... investigated. Two point-mutated proteins, with a singlebase substitution of alanine for tryptophan (W14 0A) and alanine for lysine (K13 3A) , and two truncatedfragment proteins, with fragments ... that tryptophan at position140 plays an important rolein maintaining protein ter-tiary integrity. Figure 4A shows how tryptophan (loop1) may interact with loop 2 and loop 3. These inter-actions ... that contains onedomain and no disulfide bonds. Its stability in the native state may bemaintained mainly by key amino acids. In this study, two point-mutatedproteins each with a single base substitution...
... and isola-ted TAM1 and TAM2 cells, in either of which TAM67was detected (Fig. 6A) . Because TAM67 lacks thetransactivation domain of c-Jun (amino acids 1–122),but retains the DNA binding and ... expression of c-Fos and c-Jun, via the MAPkinase signaling pathway.TAM67 retains the DNA binding and leucine-zipperregion of c-Jun, but it lacks the transactivation domain of c-Jun (amino acids 1–122). ... ADR-induced apop-tosis, suggesting that EGF transmitted the antiapoptotic signal in such a way that it activated AP1 via a MAP kinase signaling pathway. TMK-1cells stably transfected with TAM67,...
... in cold adaptation of an alkaline phosphataseKonstantinos Mavromatis1,*, Iason Tsigos2,*, Maria Tzanodaskalaki2, Michael Kokkinidis1,3and Vassilis Bouriotis1,21Department of Biology, ... group of Ala261 side-chain could produce stericclashes with the aromatic ring of Tyr269, and theseunfavorable interactions could lead to a decrease of localflexibility and an increased E a value.The ... mutant alkaline phosphatases. (A) Temperature dependence of kcat of TAB5 (d), mutants G26 1A (r), G26 1A/ Y26 9A (j)andE.coli (·) alkaline phosphatases attemperature range 5–25 °C. kcatvalues...
... lg of DNA per 100 mm dish and2 lg of DNA per 30 mm dish. The ratio of DNA codingdonor to DNA coding acceptor was 1 : 1 or 1 : 2.Membrane preparation and radioligandbinding assayFor binding ... thearginine-rich epitope (217RRRRKR222) of the third intra-cellular loop were exchanged (D2R1: 217AARRKR222,D2R2: 217AAAAKR222, D2R3: 217AAAAAA222), as wellas one variant of the dopamine ... density of the D1MUT (404AA405) wasTable 1. Binding parameters for the dopamine receptors. For dopa-mine D2receptor binding, the statistical significance was evaluatedusing a one-way ANOVA,...
... guxx@nidcd.nih.govPresent addresses*School of Veterinary Medicine,Yangzhou University, Yangzhou,Jiangsu, China†Defence Research and DevelopmentCanada-Suffield, Medicine Hat, Alberta,CanadaDatabaseThe nucleotide ... wereobtained via 3070xl DNA analyzer (Applied Biosystems,Foster City, CA) and analyzed with dnastar software(DNASTAR Inc., Madison, WI).Transposon mutagenesis and identification of lgt3 gene In ... Hansen for providing strain O35E,Wenzhou Hong for assisting in the animal challenge,and Robert Morell and Yandan Yang for helping in DNA sequencing. This research was supported by theIntramural...
... of A DNA binding domain B Ligand binding domain Fig. 1. Sequence alignment. (A) Alignment of DNA binding domain (C domain) and itsC-terminal extension. (B) Alignment of ligandbinding domain ... gene assay inin vivo results.Experimental proceduresParasitesThe NMRI strain of S. mansoni was maintained in snails(Biomphalaria glabrata) and Syrian golden hamsters(Mesocricetus auratus ... Yeast one and two-hybrid assays. (A) Yeast one hybridassay showing that SmNR1 contains an autonomous transactivationfunction inA ⁄ B domain. Individual AH109 yeast colonies obtainedfrom an...
... site and plays a critical rolein substrate binding [41]. Substrate or sub-strate analogues may anchor this loop upon binding,making a substantial conformational change comparedto the apo structure.Table ... B, Sawka-Dobrowolska W, GancarzR, Kucharska-Zon˜M, Latajka R, Amrhein N, MiziakP & Szczepanik W (2004) Experimental and ab initiocalculated structures of 2-aminoindane-2-phosphonicacid, ... Molecularphenotyping of the PAL1 and PAL2 mutants of Arabid-opsis thaliana reveals far-reaching consequences onphenylpropanoid, amino acid, and carbohydrate meta-bolism. Plant Cell 16, 2749–2771.Tyr-loop in...
... presence ofan additional phosphate group (1032), and additional PEtn moiety (1075), an additional phosphate group and an additional PEtn moiety (1155) and one additional phosphate group andtwo additional ... 227–244.24. Aspinall, G.O., Monteiro, M .A. & Pang, H. (1995) Lipo-oligo-saccharide of Campylobacter lari type strain ATCC 35221.Structure of the liberated oligosaccharide and an associatedextracellular ... The remaining resolved residues in the a- anomeric region at 5.07 and 5.39 p.p.m. and a minorsignal at 5.50 p.p.m. were determined to be gluco-pyranose amino sugars, from the appearance of theirspin...
... GGAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC3+wtGGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC3+YIRNGGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG3– GGAATTCTGCAGTTAGCATTTgagCTCtccCTTGCACAAgAAGTCCGGÓ FEBS 2003 A novel ... GGGCAAgcttgCTaTTcCCTCCTACTCCTcTTACGGATGCTACTGCGGCtgGGGGGG 2a+ GACTGCAaCCCCAAAtCGGACAG2b+ CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTGaAAAAGGC2 GGAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC3+wtGGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC3+YIRNGGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG3– ... study, interaction of group IIAsPLA2toxins with CaM was evaluated using a set of AtxAmutants, including this quadruple (KKML) mutant, as wellas recombinant DPLA2and its quadruple reciprocal(YIRN)...
... (GAGCTGAATTCTTTGGTCA) containing an EcoRI site (in italics), and dch-II (ATGTAAGATCTGAACTGGACTTCG) containing a BglII site (in italics),cloned into pT7Blue, excised with EcoRI and BglII, andthen ... primer, GGATCCGGACCAGTTGGTGATTTT, and antisense primer, GGATCCTTAGAAAAACTCATCGAGC (BamHI recognition are shown in italics). The amplified KmRwas excised with BamHI andthen inserted into the ... PCR using pADH10 andthe following two oligonucleotides as a template and asprimers, respectively. The sense primer (CAGGAGAATTCAAAATGGG) contained an EcoRI recognition site (in italics), and...