role of glial cells genetic basis and relevance of brain imaging for diagnosis
Báo cáo khóa học: Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 ppt
... and 125 ng for fhx/P25 from cocoons of C108 and the transgenic line L6 · 7, or lg for H-chain, 10 lg for L-chain and 100 lg for fhx/P25 from naked-pupa cocoons of Nd-sD and cocoons of the transgenic ... weight of cocoons produced (n ¼ 50 each) was 110 mg for L6 · and 20.1 mg for L7-4 as compared with 187 mg for C108 and 17.7 mg (consisting mostly of sericin) for Nd-sD Proteins from cocoon shells of ... significance of the assembly of the elementary unit of fibroin in ER It was demonstrated in this study that the elementary unit of fibroin, having the same molar ratio of H- and L-chains, and fhx/P25 as of...
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The Role of Consulting Psychiatrists for Obstetric and Gynecologic Inpatients pptx
... consultations per year, all of which are discussed and reviewed in a weekly consultation psychiatric service conference led by a professor and a senior attending psychiatrist The diagnosis and treatment recommendations ... reevaluated and confirmed in this meeting 58 consultation records was performed, and the data collected included baseline data (age and marital status), physical diagnosis, psychiatric diagnosis, ... baseline data, physical diagnosis, psychiatric diagnosis, reasons for referral, and intervention recommended were analyzed using the Statistical Package for Social Science (SPSS) for Windows, Version...
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Báo cáo y học: "Clinical review: The role of biomarkers in the diagnosis and management of communityacquired pneumonia" potx
... proposed for the diagnosis and management of CAP in the future Early microbial diagnosis and susceptibility testing Molecular biomarkers for rapid microbial diagnosis using real-time PCR and similar ... score, and in 19% of patients was evident before diagnosis could be made on the basis of the scoring system [61] Patients with severe CAP show abnormalities of coagulation [67], and an absence of ... permits early diagnosis and appropriate therapy, and use of the procedure will probably become the standard of care in the next few years Indicators of prognosis CAP is the leading cause of death...
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The role of precautionary labelling for food allergens and the care of children with food allergies
... foods are often used by parents of young children because of their accessibility and ease -of- use Use of processed foods is more complicated for parents of children with food allergy because of concerns ... task at hand and have provided me with educational and emotional support I will always be grateful for the time that I have spent here with you and for the fine example of the pursuit of excellence ... the PhD thesis entitled ‘’The Role of Precautionary Labelling for Food Allergens and the Care of Children with Food Allergies” submitted for the degree of Doctor of Philosophy is no more than...
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Tài liệu Báo cáo Y học: Role of sulfoquinovosyl diacylglycerol for the maintenance of photosystem II in Chlamydomonas reinhardtii ppt
... were dissolved in chloroform/methanol (2 : 1, v/v) and stored at )20 °C Supplementation of algal cells and thylakoid membranes with SQDG SQDG for supplementation to hf-2 cells and their thylakoid ... ethanol was used and carefully diluted with : 100 (v/v) of the reaction mixture For the preparation of thylakoid membranes for PSII activity, mL of cell suspension was sonicated for s in 25 mM ... concentrations (0–100 lM) of SQDG were added to the cells on agar plates containing 135 nM diuron For lipid analysis of the cells cultured in the presence of SQDG, the cells were washed three times...
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Báo cáo khoa học: Role of conformational flexibility for enzymatic activity in NADH oxidase from Thermus thermophilus pptx
... Tryptophan residues in the monomeric form of NADH oxidase are spatially separated from the location of the avin cofactor The distance between N1 of the avin cofactor and Ne1 of tryptophans 47, 52, ... activity and the conformation of the active site of NADH oxidase are very sensitive to both the ionic strength and the type of anions (G Zoldak, M Sprinzl and E Sedlak, unpublished results) Therefore, ... water molecule forms hydrogen bonds to the backbone nitrogen of Trp47 and to O2 of the ribityl chain and is located in the middle of the avin system with distances of 3.3 A to N10 and 3.7 A to...
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Constructive role of internal noise for the detection of weak signal in cell system
... ability of the system in detection of the weak signal, especially when the frequency of the signal is around that of the intrinsic oscillation of the system And, because the frequency of the intrinsic ... μm3 for a wide range of signal amplitudes and control parameters, which is of the same order of real living cells in vivo Since the internal noise in living cell systems cannot be ignored and ... stochastic spike trains, and it could be of biological significance because it is related to the time precision of information processing A larger value of CM means more closeness of the spike train...
