solid liquid interfaces studied by surface forces measurement
Effect of temperature and trace water on surface forces studied by liquid AFM
... a liquid close to a surface can be quite different from that in the bulk An interesting aspect of the solid- liquid interface is the induced molecular ordering in liquids between two solid surfaces ... Literature Survey 2.1 Solvation Forces Oscillatory forces at interfaces were first predicted in 1912 by W.B Hardy[40] His concept of the surface zone of a liquid, where the surface monomolecular layer ... solvation forces by Horn and Israelachvili [17] in 1980 Numerous experiments involving a variety of surfaces[1, 18-20], aqueous and non-aqueous liquid combinations[21-28] were conducted by Israelachvili...
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Báo cáo khoa học: Surface density of cellobiohydrolase on crystalline celluloses A critical parameter to evaluate enzymatic kinetics at a solid–liquid interface ppt
... process, the shape and surface area of the solid substrate should change with the reaction time By using surface density as a parameter, however, we can monitor the changes of surface area and compensate ... hydrolysis, presumably because of the differences of surface area and ⁄ or surface structure There are several techniques to estimate the surface area of solid cellulose from the amounts of FEBS Journal ... approach to evaluate cellulase kinetics on solid substrates by using the surface density of the enzyme (defined as the adsorbed amount of the enzyme divided by the maximum adsorption of the enzyme)...
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Báo cáo khoa học: Protein oligomerization induced by oleic acid at the solid–liquid interface – equine lysozyme cytotoxic complexes potx
... ELOA is shown by a solid line of the free dye in solution is shown by a dashed line (B) Interaction of ELOA with ThT The fluorescence spectrum of dye bound to ELOA is shown by a solid line and ... 1.8–8.9 lm ELOA it decreased by $ 20%, at a higher ELOA content of 12.4 lm it decreased by $ 40% Cell viability decreased by $ 70% upon the addition of 8.9 lm ELOA after h and by $ 80% after 24 h of ... a-lactalbumin (MAL), which indicates that the solid liquid interfaces of the chromatography column may mimic in vivo conditions Protein oligomerization induced by oleic acid Equine lysozyme within the...
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measurement of solid liquid interface temperature during pulsed excimer laser melting of polycrystalline silicon films
... thermal emission measured from the back side of the sample is examined by measuring the surface reflectance The surface reflectivity measurement also yields the melting duration due to a large reflectance ... measured by surface reflectance are in close agreement with those obtained from thermal emission measurement Considering that any possible thermal emission from the high-temperature solid silicon ... sample is also measured to verify that the surface temperature does not reach the boiling temperature ͑2628 K͒ In the case of surface evaporation, the solid liquid interface velocity increases only...
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Evaluation of the Characteristics of Microorganisms that Contribute to Denitrification in the Paddy Drainage Treatment Apparatus by Quinone Composition Measurement
... These values were calculated by the changes of nitrate-nitrogen concentration during the initial hour of the batch experiments, by the suspended charcoal chips volume, and by the quinone composition ... USA) Nitrate concentrations were analyzed by ion chromatography (DX-320, Dionex Corp., USA) Dissolved oxygen concentrations and pH levels were measured by the Galvanic DO sensor (DO-24, TOA DKK, ... denitrification rates (DNRs) by the microorganisms attaching charcoal chips were analyzed Charcoal chips were experimentally used in the water treatment apparatus for the paddy surface drainage Correlations...
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Tài liệu Báo cáo Y học: The solution structure and activation of visual arrestin studied by small-angle X-ray scattering pot
... 0.08 Data points obtained by dilution of a 140-lM stock solution are indicated by circles, while those obtained by further concentration of the stock solution are indicated by squares All of the ... scattering at S values greater than 0.2 nm)1 could be produced by the presence of the phosphopeptide on the surface of arrestin and/or by changes in ÔlocalÕ arrestin structure These results are consistent ... predicted scattering (solid curve) of the ÔAAÕ dimer formed by interaction between the C-terminal domains of arrestin monomers (B) Structure (ribbon diagram) and predicted scattering (solid curve) of...
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Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx
... corroborated by the fact that mutations increasing thermostability while maintaining low-temperature activity are extremely rare [25] In fact, this rigidication has been revealed by HD exchange ... structure was recently obtained [28] Also, RdDg is highly stabilized by diglycerol phosphate (DGP), a solute accumulated by the hyperthermophilic archaeon Archaeoglobus fulgidus [7,29] Addition ... principal axis for use in the model-free analysis The parameters, selected by extensive Monte-Carlo simulations as described by Mandel et al [31], are summarized in Table After model selection, both...
