC AGC TTG TAA TGG GGC GAA TCG TGG CAG TCA TCG ACC ACC ATG GGC TTT GGC GAC calculated from the gene bank databases For quantitative RT-PCR analyses, ... sarcoendoplasmic reticulum Ca2+-ATPase 2a Name Forward primer Reverse primer SERCA2a GAPDH CaN CaMKII COX I COX Vb F1-ATPase b GGC TCC ATC TGC TTG TCC ATG GAA GCG GTT ACT CCA GTA TTG GAG CTG AAC...
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Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
... CP-MAS 1 3C- NMR chemical shift values of ring and methyl carbons of native chitosan, LMWC and chito-oligomers Chemical shift values (p.p.m.) Sample CH3 C2 /C6 C3 /C5 C4 C1 -C O Chitosan LMWC (1 h) Chito-oligomers ... individual chains resulting from decrease in chain length, as evidenced by decreased molecular mass of LMWC The DA calculated using 13 C- NMR spectra was in accordance with the one calculated by IR-spectra ... detected using an RI detector Chito-oligomers and GlcNAc were used as standards Circular Dichroism (CD) CD spectra for native chitosan and LMWC (5 mgÆmL)1 in 0.1 M perchloric acid; path length, cm)...
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Tài liệu Báo cáo khoa học: Specific interaction between the classical swine fever virus NS5B protein and the viral genome pdf
... containing the Ó FEBS 2004 Interaction between CSFV NS5B and the genome (Eur J Biochem 271) 3893 A A 600 +RNA0 +RNA1 +RNA2 +RNA3 +RNA4 +RNA5 ACCTCGTATAC –RNA0 ACCTCGTATA –RNA1 ACCTC –RNA2 ACC ... ACCTC –RNA2 ACC –RNA3 AC –RNA4 –RNA5 B 600 CGGCCC CGGCC CGGC CGG C +RNA1 B +RNA2 +RNA3 +RNA4 +RNA5 – 1 C –RNA1 –RNA2 10 11 12 13 14 15 16 –RNA3 –RNA4 –RNA5 5 Fig RdRp assays containing various RNA ... respectively, the RNA templates containing the plus-strand 3¢ UTR with deletion of C , ÔCCÕ, ÔCCCÕ, ÔCCCGGÕ and the 21 nucleotide fragment at the 3¢ terminal –RNA1 to –RNA5 represent, respectively,...
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Tài liệu Báo cáo khoa học: A novel nuclear DNA helicase with high specific activity from Pisum sativum catalytically translocates in the 3¢fi5¢ direction docx
... inhibited by actinomycin C1 [22] Actinomycin C1 , a polypeptide containing the properties of an antibiotic, intercalates into dsDNA and thereby inhibits nucleic acid synthesis [23] Nogalamycin and daunorubicin ... inhibit cellular function Although many helicases have been characterized biochemically, it is often difficult to determine the in vivo role of a speci c helicase However, the biological roles ... DNA-interacting compounds on DNA unwinding activity of PDH120 The chemical structures of the compounds used have been described previously [16] Initially, each compound was used at a final concentration...
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