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Multifunctional nanoparticles of biodegradable polymers for diagnosis and treatment of cancer
... NANOPARTICLES OF BIODEGRADABLE POLYMERS FOR DIAGNOSIS AND TREATMENT OF CANCER PAN JIE (M ENG., TIANJIN UNIVERSITY) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ... biocompatibility and stability of QDs, prolong circulation time of QDs, and improve the selective uptake and imaging specificity, sensitivity of cancer cells with minimal XI cytotoxicity to normal cells ... nanoparticles of biodegradable polymers for QDs formulation for targeted and sustained imaging In order to reduce the cytotoxicity of QDs, two methods were used One method is by surface modification and...
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ON THE ROLE OF VOCAL EMOTIONS FOR VERBAL MEMORY
... means and standard deviations of mean dprime scores valence ratings for words spoken with the neutral and sad prosody (Experiment 1) .22 Table Table illustrating means and standard deviations of ... Finally, support for the preferential processing of emotional relative to neutral prosody comes from neuroimaging studies For example, a study by Grandjean and colleagues (2005) examined brain responses ... data was processed off-line To remove slow drifts and high frequency noise, a digital band pass filter was applied with a high frequency cutoff of 0.8 and a low pass frequency cutoff at 40 Hz QRS...
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Báo cáo y học: "Provider-initiated HIV testing in rural Haiti: low rate of missed opportunities for diagnosis of HIV in a primary care clinic" ppsx
... number of encounters at the clinic prior to HIV diagnosis, number of days between first visit and diagnosis, and CD4 cell count at time of diagnosis Any visit to the clinic before the diagnosis of ... was performed using SAS 9.1™software We used the Wilcoxon Rank Sum Test to assess differences in CD4 cell count at the time of diagnosis between those with and without a delay in diagnosis, and ... approximately 23% of the population between the ages of 15 and 49 years The Partners In Health/Ministry of Health clinic is the only formal healthcare center in the area and at the time of the study...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... understanding of the molecular mechanisms of ethanol at low concentration in HepG2 cells, and a novel approach for the identification of potential targets in the diagnosis and treatment of human ... expression profile of mM ethanol concentration treated HepG2 cells (group 1) and control cells (HepG2 cells without treatment; group 2) exposed for a h period Data are presented as a median of the ... potential targets in the diagnosis and treatment of human hepatocellular carcinoma Materials and methods Cell culture and reagents Based on the reported alteration of gene expression of primary human...
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Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx
... SH-SY5Y cells and similarly by about tenfold in SK-N-SH and cells Further deletion to +42 increased transcription in both cells lines: about fivefold in SH-SY5Y cells and twofold in SK-N-SH cells ... with the PS1 promoter and 1.5 mL of 12.5% (v/v) glycerol was added for 90 s on SK-N-SH cells and 60 s on SH-SY5Y cells Glycerol was Total RNA was prepared from SK-N-SH cells and removed after dilution ... elements play a major role in determining both the level of expression and the location of the start of transcription of PS1 In the Ets family of transcription Ó FEBS 2004 Regulation of the presenilin...
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Báo cáo hóa học: " Role of protease-activated receptor-2 on cell death and DNA fragmentation in Helicobacter pylori-infected gastric epithelial cells" ppt
... epithelial cells AGS cells were treated with SBTI and cultured in the absence or the presence of H pylori at a bacterium /cells ratio of 300:1 for 30 minutes The levels of phospho-specific and total forms ... and total forms of MAPK (p38, ERK1/2, JNK1/2) As shown figure 3, levels of phospho-specific forms of p38, ERK1/2, and JNK1/2 increased by H pylori in AGS cells H pylori did not affect total forms ... AGS cells AGS cells were transfected with PAR-2 AS ODN or S ODN for 24 hours and cultured in the absence or the presence of H pylori at a bacterium/ cells ratio of 300:1 for hours The levels of...