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Báo cáo khoa học: High-pressure effects on horse heart metmyoglobin studied by small-angle neutron scattering pdf
... scattering data the actual protein contrast given by Eqn has been replaced by the quantity ! NA K0 Pị ẳ bp q0 b Pịvp 0:1ị 4ị Mp where q0b P ị is given by Eqn Using this expression and the virial expansion ... present SANS measurements at the lowest pressure is not due to the ellipsoid shape of Mb [37,42] but to the presence of electric charges on the protein surface and possibly, to a high surface hydration ... experiments to ensure that the protein was almost fully liganded with N3 [36] This was checked by absorbance measurements in the visible region The p2H of the solution was measured after ligandation...
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Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx
... stability were also studied Replacing C67 with serine (designated WT* in Table 1) reduced the catalytic efciency (kcat Km) by 45% and increased temperature stability (Tm) of the enzyme by C Replacing ... of the catalytic alkoxide ion by pointing the nitroxide toward the nearby enzyme active site The mobility of C67R1 puts this residue inside the well-dened helix surface mobility region of the ... these interactions originate from, either by mutation of the possibly interacting residues or by using double site-directed spin-labeling for distance measurements between the insert region and...
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Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Blue-light active flavoproteins studied by electron paramagnetic resonance pptx
... triplet states studied by time-resolved EPR spectroscopy J Photochem Photobiol C Photochem Rev 4, 109–124 63 Jordan SP & Jorns MS (1988) Evidence for a singlet intermediate in catalysis by Escherichia ... triplet states or radical pairs generated during (photo-)chemical reactions can be favorably studied by measuring the EPR signal intensity as a function of time at a fixed value of the external ... time resolution can be increased by one order of magnitude, which makes the method well suited to the study of transient free radicals on a microsecond time scale By removing the magnetic-field modulation...
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Growth of amorphous silicon nanowires via a solid–liquid–solid mechanism
... eutectic liquid alloy droplets Because of the relatively high solubility of Si in Si Ni eutectic alloy, more Si atoms will diffuse through the solid Žsubstrate liquid interface into the liquid ... through the substrate liquid ŽSL interface into the liquid droplets, and growth of SiNWs through the liquid wire ŽLS interface; Žd final state of SiNW growth The smooth surface of the original ... liquid interface into the liquid phase Ži.e., the Ni Si droplets A second liquid solid Žnanowire interface will form when the liquid phase becomes supersaturated due to thermal or compositional fluctuations,...
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Controlled growth of oriented amorphous silicon nanowires via a solid–liquid–solid (SLS) mechanism
... using Si substrate as Si source via a solid liquid solid mechanism by heating They have uniform length and diameter The growth is explained by solid liquid solid model This synthesis method of ... eutectic liquid alloy droplets Because of the relatively high solubility of Si in Si2 Ni eutectic alloy, more Si atoms will di use through the solid (the substrate) liquid interface into the liquid- phase ... compositional uctuations, resulting in the growth of SiNWs Because this growth process involves solid liquid solid phases, it is named as a SLS growth, which is in fact an analogy of the VLS mechanism...
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Báo cáo khoa học: Netropsin interactions in the minor groove of d(GGCCAATTGG) studied by a combination of resolution enhancement and ab initio calculations pot
... the minor groove is the widening of this groove, which is confirmed by the reported structure The minor groove width is defined by the shortest H4¢-H5¢ distances between the two opposite DNA strands ... properly account for the dispersion attraction [28,29,44,45] The contact area of the NAE has been studied by evaluating the effect of adenine A25, thymine T8, guanine G9 and one water molecule, W57, ... was supported by the European Community Research Infrastructure Action under the FP6 ‘Structuring the European Research Area Programme’ contract number RII3 ⁄ CT ⁄ 2004 ⁄ 5060008 and by the Fund...
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Báo cáo khoa học: Catalytic transformations of supercoiled DNA as studied by flow linear dichroism technique doc
... topoisomerization studied by FLD a local alteration of DNA geometry, seems reasonable for the description of the substrate properties of scDNA caused by supercoiling which might be recognized by topoisomerases ... capture of a DNA double helix by an ATP-dependent protein clamp: a key step in DNA transport by type II DNA topoisomerases Cell 71, 833–840 DNA topoisomerization studied by FLD 41 Morris SK & Lindsley ... demonstrated by other plasmids under study, including pTM and FEBS Journal 272 (2005) 6336–6343 ª 2005 The Authors Journal compilation ª 2005 FEBS 6337 DNA topoisomerization studied by FLD A Gabibov...