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báo cáo hóa học: " A role for DNA-dependent activator of interferon regulatory factor in the recognition of herpes simplex virus type 1 by glial cells" pot
... significant role in the inflammatory responses of resident CNS cells to HSV-1 challenge Materials and methods Isolation of primary murine microglia and astrocytes Murine neonatal brain microglia and ... prepared and analyzed for the presence of DAI, STING, RIP3, and viral gG1 by immunoblot analysis In vitro stimulation of microglia and astrocytes with the DAI ligand, B-DNA Poly(dA:dT) double-stranded ... supernatants and whole cell protein isolates were collected for analysis For comparison purposes, cells were exposed to transfection reagent alone or HSV-1 (MOI of and 10) for 24 hours Quantification of...
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Báo cáo khoa học: "The role of Bcl-xL and nuclear factor-kB in the effect of taxol on the viability of dendritic cells" ppsx
... density of each band was divided by that of the β-actin band, and the ratio at h was set at 100% (B) Fig NF-κB involvement in the taxol-induced effects on dendritic cells (DCs) The mobilization of ... KRF-2004-202-E00184) and performed under the program of the Basic Atomic Energy Research Institute (BAERI), which is a part of the Nuclear R&D Programs funded by the Ministry of Science & Technology (MOST) of ... Steinman RM Dendritic cells and the control of immunity Nature 1998, 392, 245-252 Blanco P, Palucka AK, Pascual V, Banchereau J Dendritic cells and cytokines in human inflammatory and autoimmune diseases...
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Báo cáo khoa học: " Role of protease inhibitors and acylation stimulating protein in the adipogenesis in 3T3-L1 cells" potx
... 37 C for h After o heating at 94 C for min, PCR amplification was performed with 2.5 units Taq polymerase (Perkin-Elmer, USA), mM MgCl2 and 50 pmol of forward and reverse primers specific for ... 72 C for and reverse transcribed using 100 units of Moloney murine leukemia virus reverse transcriptase (Gibco, USA), 50 pmol of poly (dT) primer and 20 nmol of o dNTPs in a total volume of 10 ... effect of PI on lipids accumulation in adipocytes, PI was added from day to day in doses of ×300, ×200, ×150 and ×100 To test the effect of PI and ASP, on day of differentiation, the cells were...
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Báo cáo y học: "Synergistic role of c-Myc and ERK1/2 in the mitogenic response to TGF-1 in cultured rat nucleus pulposus cells" ppsx
... for p21, 27 cycles for p27, 30 cycles for c-myc and 26 cycles for -actin Each cycle included denaturation at 95°C for 15 s, followed by annealing and extension at 61°C for A total of 10 L of ... uptake With regard to intervertebral disc cells, the enhancement of colony formation of human annulus fibrosus cells and increase in density of nucleus pulposus cells in threedimensional scaffold cultures ... nucleus pulposus cells and articular chondrocytes Further analyses of nucleus pulposus cells indicated that levels of p21 mRNA decreased with TGF1 treatment and that levels of c-Myc mRNA were...
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Báo cáo y học: "The role of regulatory T cells in antigen-induced arthritis: aggravation of arthritis after depletion and amelioration after transfer of CD4+CD25+ T cells" doc
... cells had higher levels of IgGs (Fig 6d) R296 Figure Modulation of antigen-induced arthritis (AIA) by transfer of regulatory T cells (Treg cells) cells (Treg cells) Amelioration of clinical and ... properties of CD4+CD25+ Treg cells Because the mechanism of suppression of Treg cells in vitro is cell contact dependent, localization of cells might be important for their regulatory activity Therefore, ... Migration behaviour of regulatory T cells (Treg cells) (a) CD4+CD25+ cells) and CD4+CD25- cells were purified by fluorescence-activated cell sorting (FACS) and labelled with 111In Cells (1 × 106)...
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Báo cáo y học: "Role of regulatory T cells in experimental arthritis and implications for clinical use" potx
... after withdrawal of anti-TNF-α [19] and thus, despite the dampening of joint inflammation and the reinstatement of fully functional CD4+CD25+ Treg, RA is still not cured These strands of evidence ... generated and expanded in vitro, might tip the balance and allow a true curative action in animal models of T1D [12], and much work is now directed to this area [20] Two recent studies in T1D and experimental ... highlighted the potential of this option by genetic manipulation of T cells [21,22] However, this might not be an easy road to follow in humans because not all autoantigen-specific T cells seem to be...
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