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Báo cáo khoa học: Structural function of C-terminal amidation of endomorphin Conformational comparison ofl-selective endomorphin-2 with its C-terminal free acid, studied by 1 H-NMR spectroscopy, molecular calculation, and X-ray crystallography pot
... amidation Solution conformation by NMR spectroscopy and simulated annealing calculation The conformational features of EM2 and EM2OH, obtained by the present NMR measurements and molecular modeling ... [Chx2]EM2 (black) The backbone chains are represented by thick bonds, and the intramolecular hydrogen bonds are shown by dotted lines stabilized by (Tyr)C ¼ O HN(C-terminal amide) as the bioactive ... biological function of naturally occurring opioid peptides could be explained by the ‘message-address concept’ proposed by Schwyzer [10] According to this concept, EM2 could be divided into a message...
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solid-liquid filtration and separation technology
... is achieved by means of a mass balance on the solid and liquid entering the filter system, Mass of solids in the filter cake: Mass of liquid in the filter cake: Mass fraction of solids in the ... industry 8 I Solid Liquid Sepurution Technology Here, gravity sedimentation, involving a difference m density between solids and liquids, is used to produce an essentially clear overflow of liquid ... s-' m s-l m s? m Greek Symbols Y P P Ps Surface tension Liquid viscosity Fhid density Solid density A g l r velocity nua Nm Pa s kg m-3 kg m-' S ' Solid- LiquidFiItration and Separa tion TechnoIogy...
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Báo cáo sinh học: " Rapid label-free identification of mixed bacterial infections by surface plasmon resonance" pdf
... completed, the chip surface (precoated with probes) was regenerated by washing with 100 μL of 0.01% SDS and 100 μL of mM HCl at a speed of 50 μL/min To equilibrate the chip surface, system buffer ... interface between the chip surface and an ambient medium Hybridization between a probe immobilized on the chip surface and its target may cause the conformational changes in the surface of the gold ... Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance Nat Biotechnol 2001, 19:51-55 Chu LQ, Forch R, Knoll W: Surface- plasmon-enhanced fluorescence spectroscopy...
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báo cáo hóa học:" Rapid label-free identification of mixed bacterial infections by surface plasmon resonance" pptx
... completed, the chip surface (precoated with probes) was regenerated by washing with 100 μL of 0.01% SDS and 100 μL of mM HCl at a speed of 50 μL/min To equilibrate the chip surface, system buffer ... interface between the chip surface and an ambient medium Hybridization between a probe immobilized on the chip surface and its target may cause the conformational changes in the surface of the gold ... Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance Nat Biotechnol 2001, 19:51-55 Chu LQ, Forch R, Knoll W: Surface- plasmon-enhanced fluorescence spectroscopy...
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Báo cáo hóa học: " Enhanced photo-sensitivity through an increased light-trapping on Si by surface nano-structuring using MWCNT etch mask" pptx
... through an increased light-trapping on Si, which is achieved by increasing surface roughness and suppressing reflection losses through a surface nano-texturing using inductively coupled plasma (ICP) ... letters in (a-d) 2D and 3D AFM images are (x) × (y) μm2 that the surface nano-structuring by ICP etching using MWCNT etch mask provides surface patterns that are sensitive to photons of shorter wavelengths ... wavelength, the reflectance is expected to be less affected by the surface roughness as the wavelength becomes comparable with the surface features Overall, the sample D showed the highest RMSR...
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Báo cáo hóa học: " Layer-dependent nanoscale electrical properties of graphene studied by conductive scanning probe microscopy" docx
... topography and the phase shift that is directly linked to the electrical force gradient by using a two-pass method By SCM, the capacitance variation ΔC between the tip and the underlying semiconductor ... both images As the ac voltage variation (ΔV) is kept constant in all measurements, the capacitance variation (ΔC) obtained by multiplying dC/dV amplitude with ΔV was adopted afterwards instead ... is dependent on n, as shown in Table On the other hand, it was reported by Yu et al that the work function could be tuned by the gate voltage, where they found that SLG showed larger work function...
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